Antioxidant Resveratrol Increases Lipolytic and Reduces Lipogenic Gene Expression under In Vitro Heat Stress Conditions in Dedifferentiated Adipocyte-Derived Progeny Cells from Dairy Cows
Heat stress (HS) induces oxidative stress by increasing reactive oxygen species (ROS), and the polyphenol resveratrol (RSV) has been shown to have antioxidant properties by reducing ROS. Hence, we aimed to examine the effects of RSV, HS and their interaction on bovine adipocytes. We generated bovine...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/6/905 |
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| author | Gitit Kra Jayasimha Rayalu Daddam Hadar Gabay Sara Yosefi Maya Zachut |
| author_facet | Gitit Kra Jayasimha Rayalu Daddam Hadar Gabay Sara Yosefi Maya Zachut |
| author_sort | Gitit Kra |
| collection | DOAJ |
| description | Heat stress (HS) induces oxidative stress by increasing reactive oxygen species (ROS), and the polyphenol resveratrol (RSV) has been shown to have antioxidant properties by reducing ROS. Hence, we aimed to examine the effects of RSV, HS and their interaction on bovine adipocytes. We generated bovine dedifferentiated adipocyte-derived progeny (DFAT) cells from subcutaneous adipose tissue and examined the effects of RSV (100 µM), heat conditions: isothermal (ISO-37 °C), short heat (SH-41.2 °C for 1 h) and long HS (LH-41.2 °C for 16 h), and their interaction on gene expression in DFAT-cells. In medium of DFAT-cells treated with RSV, malondialdehyde levels were reduced and oxygen-radical absorbance-capacity levels were increased compared to control. Treating DFAT-cells with RSV increased the relative mRNA expression of stress-induced-phosphoprotein-1 (<i>STIP1</i>) and the expression of hormone-sensitive-lipase (<i>LIPE</i>) and perilipin-1 (<i>PLIN1</i>), whereas it reduced the expressions of fatty-acid-synthase (<i>FASN</i>) and of pro-inflammatory chemotactic-C-C-motif-chemokine-ligand-2 (<i>CCL2</i>) also under HS. Moreover, reduced protein abundance of <i>FASN</i> was found in RSV-treated DFAT-cells compared to controls. Molecular docking of RSV with <i>FASN</i> confirmed its possible binding to <i>FASN</i> active site. This work demonstrates that RSV has an antioxidant effect on bovine DFAT cells and may induce adipose lipolysis and reduce lipogenesis also under in vitro HS conditions. |
| first_indexed | 2024-03-10T10:44:49Z |
| format | Article |
| id | doaj.art-5d8689cfe136426890059103be4e1784 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T10:44:49Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-5d8689cfe136426890059103be4e17842023-11-21T22:40:18ZengMDPI AGAntioxidants2076-39212021-06-0110690510.3390/antiox10060905Antioxidant Resveratrol Increases Lipolytic and Reduces Lipogenic Gene Expression under In Vitro Heat Stress Conditions in Dedifferentiated Adipocyte-Derived Progeny Cells from Dairy CowsGitit Kra0Jayasimha Rayalu Daddam1Hadar Gabay2Sara Yosefi3Maya Zachut4Volcani Center, Department of Ruminant Science, Institute of Animal Sciences, Agriculture Research Organization, Rishon Lezion 7505101, IsraelVolcani Center, Department of Ruminant Science, Institute of Animal Sciences, Agriculture Research Organization, Rishon Lezion 7505101, IsraelVolcani Center, Department of Ruminant Science, Institute of Animal Sciences, Agriculture Research Organization, Rishon Lezion 7505101, IsraelVolcani Center, Department of Poultry Science, Institute of Animal Sciences, Agriculture Research Organization, Rishon Lezion 7505101, IsraelVolcani Center, Department of Ruminant Science, Institute of Animal Sciences, Agriculture Research Organization, Rishon Lezion 7505101, IsraelHeat stress (HS) induces oxidative stress by increasing reactive oxygen species (ROS), and the polyphenol resveratrol (RSV) has been shown to have antioxidant properties by reducing ROS. Hence, we aimed to examine the effects of RSV, HS and their interaction on bovine adipocytes. We generated bovine dedifferentiated adipocyte-derived progeny (DFAT) cells from subcutaneous adipose tissue and examined the effects of RSV (100 µM), heat conditions: isothermal (ISO-37 °C), short heat (SH-41.2 °C for 1 h) and long HS (LH-41.2 °C for 16 h), and their interaction on gene expression in DFAT-cells. In medium of DFAT-cells treated with RSV, malondialdehyde levels were reduced and oxygen-radical absorbance-capacity levels were increased compared to control. Treating DFAT-cells with RSV increased the relative mRNA expression of stress-induced-phosphoprotein-1 (<i>STIP1</i>) and the expression of hormone-sensitive-lipase (<i>LIPE</i>) and perilipin-1 (<i>PLIN1</i>), whereas it reduced the expressions of fatty-acid-synthase (<i>FASN</i>) and of pro-inflammatory chemotactic-C-C-motif-chemokine-ligand-2 (<i>CCL2</i>) also under HS. Moreover, reduced protein abundance of <i>FASN</i> was found in RSV-treated DFAT-cells compared to controls. Molecular docking of RSV with <i>FASN</i> confirmed its possible binding to <i>FASN</i> active site. This work demonstrates that RSV has an antioxidant effect on bovine DFAT cells and may induce adipose lipolysis and reduce lipogenesis also under in vitro HS conditions.https://www.mdpi.com/2076-3921/10/6/905antioxidantDFAT cellsdocking<i>FASN</i>heat stressresveratrol |
| spellingShingle | Gitit Kra Jayasimha Rayalu Daddam Hadar Gabay Sara Yosefi Maya Zachut Antioxidant Resveratrol Increases Lipolytic and Reduces Lipogenic Gene Expression under In Vitro Heat Stress Conditions in Dedifferentiated Adipocyte-Derived Progeny Cells from Dairy Cows Antioxidants antioxidant DFAT cells docking <i>FASN</i> heat stress resveratrol |
| title | Antioxidant Resveratrol Increases Lipolytic and Reduces Lipogenic Gene Expression under In Vitro Heat Stress Conditions in Dedifferentiated Adipocyte-Derived Progeny Cells from Dairy Cows |
| title_full | Antioxidant Resveratrol Increases Lipolytic and Reduces Lipogenic Gene Expression under In Vitro Heat Stress Conditions in Dedifferentiated Adipocyte-Derived Progeny Cells from Dairy Cows |
| title_fullStr | Antioxidant Resveratrol Increases Lipolytic and Reduces Lipogenic Gene Expression under In Vitro Heat Stress Conditions in Dedifferentiated Adipocyte-Derived Progeny Cells from Dairy Cows |
| title_full_unstemmed | Antioxidant Resveratrol Increases Lipolytic and Reduces Lipogenic Gene Expression under In Vitro Heat Stress Conditions in Dedifferentiated Adipocyte-Derived Progeny Cells from Dairy Cows |
| title_short | Antioxidant Resveratrol Increases Lipolytic and Reduces Lipogenic Gene Expression under In Vitro Heat Stress Conditions in Dedifferentiated Adipocyte-Derived Progeny Cells from Dairy Cows |
| title_sort | antioxidant resveratrol increases lipolytic and reduces lipogenic gene expression under in vitro heat stress conditions in dedifferentiated adipocyte derived progeny cells from dairy cows |
| topic | antioxidant DFAT cells docking <i>FASN</i> heat stress resveratrol |
| url | https://www.mdpi.com/2076-3921/10/6/905 |
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