Biomolecular Screening of <i>Pimpinella anisum</i> L. for Antioxidant and Anticholinesterase Activity in Mice Brain
Hundreds of the plants have been explored and evaluated for antioxidant and anti-amnesic activities, so far. This study was designed to report the biomolecules of <i>Pimpinella anisum</i> L. for the said activities. The aqueous extract of dried <i>P. anisum</i> seeds was frac...
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/5/2217 |
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| author | Aamir Mushtaq Fatima Habib Rosana Manea Rukhsana Anwar Umar Farooq Gohar Muhammad Zia-Ul-Haq Mobasher Ahmad Claudia Mihaela Gavris Liana Chicea |
| author_facet | Aamir Mushtaq Fatima Habib Rosana Manea Rukhsana Anwar Umar Farooq Gohar Muhammad Zia-Ul-Haq Mobasher Ahmad Claudia Mihaela Gavris Liana Chicea |
| author_sort | Aamir Mushtaq |
| collection | DOAJ |
| description | Hundreds of the plants have been explored and evaluated for antioxidant and anti-amnesic activities, so far. This study was designed to report the biomolecules of <i>Pimpinella anisum</i> L. for the said activities. The aqueous extract of dried <i>P. anisum</i> seeds was fractionated via column chromatography and the fractions so obtained were assessed for the inhibition of acetylcholinesterase (AChE) via in vitro analysis. The fraction which best inhibited AChE was so named as the <i>P. anisum</i> active fraction (P.aAF). The P.aAF was then chemically analyzed via GCMS, which indicated that oxadiazole compounds were present in it. The P.aAF was then administered to albino mice to conduct the in vivo (behavioral and biochemical) studies. The results of the behavioral studies indicated the significant (<i>p</i> < 0.001) increase in inflexion ratio, by the number of hole-pokings through holes and time spent in a dark area by P.aAF treated mice. Biochemical studies demonstrated that the oxadiazole present in P.aAF on one hand presented a noteworthy reduction in MDA and the AChE level and on the other hand promoted the levels of CAT, SOD and GSH in mice brain. The LD<sub>50</sub> for P.aAF was calculated as 95 mg/Kg/p.o. The findings thus supported that the antioxidant and anticholinesterase activities of <i>P. anisum</i> are due to its oxadiazole compounds. |
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| last_indexed | 2024-03-11T07:16:31Z |
| publishDate | 2023-02-01 |
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| series | Molecules |
| spelling | doaj.art-5d74e440ea1642c3b57509e01ac4d7c92023-11-17T08:13:36ZengMDPI AGMolecules1420-30492023-02-01285221710.3390/molecules28052217Biomolecular Screening of <i>Pimpinella anisum</i> L. for Antioxidant and Anticholinesterase Activity in Mice BrainAamir Mushtaq0Fatima Habib1Rosana Manea2Rukhsana Anwar3Umar Farooq Gohar4Muhammad Zia-Ul-Haq5Mobasher Ahmad6Claudia Mihaela Gavris7Liana Chicea8Department of Pharmaceutical Sciences, Government College University, Lahore 54000, PakistanGulab Devi Institute of Pharmacy, Gulab Devi Educational Complex, Lahore 54000, PakistanFaculty of Medicine, Transilvania University of Brasov, 500036 Brasov, RomaniaDepartment of Pharmacology, Punjab University College of Pharmacy, University of the Punjab, Lahore 54000, PakistanInstitute of Industrial Biotechnology, Government College University, Lahore 54000, PakistanOffice of Research, Innovation and Commercialization, Lahore College for Women University, Lahore 54000, PakistanGulab Devi Institute of Pharmacy, Gulab Devi Educational Complex, Lahore 54000, PakistanFaculty of Medicine, Transilvania University of Brasov, 500036 Brasov, RomaniaFaculty of Medicine, University “Lucian Blaga” Sibiu, 550169 Sibiu, RomaniaHundreds of the plants have been explored and evaluated for antioxidant and anti-amnesic activities, so far. This study was designed to report the biomolecules of <i>Pimpinella anisum</i> L. for the said activities. The aqueous extract of dried <i>P. anisum</i> seeds was fractionated via column chromatography and the fractions so obtained were assessed for the inhibition of acetylcholinesterase (AChE) via in vitro analysis. The fraction which best inhibited AChE was so named as the <i>P. anisum</i> active fraction (P.aAF). The P.aAF was then chemically analyzed via GCMS, which indicated that oxadiazole compounds were present in it. The P.aAF was then administered to albino mice to conduct the in vivo (behavioral and biochemical) studies. The results of the behavioral studies indicated the significant (<i>p</i> < 0.001) increase in inflexion ratio, by the number of hole-pokings through holes and time spent in a dark area by P.aAF treated mice. Biochemical studies demonstrated that the oxadiazole present in P.aAF on one hand presented a noteworthy reduction in MDA and the AChE level and on the other hand promoted the levels of CAT, SOD and GSH in mice brain. The LD<sub>50</sub> for P.aAF was calculated as 95 mg/Kg/p.o. The findings thus supported that the antioxidant and anticholinesterase activities of <i>P. anisum</i> are due to its oxadiazole compounds.https://www.mdpi.com/1420-3049/28/5/2217dementiabrainapiolcholinergicnatural productsmemory |
| spellingShingle | Aamir Mushtaq Fatima Habib Rosana Manea Rukhsana Anwar Umar Farooq Gohar Muhammad Zia-Ul-Haq Mobasher Ahmad Claudia Mihaela Gavris Liana Chicea Biomolecular Screening of <i>Pimpinella anisum</i> L. for Antioxidant and Anticholinesterase Activity in Mice Brain Molecules dementia brain apiol cholinergic natural products memory |
| title | Biomolecular Screening of <i>Pimpinella anisum</i> L. for Antioxidant and Anticholinesterase Activity in Mice Brain |
| title_full | Biomolecular Screening of <i>Pimpinella anisum</i> L. for Antioxidant and Anticholinesterase Activity in Mice Brain |
| title_fullStr | Biomolecular Screening of <i>Pimpinella anisum</i> L. for Antioxidant and Anticholinesterase Activity in Mice Brain |
| title_full_unstemmed | Biomolecular Screening of <i>Pimpinella anisum</i> L. for Antioxidant and Anticholinesterase Activity in Mice Brain |
| title_short | Biomolecular Screening of <i>Pimpinella anisum</i> L. for Antioxidant and Anticholinesterase Activity in Mice Brain |
| title_sort | biomolecular screening of i pimpinella anisum i l for antioxidant and anticholinesterase activity in mice brain |
| topic | dementia brain apiol cholinergic natural products memory |
| url | https://www.mdpi.com/1420-3049/28/5/2217 |
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