Circulating Extracellular-Vesicle-Incorporated MicroRNAs as Potential Biomarkers for Ischemic Stroke in Patients With Cancer
Background and Purpose This study aimed to evaluate whether extracellular-vesicle-incorporated microRNAs (miRNAs) are potential biomarkers for cancer-related stroke. Methods This cohort study compared patients with active cancer who had embolic stroke of unknown sources (cancer-stroke group) with pa...
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| Format: | Article |
| Language: | English |
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Korean Stroke Society
2023-05-01
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| Series: | Journal of Stroke |
| Subjects: | |
| Online Access: | http://www.j-stroke.org/upload/pdf/jos-2022-02327.pdf |
| _version_ | 1797653484740280320 |
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| author | Oh Young Bang Eun Hee Kim Mi Jeong Oh Jaein Yoo Gyun Sik Oh Jong-Won Chung Woo-Keun Seo Gyeong-Moon Kim Myung-Ju Ahn Seong Wook Yang |
| author_facet | Oh Young Bang Eun Hee Kim Mi Jeong Oh Jaein Yoo Gyun Sik Oh Jong-Won Chung Woo-Keun Seo Gyeong-Moon Kim Myung-Ju Ahn Seong Wook Yang |
| author_sort | Oh Young Bang |
| collection | DOAJ |
| description | Background and Purpose This study aimed to evaluate whether extracellular-vesicle-incorporated microRNAs (miRNAs) are potential biomarkers for cancer-related stroke. Methods This cohort study compared patients with active cancer who had embolic stroke of unknown sources (cancer-stroke group) with patients with only cancer, patients with only stroke, and healthy individuals (control groups). The expression profiles of miRNAs encapsulated in plasma exosomes and microvesicles were evaluated using microarray and validated using quantitative real-time polymerase chain reaction. The XENO-QTM miRNA assay technology was used to determine the absolute copy numbers of individual miRNAs in an external validation cohort. Results This study recruited 220 patients, of which 45 had cancer-stroke, 76 were healthy controls, 39 were cancer controls, and 60 were stroke controls. Three miRNAs (miR-205-5p, miR-645, and miR-646) were specifically incorporated into microvesicles in patients with cancer-related stroke, cancer controls, and stroke controls. The area under the receiver operating characteristic curves of these three miRNAs were 0.7692–0.8510 for the differentiation of patients with cancer-stroke from cancer-controls and 0.8077–0.8846 for the differentiation of patients with cancer-stroke from stroke controls. The levels of several miRNAs were elevated in the plasma exosomes of patients with cancer, but were lower than those in plasma microvesicles. An in vivo study showed that systemic injection of miR-205-5p promoted the development of arterial thrombosis and elevation of D-dimer levels Conclusion Stroke due to cancer-related coagulopathy was associated with deregulated expression of miRNAs, particularly microvesicle-incorporated miR-205-5p, miR-645, and miR-646. Further prospective studies of extracellular-vesicle-incorporated miRNAs are required to confirm the diagnostic role of miRNAs in patients with stroke and to screen the roles of miRNAs in patients with cancer. |
| first_indexed | 2024-03-11T16:45:14Z |
| format | Article |
| id | doaj.art-5d7fb4a72a3e4b26a91b945914b65de4 |
| institution | Directory Open Access Journal |
| issn | 2287-6391 2287-6405 |
| language | English |
| last_indexed | 2024-03-11T16:45:14Z |
| publishDate | 2023-05-01 |
| publisher | Korean Stroke Society |
| record_format | Article |
| series | Journal of Stroke |
| spelling | doaj.art-5d7fb4a72a3e4b26a91b945914b65de42023-10-23T00:17:46ZengKorean Stroke SocietyJournal of Stroke2287-63912287-64052023-05-0125225126510.5853/jos.2022.02327481Circulating Extracellular-Vesicle-Incorporated MicroRNAs as Potential Biomarkers for Ischemic Stroke in Patients With CancerOh Young Bang0Eun Hee Kim1Mi Jeong Oh2Jaein Yoo3Gyun Sik Oh4Jong-Won Chung5Woo-Keun Seo6Gyeong-Moon Kim7Myung-Ju Ahn8Seong Wook Yang9 Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea S&E bio Co., Ltd., Seoul, Korea Translational and Stem Cell Research Laboratory on Stroke, Samsung Medical Center, Seoul, Korea Translational and Stem Cell Research Laboratory on Stroke, Samsung Medical Center, Seoul, Korea S&E bio Co., Ltd., Seoul, Korea Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Department of Hemato-Oncology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, KoreaBackground and Purpose This study aimed to evaluate whether extracellular-vesicle-incorporated microRNAs (miRNAs) are potential biomarkers for cancer-related stroke. Methods This cohort study compared patients with active cancer who had embolic stroke of unknown sources (cancer-stroke group) with patients with only cancer, patients with only stroke, and healthy individuals (control groups). The expression profiles of miRNAs encapsulated in plasma exosomes and microvesicles were evaluated using microarray and validated using quantitative real-time polymerase chain reaction. The XENO-QTM miRNA assay technology was used to determine the absolute copy numbers of individual miRNAs in an external validation cohort. Results This study recruited 220 patients, of which 45 had cancer-stroke, 76 were healthy controls, 39 were cancer controls, and 60 were stroke controls. Three miRNAs (miR-205-5p, miR-645, and miR-646) were specifically incorporated into microvesicles in patients with cancer-related stroke, cancer controls, and stroke controls. The area under the receiver operating characteristic curves of these three miRNAs were 0.7692–0.8510 for the differentiation of patients with cancer-stroke from cancer-controls and 0.8077–0.8846 for the differentiation of patients with cancer-stroke from stroke controls. The levels of several miRNAs were elevated in the plasma exosomes of patients with cancer, but were lower than those in plasma microvesicles. An in vivo study showed that systemic injection of miR-205-5p promoted the development of arterial thrombosis and elevation of D-dimer levels Conclusion Stroke due to cancer-related coagulopathy was associated with deregulated expression of miRNAs, particularly microvesicle-incorporated miR-205-5p, miR-645, and miR-646. Further prospective studies of extracellular-vesicle-incorporated miRNAs are required to confirm the diagnostic role of miRNAs in patients with stroke and to screen the roles of miRNAs in patients with cancer.http://www.j-stroke.org/upload/pdf/jos-2022-02327.pdfcancerstrokecoagulopathybiomarkerextracellular vesiclemicrorna |
| spellingShingle | Oh Young Bang Eun Hee Kim Mi Jeong Oh Jaein Yoo Gyun Sik Oh Jong-Won Chung Woo-Keun Seo Gyeong-Moon Kim Myung-Ju Ahn Seong Wook Yang Circulating Extracellular-Vesicle-Incorporated MicroRNAs as Potential Biomarkers for Ischemic Stroke in Patients With Cancer Journal of Stroke cancer stroke coagulopathy biomarker extracellular vesicle microrna |
| title | Circulating Extracellular-Vesicle-Incorporated MicroRNAs as Potential Biomarkers for Ischemic Stroke in Patients With Cancer |
| title_full | Circulating Extracellular-Vesicle-Incorporated MicroRNAs as Potential Biomarkers for Ischemic Stroke in Patients With Cancer |
| title_fullStr | Circulating Extracellular-Vesicle-Incorporated MicroRNAs as Potential Biomarkers for Ischemic Stroke in Patients With Cancer |
| title_full_unstemmed | Circulating Extracellular-Vesicle-Incorporated MicroRNAs as Potential Biomarkers for Ischemic Stroke in Patients With Cancer |
| title_short | Circulating Extracellular-Vesicle-Incorporated MicroRNAs as Potential Biomarkers for Ischemic Stroke in Patients With Cancer |
| title_sort | circulating extracellular vesicle incorporated micrornas as potential biomarkers for ischemic stroke in patients with cancer |
| topic | cancer stroke coagulopathy biomarker extracellular vesicle microrna |
| url | http://www.j-stroke.org/upload/pdf/jos-2022-02327.pdf |
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