First study of in vitro protective effect of Lepidium meyenii (Maca) on frozen–thawed bovine spermatozoa
Background and Aim: Lepidium meyenii Walp (Maca) is an herbaceous plant that grows in the Peruvian Andes and it has been widely used as a nutritional supplement and fertility enhancer and has been used in the treatment of a variety of diseases, such as rheumatism, respiratory disorders, and anemia....
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| Format: | Article |
| Language: | English |
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Veterinary World
2022-06-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.15/June-2022/10.pdf |
| _version_ | 1818470572714098688 |
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| author | Johanna Leiva-Revilla Miriam Rolón Abolghasem Siyadatpanah Maria de Lourdes Pereira Veeranoot Nissapatorn |
| author_facet | Johanna Leiva-Revilla Miriam Rolón Abolghasem Siyadatpanah Maria de Lourdes Pereira Veeranoot Nissapatorn |
| author_sort | Johanna Leiva-Revilla |
| collection | DOAJ |
| description | Background and Aim: Lepidium meyenii Walp (Maca) is an herbaceous plant that grows in the Peruvian Andes and it has been widely used as a nutritional supplement and fertility enhancer and has been used in the treatment of a variety of diseases, such as rheumatism, respiratory disorders, and anemia. The most notable feature of Maca is its potent antioxidant capacity, which helps in the scavenging of free radicals and protection of cells from oxidative stress. This study aimed to evaluate the in vitro effect of Maca extract on thawed sperm cells from bulls.
Materials and Methods: Three dilutions of 1, 10, and 100 μg/mL of Maca extract were incubated with frozen–thawed bovine semen and analyzed at 1, 3, and 24 h of exposure time, evaluating the activity of the extract on the DNA, motility, morphology, viability, integrity of the membrane and acrosome of spermatozoa.
Results: The Maca extract improved the studied sperm parameters of motility, acrosome integrity, vitality, and DNA integrity of sperm cells at a concentration of 10 –g/mL, and at 1 –g/mL, an improvement was observed in the morphology and integrity of the membrane. However, the best activity of the Maca extract was observed on the DNA integrity of the sperm, which was effective at the three concentrations evaluated after 24 h of incubation.
Conclusion: The results indicate that L. meyenii can help in maintaining spermatozoa cellular integrity after the frozen–thaw process, especially in the protection against DNA fragmentation. Therefore, Maca would be a feasible supplementation to protect sperm to maintain their fertile ability after thawing. |
| first_indexed | 2024-04-13T21:39:00Z |
| format | Article |
| id | doaj.art-5d82c4d6b1c74ead9c4793fcde988c86 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-13T21:39:00Z |
| publishDate | 2022-06-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-5d82c4d6b1c74ead9c4793fcde988c862022-12-22T02:28:50ZengVeterinary WorldVeterinary World0972-89882231-09162022-06-011561481148810.14202/vetworld.2022.1481-1488First study of in vitro protective effect of Lepidium meyenii (Maca) on frozen–thawed bovine spermatozoaJohanna Leiva-Revilla0https://orcid.org/0000-0001-8756-6338Miriam Rolón1https://orcid.org/0000-0001-8123-4260Abolghasem Siyadatpanah2https://orcid.org/0000-0003-3172-5939Maria de Lourdes Pereira3https://orcid.org/0000-0001-6703-7869Veeranoot Nissapatorn4https://orcid.org/0000-0001-8652-7556Center for the Development of Scientific Research (CEDIC), Manduvira 635, CP 1255, Asunción, Paraguay.Center for the Development of Scientific Research (CEDIC), Manduvira 635, CP 1255, Asunción, Paraguay.Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand, Iran.CICECO-Aveiro Institute of Materials and Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, Portugal.School of Allied Health Sciences, World Union for Herbal Drug Discovery, and Research Excellence Center for Innovation and Health Products, Walailak University, Nakhon Si Thammarat 80160, Thailand.Background and Aim: Lepidium meyenii Walp (Maca) is an herbaceous plant that grows in the Peruvian Andes and it has been widely used as a nutritional supplement and fertility enhancer and has been used in the treatment of a variety of diseases, such as rheumatism, respiratory disorders, and anemia. The most notable feature of Maca is its potent antioxidant capacity, which helps in the scavenging of free radicals and protection of cells from oxidative stress. This study aimed to evaluate the in vitro effect of Maca extract on thawed sperm cells from bulls. Materials and Methods: Three dilutions of 1, 10, and 100 μg/mL of Maca extract were incubated with frozen–thawed bovine semen and analyzed at 1, 3, and 24 h of exposure time, evaluating the activity of the extract on the DNA, motility, morphology, viability, integrity of the membrane and acrosome of spermatozoa. Results: The Maca extract improved the studied sperm parameters of motility, acrosome integrity, vitality, and DNA integrity of sperm cells at a concentration of 10 –g/mL, and at 1 –g/mL, an improvement was observed in the morphology and integrity of the membrane. However, the best activity of the Maca extract was observed on the DNA integrity of the sperm, which was effective at the three concentrations evaluated after 24 h of incubation. Conclusion: The results indicate that L. meyenii can help in maintaining spermatozoa cellular integrity after the frozen–thaw process, especially in the protection against DNA fragmentation. Therefore, Maca would be a feasible supplementation to protect sperm to maintain their fertile ability after thawing.http://www.veterinaryworld.org/Vol.15/June-2022/10.pdfaberdeenangusantioxidantbullfrozen-thawed semenlepidium meyenii (maca)reproductive health |
| spellingShingle | Johanna Leiva-Revilla Miriam Rolón Abolghasem Siyadatpanah Maria de Lourdes Pereira Veeranoot Nissapatorn First study of in vitro protective effect of Lepidium meyenii (Maca) on frozen–thawed bovine spermatozoa Veterinary World aberdeenangus antioxidant bull frozen-thawed semen lepidium meyenii (maca) reproductive health |
| title | First study of in vitro protective effect of Lepidium meyenii (Maca) on frozen–thawed bovine spermatozoa |
| title_full | First study of in vitro protective effect of Lepidium meyenii (Maca) on frozen–thawed bovine spermatozoa |
| title_fullStr | First study of in vitro protective effect of Lepidium meyenii (Maca) on frozen–thawed bovine spermatozoa |
| title_full_unstemmed | First study of in vitro protective effect of Lepidium meyenii (Maca) on frozen–thawed bovine spermatozoa |
| title_short | First study of in vitro protective effect of Lepidium meyenii (Maca) on frozen–thawed bovine spermatozoa |
| title_sort | first study of in vitro protective effect of lepidium meyenii maca on frozen thawed bovine spermatozoa |
| topic | aberdeenangus antioxidant bull frozen-thawed semen lepidium meyenii (maca) reproductive health |
| url | http://www.veterinaryworld.org/Vol.15/June-2022/10.pdf |
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