Hyper-Branched Cationic Cyclodextrin Polymers for Improving Plasmid Transfection in 2D and 3D Spheroid Cells
In this article, we used monolayer two dimensional (2D) and 3D multicellular spheroid models to improve our understanding of the gene delivery process of a new modified cationic hyper-branched cyclodextrin-based polymer (Ppoly)-loaded plasmid encoding Enhanced Green Fluorescent Protein (EGFP). A com...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/12/2690 |
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| author | Yousef Khazaei Monfared Mohammad Mahmoudian Claudio Cecone Fabrizio Caldera Sanya Haiaty Hamid Reza Heidari Reza Rahbarghazi Adrián Matencio Parvin Zakeri-Milani Francesco Trotta |
| author_facet | Yousef Khazaei Monfared Mohammad Mahmoudian Claudio Cecone Fabrizio Caldera Sanya Haiaty Hamid Reza Heidari Reza Rahbarghazi Adrián Matencio Parvin Zakeri-Milani Francesco Trotta |
| author_sort | Yousef Khazaei Monfared |
| collection | DOAJ |
| description | In this article, we used monolayer two dimensional (2D) and 3D multicellular spheroid models to improve our understanding of the gene delivery process of a new modified cationic hyper-branched cyclodextrin-based polymer (Ppoly)-loaded plasmid encoding Enhanced Green Fluorescent Protein (EGFP). A comparison between the cytotoxicity effect and transfection efficiency of the plasmid DNA (pDNA)-loaded Ppoly system in 2D and 3D spheroid cells determined that the transfection efficiency and cytotoxicity of Ppoly–pDNA nanocomplexes were lower in 3D spheroids than in 2D monolayer cells. Furthermore, histopathology visualization of Ppoly–pDNA complex cellular uptake in 3D spheroids demonstrated that Ppoly penetrated into the inner layers. This study indicated that the Ppoly, as a non-viral gene delivery system in complex with pDNA, is hemocompatible, non-toxic, high in encapsulation efficiency, and has good transfection efficiency in both 2D and 3D cell cultures compared to free pDNA and lipofectamine (as the control). |
| first_indexed | 2024-03-09T15:58:20Z |
| format | Article |
| id | doaj.art-5d76deaad210416c9bea504603282622 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T15:58:20Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-5d76deaad210416c9bea5046032826222023-11-24T17:20:20ZengMDPI AGPharmaceutics1999-49232022-12-011412269010.3390/pharmaceutics14122690Hyper-Branched Cationic Cyclodextrin Polymers for Improving Plasmid Transfection in 2D and 3D Spheroid CellsYousef Khazaei Monfared0Mohammad Mahmoudian1Claudio Cecone2Fabrizio Caldera3Sanya Haiaty4Hamid Reza Heidari5Reza Rahbarghazi6Adrián Matencio7Parvin Zakeri-Milani8Francesco Trotta9Dipartimento Di Chimica and NIS, Università di Torino, Via P. Giuria 7, 10125 Torino, ItalyFaculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 5166-15731, IranDipartimento Di Chimica and NIS, Università di Torino, Via P. Giuria 7, 10125 Torino, ItalyDipartimento Di Chimica and NIS, Università di Torino, Via P. Giuria 7, 10125 Torino, ItalyInfectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz 5166-15731, IranDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 5166-15731, IranDepartment of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz 5166-15731, IranDipartimento Di Chimica and NIS, Università di Torino, Via P. Giuria 7, 10125 Torino, ItalyLiver and Gastrointestinal Diseases Research Centre, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 5166-15731, IranDipartimento Di Chimica and NIS, Università di Torino, Via P. Giuria 7, 10125 Torino, ItalyIn this article, we used monolayer two dimensional (2D) and 3D multicellular spheroid models to improve our understanding of the gene delivery process of a new modified cationic hyper-branched cyclodextrin-based polymer (Ppoly)-loaded plasmid encoding Enhanced Green Fluorescent Protein (EGFP). A comparison between the cytotoxicity effect and transfection efficiency of the plasmid DNA (pDNA)-loaded Ppoly system in 2D and 3D spheroid cells determined that the transfection efficiency and cytotoxicity of Ppoly–pDNA nanocomplexes were lower in 3D spheroids than in 2D monolayer cells. Furthermore, histopathology visualization of Ppoly–pDNA complex cellular uptake in 3D spheroids demonstrated that Ppoly penetrated into the inner layers. This study indicated that the Ppoly, as a non-viral gene delivery system in complex with pDNA, is hemocompatible, non-toxic, high in encapsulation efficiency, and has good transfection efficiency in both 2D and 3D cell cultures compared to free pDNA and lipofectamine (as the control).https://www.mdpi.com/1999-4923/14/12/2690cyclodextrin hyperbranched polymerstransfectionplasmid eGFPspheroid 3DDAPI |
| spellingShingle | Yousef Khazaei Monfared Mohammad Mahmoudian Claudio Cecone Fabrizio Caldera Sanya Haiaty Hamid Reza Heidari Reza Rahbarghazi Adrián Matencio Parvin Zakeri-Milani Francesco Trotta Hyper-Branched Cationic Cyclodextrin Polymers for Improving Plasmid Transfection in 2D and 3D Spheroid Cells Pharmaceutics cyclodextrin hyperbranched polymers transfection plasmid eGFP spheroid 3D DAPI |
| title | Hyper-Branched Cationic Cyclodextrin Polymers for Improving Plasmid Transfection in 2D and 3D Spheroid Cells |
| title_full | Hyper-Branched Cationic Cyclodextrin Polymers for Improving Plasmid Transfection in 2D and 3D Spheroid Cells |
| title_fullStr | Hyper-Branched Cationic Cyclodextrin Polymers for Improving Plasmid Transfection in 2D and 3D Spheroid Cells |
| title_full_unstemmed | Hyper-Branched Cationic Cyclodextrin Polymers for Improving Plasmid Transfection in 2D and 3D Spheroid Cells |
| title_short | Hyper-Branched Cationic Cyclodextrin Polymers for Improving Plasmid Transfection in 2D and 3D Spheroid Cells |
| title_sort | hyper branched cationic cyclodextrin polymers for improving plasmid transfection in 2d and 3d spheroid cells |
| topic | cyclodextrin hyperbranched polymers transfection plasmid eGFP spheroid 3D DAPI |
| url | https://www.mdpi.com/1999-4923/14/12/2690 |
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