Detection and Molecular Characterization of Rotavirus Infections in Children and Adults with Gastroenteritis from Vojvodina, Serbia
Rotaviruses (RV) are the leading cause of gastroenteritis in infants, young children, and adults, responsible for serious disease burden. In the period 2012–2018, a cross-sectional study was conducted using stool samples collected from patients with acute gastroenteritis from Vojvodina, Serbia. We d...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/10/2050 |
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| author | Aleksandra Patić Vladimir Vuković Gordana Kovačević Vladimir Petrović Mioljub Ristić Milan Djilas Petar Knežević Tatjana Pustahija Mirjana Štrbac Jelena Djekić Malbaša Smiljana Rajčević Ivana Hrnjaković Cvjetković |
| author_facet | Aleksandra Patić Vladimir Vuković Gordana Kovačević Vladimir Petrović Mioljub Ristić Milan Djilas Petar Knežević Tatjana Pustahija Mirjana Štrbac Jelena Djekić Malbaša Smiljana Rajčević Ivana Hrnjaković Cvjetković |
| author_sort | Aleksandra Patić |
| collection | DOAJ |
| description | Rotaviruses (RV) are the leading cause of gastroenteritis in infants, young children, and adults, responsible for serious disease burden. In the period 2012–2018, a cross-sectional study was conducted using stool samples collected from patients with acute gastroenteritis from Vojvodina, Serbia. We described age and gender distribution, as well as seasonal patterns of RV prevalence. Out of 1853 included stool samples, RV was detected in 29%. Hospitalized children between 1–2 years old were especially affected by RV infection (45%). The highest prevalence of infection was observed during the colder, winter/spring months. We compared sequenced representative G and P genotypes circulating in Serbia with vaccine strains and determined their genetic similarity. Genotype combination G2P[4] was the most prevalent (34.6%), followed by G2P[8] (24.1%) and G1P[8] (21.1%). Given that several epitopes were conserved, neutralization motifs among circulating strains can be characterized as sufficiently matching vaccine strains Rotarix™ and RotaTeq™, but existing antigenic disparities should not be overlooked. The present results contribute to a better insight into the prevalence of rotavirus infection in our region and point out the need for epidemiological surveillance of rotaviruses before the introduction of vaccines. These data can help formulate future vaccine strategies in Serbia. |
| first_indexed | 2024-03-09T19:44:22Z |
| format | Article |
| id | doaj.art-5d87e632316c46deaff060c2ce35fe1c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T19:44:22Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5d87e632316c46deaff060c2ce35fe1c2023-11-24T01:27:36ZengMDPI AGMicroorganisms2076-26072022-10-011010205010.3390/microorganisms10102050Detection and Molecular Characterization of Rotavirus Infections in Children and Adults with Gastroenteritis from Vojvodina, SerbiaAleksandra Patić0Vladimir Vuković1Gordana Kovačević2Vladimir Petrović3Mioljub Ristić4Milan Djilas5Petar Knežević6Tatjana Pustahija7Mirjana Štrbac8Jelena Djekić Malbaša9Smiljana Rajčević10Ivana Hrnjaković Cvjetković11Department of Microbiology with Parasitology and Immunology, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, SerbiaInstitute of Public Health of Vojvodina, 21000 Novi Sad, SerbiaInstitute of Public Health of Vojvodina, 21000 Novi Sad, SerbiaInstitute of Public Health of Vojvodina, 21000 Novi Sad, SerbiaInstitute of Public Health of Vojvodina, 21000 Novi Sad, SerbiaInstitute of Public Health of Vojvodina, 21000 Novi Sad, SerbiaFaculty of Sciences, University of Novi Sad, 21000 Novi Sad, SerbiaInstitute of Public Health of Vojvodina, 21000 Novi Sad, SerbiaInstitute of Public Health of Vojvodina, 21000 Novi Sad, SerbiaDepartment of Epidemiology, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, SerbiaInstitute of Public Health of Vojvodina, 21000 Novi Sad, SerbiaDepartment of Microbiology with Parasitology and Immunology, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, SerbiaRotaviruses (RV) are the leading cause of gastroenteritis in infants, young children, and adults, responsible for serious disease burden. In the period 2012–2018, a cross-sectional study was conducted using stool samples collected from patients with acute gastroenteritis from Vojvodina, Serbia. We described age and gender distribution, as well as seasonal patterns of RV prevalence. Out of 1853 included stool samples, RV was detected in 29%. Hospitalized children between 1–2 years old were especially affected by RV infection (45%). The highest prevalence of infection was observed during the colder, winter/spring months. We compared sequenced representative G and P genotypes circulating in Serbia with vaccine strains and determined their genetic similarity. Genotype combination G2P[4] was the most prevalent (34.6%), followed by G2P[8] (24.1%) and G1P[8] (21.1%). Given that several epitopes were conserved, neutralization motifs among circulating strains can be characterized as sufficiently matching vaccine strains Rotarix™ and RotaTeq™, but existing antigenic disparities should not be overlooked. The present results contribute to a better insight into the prevalence of rotavirus infection in our region and point out the need for epidemiological surveillance of rotaviruses before the introduction of vaccines. These data can help formulate future vaccine strategies in Serbia.https://www.mdpi.com/2076-2607/10/10/2050rotavirusgastroenteritisgenotypingantigenic epitopesvaccineSerbia |
| spellingShingle | Aleksandra Patić Vladimir Vuković Gordana Kovačević Vladimir Petrović Mioljub Ristić Milan Djilas Petar Knežević Tatjana Pustahija Mirjana Štrbac Jelena Djekić Malbaša Smiljana Rajčević Ivana Hrnjaković Cvjetković Detection and Molecular Characterization of Rotavirus Infections in Children and Adults with Gastroenteritis from Vojvodina, Serbia Microorganisms rotavirus gastroenteritis genotyping antigenic epitopes vaccine Serbia |
| title | Detection and Molecular Characterization of Rotavirus Infections in Children and Adults with Gastroenteritis from Vojvodina, Serbia |
| title_full | Detection and Molecular Characterization of Rotavirus Infections in Children and Adults with Gastroenteritis from Vojvodina, Serbia |
| title_fullStr | Detection and Molecular Characterization of Rotavirus Infections in Children and Adults with Gastroenteritis from Vojvodina, Serbia |
| title_full_unstemmed | Detection and Molecular Characterization of Rotavirus Infections in Children and Adults with Gastroenteritis from Vojvodina, Serbia |
| title_short | Detection and Molecular Characterization of Rotavirus Infections in Children and Adults with Gastroenteritis from Vojvodina, Serbia |
| title_sort | detection and molecular characterization of rotavirus infections in children and adults with gastroenteritis from vojvodina serbia |
| topic | rotavirus gastroenteritis genotyping antigenic epitopes vaccine Serbia |
| url | https://www.mdpi.com/2076-2607/10/10/2050 |
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