A quantitative 3D motility analysis of Trypanosoma brucei by use of digital in-line holographic microscopy.
We present a quantitative 3D analysis of the motility of the blood parasite Trypanosoma brucei. Digital in-line holographic microscopy has been used to track single cells with high temporal and spatial accuracy to obtain quantitative data on their behavior. Comparing bloodstream form and insect form...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3358310?pdf=render |
| _version_ | 1828183433916973056 |
|---|---|
| author | Sebastian Weiße Niko Heddergott Matthias Heydt Daniel Pflästerer Timo Maier Tamás Haraszti Michael Grunze Markus Engstler Axel Rosenhahn |
| author_facet | Sebastian Weiße Niko Heddergott Matthias Heydt Daniel Pflästerer Timo Maier Tamás Haraszti Michael Grunze Markus Engstler Axel Rosenhahn |
| author_sort | Sebastian Weiße |
| collection | DOAJ |
| description | We present a quantitative 3D analysis of the motility of the blood parasite Trypanosoma brucei. Digital in-line holographic microscopy has been used to track single cells with high temporal and spatial accuracy to obtain quantitative data on their behavior. Comparing bloodstream form and insect form trypanosomes as well as mutant and wildtype cells under varying external conditions we were able to derive a general two-state-run-and-tumble-model for trypanosome motility. Differences in the motility of distinct strains indicate that adaption of the trypanosomes to their natural environments involves a change in their mode of swimming. |
| first_indexed | 2024-04-12T06:33:42Z |
| format | Article |
| id | doaj.art-5d88281993284a0fae2db0db91f8451b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T06:33:42Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5d88281993284a0fae2db0db91f8451b2022-12-22T03:43:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3729610.1371/journal.pone.0037296A quantitative 3D motility analysis of Trypanosoma brucei by use of digital in-line holographic microscopy.Sebastian WeißeNiko HeddergottMatthias HeydtDaniel PflästererTimo MaierTamás HarasztiMichael GrunzeMarkus EngstlerAxel RosenhahnWe present a quantitative 3D analysis of the motility of the blood parasite Trypanosoma brucei. Digital in-line holographic microscopy has been used to track single cells with high temporal and spatial accuracy to obtain quantitative data on their behavior. Comparing bloodstream form and insect form trypanosomes as well as mutant and wildtype cells under varying external conditions we were able to derive a general two-state-run-and-tumble-model for trypanosome motility. Differences in the motility of distinct strains indicate that adaption of the trypanosomes to their natural environments involves a change in their mode of swimming.http://europepmc.org/articles/PMC3358310?pdf=render |
| spellingShingle | Sebastian Weiße Niko Heddergott Matthias Heydt Daniel Pflästerer Timo Maier Tamás Haraszti Michael Grunze Markus Engstler Axel Rosenhahn A quantitative 3D motility analysis of Trypanosoma brucei by use of digital in-line holographic microscopy. PLoS ONE |
| title | A quantitative 3D motility analysis of Trypanosoma brucei by use of digital in-line holographic microscopy. |
| title_full | A quantitative 3D motility analysis of Trypanosoma brucei by use of digital in-line holographic microscopy. |
| title_fullStr | A quantitative 3D motility analysis of Trypanosoma brucei by use of digital in-line holographic microscopy. |
| title_full_unstemmed | A quantitative 3D motility analysis of Trypanosoma brucei by use of digital in-line holographic microscopy. |
| title_short | A quantitative 3D motility analysis of Trypanosoma brucei by use of digital in-line holographic microscopy. |
| title_sort | quantitative 3d motility analysis of trypanosoma brucei by use of digital in line holographic microscopy |
| url | http://europepmc.org/articles/PMC3358310?pdf=render |
| work_keys_str_mv | AT sebastianweiße aquantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT nikoheddergott aquantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT matthiasheydt aquantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT danielpflasterer aquantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT timomaier aquantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT tamasharaszti aquantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT michaelgrunze aquantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT markusengstler aquantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT axelrosenhahn aquantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT sebastianweiße quantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT nikoheddergott quantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT matthiasheydt quantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT danielpflasterer quantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT timomaier quantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT tamasharaszti quantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT michaelgrunze quantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT markusengstler quantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy AT axelrosenhahn quantitative3dmotilityanalysisoftrypanosomabruceibyuseofdigitalinlineholographicmicroscopy |