Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model
Non-typeable (NT) <i>Staphylococcus aureus</i> strains are associated with chronic bovine mastitis. This study investigates the impact of biofilm formation by clinical NT <i>S. aureus</i> on cytokine production and mammary tissue damage by using a mouse mastitis model. Mice i...
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MDPI AG
2021-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/11/3047 |
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| author | Jully Gogoi-Tiwari Dorji Dorji Harish Kumar Tiwari Gayatri Shirolkar Joshua W. Aleri Trilochan Mukkur |
| author_facet | Jully Gogoi-Tiwari Dorji Dorji Harish Kumar Tiwari Gayatri Shirolkar Joshua W. Aleri Trilochan Mukkur |
| author_sort | Jully Gogoi-Tiwari |
| collection | DOAJ |
| description | Non-typeable (NT) <i>Staphylococcus aureus</i> strains are associated with chronic bovine mastitis. This study investigates the impact of biofilm formation by clinical NT <i>S. aureus</i> on cytokine production and mammary tissue damage by using a mouse mastitis model. Mice infected with two different NT <i>S. aureus</i> strains with strong and weak biofilm forming potential demonstrated identical clinical symptoms (moderate), minimal inflammatory infiltrates, and tissue damage (level 1 histopathological changes) in the mammary glands. However, the <i>S. aureus</i> load in the mammary glands of mice and the level of pro-inflammatory cytokines (IL-1β, IL-6, IL-12, IL-17 and IFN-γ) in serum were significantly higher (<i>p</i> ≤ 0.05) in those infected with the strong biofilm forming NT <i>S. aureus</i> strain. The level of IL-6 in sera samples of these mice was extremely high (15,479.9 ± 532 Pg/mL). Furthermore, these mice died in 24h of post infection compared to 30 h in the weak biofilm forming NT <i>S. aureus</i> infected group. The study demonstrates no association between the strength of PIA (polysaccharide intercellular adhesion)-dependent biofilm production by clinical NT <i>S. aureus</i> and mammary gland pathology in a mouse mastitis model. However, the role of biofilm in the virulence of <i>S. aureus</i> advancing the time of mortality in mice warrants further investigation. |
| first_indexed | 2024-03-09T04:36:49Z |
| format | Article |
| id | doaj.art-5d784ff5d5f84d69a60ce78260028fef |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T04:36:49Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-5d784ff5d5f84d69a60ce78260028fef2023-12-03T13:26:50ZengMDPI AGAnimals2076-26152021-10-011111304710.3390/ani11113047Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis ModelJully Gogoi-Tiwari0Dorji Dorji1Harish Kumar Tiwari2Gayatri Shirolkar3Joshua W. Aleri4Trilochan Mukkur5Curtin Health Innovation Research Institute, Curtin Medical School, Curtin University, Bentley, Perth, WA 6102, AustraliaCurtin Health Innovation Research Institute, Curtin Medical School, Curtin University, Bentley, Perth, WA 6102, AustraliaAsia-Pacific Consortium of Veterinary Epidemiology, Sydney School of Veterinary Science, The University of Sydney, Camden, NSW 2570, AustraliaCurtin Health Innovation Research Institute, Curtin Medical School, Curtin University, Bentley, Perth, WA 6102, AustraliaSchool of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, WA 6150, AustraliaCurtin Health Innovation Research Institute, Curtin Medical School, Curtin University, Bentley, Perth, WA 6102, AustraliaNon-typeable (NT) <i>Staphylococcus aureus</i> strains are associated with chronic bovine mastitis. This study investigates the impact of biofilm formation by clinical NT <i>S. aureus</i> on cytokine production and mammary tissue damage by using a mouse mastitis model. Mice infected with two different NT <i>S. aureus</i> strains with strong and weak biofilm forming potential demonstrated identical clinical symptoms (moderate), minimal inflammatory infiltrates, and tissue damage (level 1 histopathological changes) in the mammary glands. However, the <i>S. aureus</i> load in the mammary glands of mice and the level of pro-inflammatory cytokines (IL-1β, IL-6, IL-12, IL-17 and IFN-γ) in serum were significantly higher (<i>p</i> ≤ 0.05) in those infected with the strong biofilm forming NT <i>S. aureus</i> strain. The level of IL-6 in sera samples of these mice was extremely high (15,479.9 ± 532 Pg/mL). Furthermore, these mice died in 24h of post infection compared to 30 h in the weak biofilm forming NT <i>S. aureus</i> infected group. The study demonstrates no association between the strength of PIA (polysaccharide intercellular adhesion)-dependent biofilm production by clinical NT <i>S. aureus</i> and mammary gland pathology in a mouse mastitis model. However, the role of biofilm in the virulence of <i>S. aureus</i> advancing the time of mortality in mice warrants further investigation.https://www.mdpi.com/2076-2615/11/11/3047<i>Staphylococcus aureus</i>non-typeablebiofilmtissue damageinflammationbovine mastitis |
| spellingShingle | Jully Gogoi-Tiwari Dorji Dorji Harish Kumar Tiwari Gayatri Shirolkar Joshua W. Aleri Trilochan Mukkur Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model Animals <i>Staphylococcus aureus</i> non-typeable biofilm tissue damage inflammation bovine mastitis |
| title | Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model |
| title_full | Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model |
| title_fullStr | Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model |
| title_full_unstemmed | Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model |
| title_short | Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model |
| title_sort | phenotypic pia dependent biofilm production by clinical non typeable i staphylococcus aureus i is not associated with the intensity of inflammation in mammary gland a pilot study using mouse mastitis model |
| topic | <i>Staphylococcus aureus</i> non-typeable biofilm tissue damage inflammation bovine mastitis |
| url | https://www.mdpi.com/2076-2615/11/11/3047 |
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