Determination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of <i>Sanghuangporus baumii</i> (Pilát) L.W. Zhou and Y.C. Dai
<i>Sanghuangporus</i>, a medicinal mushroom, has gained significant attention due to its beneficial properties. Phenolic acids are among the major bioactive compounds in <i>Sanghuangporus</i>, known for their antioxidant and anti-inflammatory activities. To precisely quantify...
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MDPI AG
2023-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/20/3565 |
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| author | Zhongjing Zhou Shuang Liang Xiaowei Zou Yi Teng Weike Wang Lizhong Fu |
| author_facet | Zhongjing Zhou Shuang Liang Xiaowei Zou Yi Teng Weike Wang Lizhong Fu |
| author_sort | Zhongjing Zhou |
| collection | DOAJ |
| description | <i>Sanghuangporus</i>, a medicinal mushroom, has gained significant attention due to its beneficial properties. Phenolic acids are among the major bioactive compounds in <i>Sanghuangporus</i>, known for their antioxidant and anti-inflammatory activities. To precisely quantify the phenolic acid content, we developed a method utilizing ultra-high-performance liquid chromatography with triple quadrupole (UHPLC-QqQ). This study optimized the UHPLC-QqQ conditions to simultaneously separate and detect eight phenolic acids in <i>Sanghuangporus baumii</i> (Pilát) L.W. Zhou and Y.C. Dai, including chlorogenic acid, <i>p</i>-coumaric acid, caffeic acid, cryptochlorogenic acid, protocatechuic acid, ferulic acid, sinapic acid, and syringic acid. The separation process utilized a ZORBAX Eclipse Plus C18 column using 0.01% formic acid and 2 mmol/L ammonium formate in water as the aqueous phase and methanol containing 0.01% formic acid and 2 mmol/L ammonium formate as the organic phase. Calibration curves were constructed using standard solutions to quantitatively determine the phenolic acid content. The results showed significant variation in phenolic acid content among <i>S. baumii</i> fruiting bodies, with Protocatechuic acid, <i>p</i>-coumaric acid, and caffeic acid being the most abundant. This method is valuable for quantifying phenolic acid compounds under different cultivation conditions. It provides excellent sensitivity, selectivity, and reproducibility for the quantification of phenolic acids in <i>Sanghuangporus</i>, contributing to a better understanding of its chemical composition and potential health benefits. This approach represents a novel technical means for the simultaneous analysis of compound phenolic acids in <i>Sanghuangporus</i> fruiting bodies. |
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| language | English |
| last_indexed | 2024-03-10T20:57:33Z |
| publishDate | 2023-10-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-5d85c408de14466bb1f990693e5011702023-11-19T17:48:49ZengMDPI AGPlants2223-77472023-10-011220356510.3390/plants12203565Determination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of <i>Sanghuangporus baumii</i> (Pilát) L.W. Zhou and Y.C. DaiZhongjing Zhou0Shuang Liang1Xiaowei Zou2Yi Teng3Weike Wang4Lizhong Fu5State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaCollege of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 311402, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaHangzhou Academy of Agricultural Sciences, Hangzhou 310024, ChinaCollege of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 311402, China<i>Sanghuangporus</i>, a medicinal mushroom, has gained significant attention due to its beneficial properties. Phenolic acids are among the major bioactive compounds in <i>Sanghuangporus</i>, known for their antioxidant and anti-inflammatory activities. To precisely quantify the phenolic acid content, we developed a method utilizing ultra-high-performance liquid chromatography with triple quadrupole (UHPLC-QqQ). This study optimized the UHPLC-QqQ conditions to simultaneously separate and detect eight phenolic acids in <i>Sanghuangporus baumii</i> (Pilát) L.W. Zhou and Y.C. Dai, including chlorogenic acid, <i>p</i>-coumaric acid, caffeic acid, cryptochlorogenic acid, protocatechuic acid, ferulic acid, sinapic acid, and syringic acid. The separation process utilized a ZORBAX Eclipse Plus C18 column using 0.01% formic acid and 2 mmol/L ammonium formate in water as the aqueous phase and methanol containing 0.01% formic acid and 2 mmol/L ammonium formate as the organic phase. Calibration curves were constructed using standard solutions to quantitatively determine the phenolic acid content. The results showed significant variation in phenolic acid content among <i>S. baumii</i> fruiting bodies, with Protocatechuic acid, <i>p</i>-coumaric acid, and caffeic acid being the most abundant. This method is valuable for quantifying phenolic acid compounds under different cultivation conditions. It provides excellent sensitivity, selectivity, and reproducibility for the quantification of phenolic acids in <i>Sanghuangporus</i>, contributing to a better understanding of its chemical composition and potential health benefits. This approach represents a novel technical means for the simultaneous analysis of compound phenolic acids in <i>Sanghuangporus</i> fruiting bodies.https://www.mdpi.com/2223-7747/12/20/3565<i>Sanghuangporus</i><i>Sanghuangporus baumii</i>phenolic acidUHPLC-QqQquantitative |
| spellingShingle | Zhongjing Zhou Shuang Liang Xiaowei Zou Yi Teng Weike Wang Lizhong Fu Determination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of <i>Sanghuangporus baumii</i> (Pilát) L.W. Zhou and Y.C. Dai Plants <i>Sanghuangporus</i> <i>Sanghuangporus baumii</i> phenolic acid UHPLC-QqQ quantitative |
| title | Determination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of <i>Sanghuangporus baumii</i> (Pilát) L.W. Zhou and Y.C. Dai |
| title_full | Determination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of <i>Sanghuangporus baumii</i> (Pilát) L.W. Zhou and Y.C. Dai |
| title_fullStr | Determination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of <i>Sanghuangporus baumii</i> (Pilát) L.W. Zhou and Y.C. Dai |
| title_full_unstemmed | Determination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of <i>Sanghuangporus baumii</i> (Pilát) L.W. Zhou and Y.C. Dai |
| title_short | Determination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of <i>Sanghuangporus baumii</i> (Pilát) L.W. Zhou and Y.C. Dai |
| title_sort | determination of phenolic acids using ultra high performance liquid chromatography coupled with triple quadrupole uhplc qqq in fruiting bodies of i sanghuangporus baumii i pilat l w zhou and y c dai |
| topic | <i>Sanghuangporus</i> <i>Sanghuangporus baumii</i> phenolic acid UHPLC-QqQ quantitative |
| url | https://www.mdpi.com/2223-7747/12/20/3565 |
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