Biologically Active Ajuga Species Extracts Modulate Supportive Processes for Cancer Cell Development
Backround:Ajuga species have been used in traditional medicine for their diuretic, anti-inflammatory, wound-healing, and hepatoprotective properties.Purpose: The phytochemical profile and anticancer potential of three Ajuga sp. (A. genevensis, A. chamaepitys, and A. laxmannii) from Romania was inves...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2019.00334/full |
| _version_ | 1818517123593404416 |
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| author | Valentin-Florian Rauca Valentin-Florian Rauca Laurian Vlase Tibor Casian Alina Sesarman Alina Sesarman Ana-Maria Gheldiu Andrei Mocan Manuela Banciu Manuela Banciu Anca Toiu |
| author_facet | Valentin-Florian Rauca Valentin-Florian Rauca Laurian Vlase Tibor Casian Alina Sesarman Alina Sesarman Ana-Maria Gheldiu Andrei Mocan Manuela Banciu Manuela Banciu Anca Toiu |
| author_sort | Valentin-Florian Rauca |
| collection | DOAJ |
| description | Backround:Ajuga species have been used in traditional medicine for their diuretic, anti-inflammatory, wound-healing, and hepatoprotective properties.Purpose: The phytochemical profile and anticancer potential of three Ajuga sp. (A. genevensis, A. chamaepitys, and A. laxmannii) from Romania was investigated.Materials and Methods: The phytochemicals were extracted from the aerial parts of Ajuga sp. by using different solvents and methods. The hydroalcoholic extracts were examined for total phenolic, flavonoid and iridoid contents, and HPLC/MS was used to analyze the polyphenolic compounds and iridoids. The phytochemical profile was also evaluated by principal component analysis in connection with antitumor efficacy of extracts. The antiproliferative potential was evaluated using the ELISA BrdU-colorimetric immunoassay. Western Blot with regard to inflammatory protein NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) p65 subunit expression in cell lysates was performed. Quantification of oxidative stress marker malondialdehyde (MDA) was determined by high-performance liquid chromatography (HPLC). Enzymatic and non-enzymatic antioxidant capability was assessed by measuring catalase activity and by evaluating the total antioxidant capacity (TAC) of treated cells.Results:Ajuga laxmannii ethanol extract showed the highest total phenolic and flavonoid content, while A. genevensis ethanol extract was more abundant in iridoids. The overall cytostatic effect of the investigated plant extracts was exerted through strong inhibitory actions on NF-κB, the key molecule involved in the inflammatory response and via oxidative stress modulatory effects in both murine colon carcinoma and melanoma cell lines.Conclusion:Ajuga laxmannii showed the most significant antitumor activity and represents an important source of bioactive compounds, possibly an additional form of treatment alongside conventional anticancer drugs. |
| first_indexed | 2024-12-11T00:51:57Z |
| format | Article |
| id | doaj.art-5d77a2fa99924cfdb2938d43e5db6b14 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-11T00:51:57Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-5d77a2fa99924cfdb2938d43e5db6b142022-12-22T01:26:36ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122019-04-011010.3389/fphar.2019.00334441152Biologically Active Ajuga Species Extracts Modulate Supportive Processes for Cancer Cell DevelopmentValentin-Florian Rauca0Valentin-Florian Rauca1Laurian Vlase2Tibor Casian3Alina Sesarman4Alina Sesarman5Ana-Maria Gheldiu6Andrei Mocan7Manuela Banciu8Manuela Banciu9Anca Toiu10Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babes-Bolyai University, Cluj-Napoca, RomaniaMolecular Biology Centre, Institute for Interdisciplinary Research in Bio-Nano-Sciences, Babes-Bolyai University, Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babes-Bolyai University, Cluj-Napoca, RomaniaMolecular Biology Centre, Institute for Interdisciplinary Research in Bio-Nano-Sciences, Babes-Bolyai University, Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babes-Bolyai University, Cluj-Napoca, RomaniaMolecular Biology Centre, Institute for Interdisciplinary Research in Bio-Nano-Sciences, Babes-Bolyai University, Cluj-Napoca, RomaniaDepartment of Pharmacognosy, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaBackround:Ajuga species have been used in traditional medicine for their diuretic, anti-inflammatory, wound-healing, and hepatoprotective properties.Purpose: The phytochemical profile and anticancer potential of three Ajuga sp. (A. genevensis, A. chamaepitys, and A. laxmannii) from Romania was investigated.Materials and Methods: The phytochemicals were extracted from the aerial parts of Ajuga sp. by using different solvents and methods. The hydroalcoholic extracts were examined for total phenolic, flavonoid and iridoid contents, and HPLC/MS was used to analyze the polyphenolic compounds and iridoids. The phytochemical profile was also evaluated by principal component analysis in connection with antitumor efficacy of extracts. The antiproliferative potential was evaluated using the ELISA BrdU-colorimetric immunoassay. Western Blot with regard to inflammatory protein NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) p65 subunit expression in cell lysates was performed. Quantification of oxidative stress marker malondialdehyde (MDA) was determined by high-performance liquid chromatography (HPLC). Enzymatic and non-enzymatic antioxidant capability was assessed by measuring catalase activity and by evaluating the total antioxidant capacity (TAC) of treated cells.Results:Ajuga laxmannii ethanol extract showed the highest total phenolic and flavonoid content, while A. genevensis ethanol extract was more abundant in iridoids. The overall cytostatic effect of the investigated plant extracts was exerted through strong inhibitory actions on NF-κB, the key molecule involved in the inflammatory response and via oxidative stress modulatory effects in both murine colon carcinoma and melanoma cell lines.Conclusion:Ajuga laxmannii showed the most significant antitumor activity and represents an important source of bioactive compounds, possibly an additional form of treatment alongside conventional anticancer drugs.https://www.frontiersin.org/article/10.3389/fphar.2019.00334/fullAjuga speciespolyphenolsiridoidsantiproliferative activitynuclear factor – kappa Boxidative stress |
| spellingShingle | Valentin-Florian Rauca Valentin-Florian Rauca Laurian Vlase Tibor Casian Alina Sesarman Alina Sesarman Ana-Maria Gheldiu Andrei Mocan Manuela Banciu Manuela Banciu Anca Toiu Biologically Active Ajuga Species Extracts Modulate Supportive Processes for Cancer Cell Development Frontiers in Pharmacology Ajuga species polyphenols iridoids antiproliferative activity nuclear factor – kappa B oxidative stress |
| title | Biologically Active Ajuga Species Extracts Modulate Supportive Processes for Cancer Cell Development |
| title_full | Biologically Active Ajuga Species Extracts Modulate Supportive Processes for Cancer Cell Development |
| title_fullStr | Biologically Active Ajuga Species Extracts Modulate Supportive Processes for Cancer Cell Development |
| title_full_unstemmed | Biologically Active Ajuga Species Extracts Modulate Supportive Processes for Cancer Cell Development |
| title_short | Biologically Active Ajuga Species Extracts Modulate Supportive Processes for Cancer Cell Development |
| title_sort | biologically active ajuga species extracts modulate supportive processes for cancer cell development |
| topic | Ajuga species polyphenols iridoids antiproliferative activity nuclear factor – kappa B oxidative stress |
| url | https://www.frontiersin.org/article/10.3389/fphar.2019.00334/full |
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