Nuclear Ribosomal DNA ITS-2 Sequence Characterization of Fasciola hepatica and Galba truncatula
Background: Human fascioliasis is an important health problem in the province of Gilan, at the Caspian Sea, Iran. There is the overlapping of both fasciolid species, Fasciola hepatica and F. gigantica. Recent studies on both domestic animal and lymnaeid infection furnished evidence suggesting that...
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| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2007-12-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/2089 |
| _version_ | 1818904824513560576 |
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| author | K Ashrafi J Massoud K Holakouie Naieni MA Jo-Afshani M Mahmoodi N Ebadati SM Rezvani P Artigas MD Bargues S Mas-Coma |
| author_facet | K Ashrafi J Massoud K Holakouie Naieni MA Jo-Afshani M Mahmoodi N Ebadati SM Rezvani P Artigas MD Bargues S Mas-Coma |
| author_sort | K Ashrafi |
| collection | DOAJ |
| description | Background: Human fascioliasis is an important health problem in the province of Gilan, at the Caspian Sea, Iran. There is the overlapping of both fasciolid species, Fasciola hepatica and F. gigantica. Recent studies on both domestic animal and lymnaeid infection furnished evidence suggesting that F. gigantica and Radix gedrosiana may be the main fasciolid and lymnaeid involved in the disease in that province, controversy still being there concerning the presence and importance of F. hepatica and other lymnaeid species. The present paper includes the results of studies on Galba truncatula and the first finding of natural infection by F. hepatica in Gilan proved by molecular studies.
Methods: Snail collections were carried out in summer, when their populations present the highest densities. Surveys on lymnaeids furnished the finding of a lymnaeid snail infected by trematode rediae and cercariae in the mountains of Talesh, in the Asalem district, western Gilan.
Results: Nuclear ribosomal DNA ITS-2 sequences proved that they were F. hepatica and G. truncatula. The liver fluke ITS-2 sequence was identical to that of F. hepatica from Spain and the Northern Bolivian Altiplano and that of G. truncatula to the haplotype H-2 known in Portugal, Spain, France and The Netherlands.
Conclusion: This genetic characterization suggests that both may be also involved in human fascioliasis infection in Gilan. |
| first_indexed | 2024-12-19T21:13:35Z |
| format | Article |
| id | doaj.art-5d70d8061300491a94676d86518c9bc5 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-19T21:13:35Z |
| publishDate | 2007-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-5d70d8061300491a94676d86518c9bc52022-12-21T20:05:26ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932007-12-01364Nuclear Ribosomal DNA ITS-2 Sequence Characterization of Fasciola hepatica and Galba truncatula K Ashrafi0 J Massoud1 K Holakouie Naieni2 MA Jo-Afshani3 M Mahmoodi4 N Ebadati5 SM Rezvani6 P Artigas7 MD Bargues8 S Mas-Coma9 Background: Human fascioliasis is an important health problem in the province of Gilan, at the Caspian Sea, Iran. There is the overlapping of both fasciolid species, Fasciola hepatica and F. gigantica. Recent studies on both domestic animal and lymnaeid infection furnished evidence suggesting that F. gigantica and Radix gedrosiana may be the main fasciolid and lymnaeid involved in the disease in that province, controversy still being there concerning the presence and importance of F. hepatica and other lymnaeid species. The present paper includes the results of studies on Galba truncatula and the first finding of natural infection by F. hepatica in Gilan proved by molecular studies. Methods: Snail collections were carried out in summer, when their populations present the highest densities. Surveys on lymnaeids furnished the finding of a lymnaeid snail infected by trematode rediae and cercariae in the mountains of Talesh, in the Asalem district, western Gilan. Results: Nuclear ribosomal DNA ITS-2 sequences proved that they were F. hepatica and G. truncatula. The liver fluke ITS-2 sequence was identical to that of F. hepatica from Spain and the Northern Bolivian Altiplano and that of G. truncatula to the haplotype H-2 known in Portugal, Spain, France and The Netherlands. Conclusion: This genetic characterization suggests that both may be also involved in human fascioliasis infection in Gilan.https://ijph.tums.ac.ir/index.php/ijph/article/view/2089IinfectionrDNA ITS-2 sequences |
| spellingShingle | K Ashrafi J Massoud K Holakouie Naieni MA Jo-Afshani M Mahmoodi N Ebadati SM Rezvani P Artigas MD Bargues S Mas-Coma Nuclear Ribosomal DNA ITS-2 Sequence Characterization of Fasciola hepatica and Galba truncatula Iranian Journal of Public Health Iinfection rDNA ITS-2 sequences |
| title | Nuclear Ribosomal DNA ITS-2 Sequence Characterization of Fasciola hepatica and Galba truncatula |
| title_full | Nuclear Ribosomal DNA ITS-2 Sequence Characterization of Fasciola hepatica and Galba truncatula |
| title_fullStr | Nuclear Ribosomal DNA ITS-2 Sequence Characterization of Fasciola hepatica and Galba truncatula |
| title_full_unstemmed | Nuclear Ribosomal DNA ITS-2 Sequence Characterization of Fasciola hepatica and Galba truncatula |
| title_short | Nuclear Ribosomal DNA ITS-2 Sequence Characterization of Fasciola hepatica and Galba truncatula |
| title_sort | nuclear ribosomal dna its 2 sequence characterization of fasciola hepatica and galba truncatula |
| topic | Iinfection rDNA ITS-2 sequences |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/2089 |
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