Ubiquitination-related biomarkers in metastatic melanoma patients and their roles in tumor microenvironment
BackgroundSkin cutaneous melanoma (SKCM) is the deadliest type of cutaneous malignancy. Ubiquitination is a process of protein sorting and degradation that exhibits multiple functions in the progression of various tumors. This study aimed to characterize a set of genes for ubiquitination in SKCM.Met...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1170190/full |
| _version_ | 1797823700796440576 |
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| author | Li Zhang Zhehao Shi Fan Zhang Bin Chen Wei Qiu Lei Cai Xiaohua Lin |
| author_facet | Li Zhang Zhehao Shi Fan Zhang Bin Chen Wei Qiu Lei Cai Xiaohua Lin |
| author_sort | Li Zhang |
| collection | DOAJ |
| description | BackgroundSkin cutaneous melanoma (SKCM) is the deadliest type of cutaneous malignancy. Ubiquitination is a process of protein sorting and degradation that exhibits multiple functions in the progression of various tumors. This study aimed to characterize a set of genes for ubiquitination in SKCM.MethodsThe expression patterns of ubiquitin-associated genes (URGs) and the corresponding clinical information in SKCM tissues were comprehensively analyzed based on The Cancer Genome Atlas (TCGA) database. We performed univariate and multivariate Cox proportional regression models to characterize the risk scores and identify four critical genes related to prognostic ubiquitination (HCLS1, CORO1A, NCF1 and CCRL2), which were used to construct the prognostic signatures. We also studied the effects of HCLS1, CORO1A and CCRL2 on tumor metastasis-related indicators at the cellular level through in vitro experiments.ResultsSKCM patients in the low-risk group showing a longer survival than those in the high-risk group. Characteristic risk scores correlated with several clinicopathological variables and reflected the infiltration of multiple immune cells. In addition, the knockdown of CLS1, CORO1A and CCRL2 affected cellular malignant biological behavior through the EMT signaling pathway.ConclusionThis study provides a novel and prospective strategy to improve the clinical survival of SKCM patients. |
| first_indexed | 2024-03-13T10:27:52Z |
| format | Article |
| id | doaj.art-5d8fe1d8a9894ded94efc37646df969e |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-03-13T10:27:52Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-5d8fe1d8a9894ded94efc37646df969e2023-05-19T04:50:06ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-05-011310.3389/fonc.2023.11701901170190Ubiquitination-related biomarkers in metastatic melanoma patients and their roles in tumor microenvironmentLi ZhangZhehao ShiFan ZhangBin ChenWei QiuLei CaiXiaohua LinBackgroundSkin cutaneous melanoma (SKCM) is the deadliest type of cutaneous malignancy. Ubiquitination is a process of protein sorting and degradation that exhibits multiple functions in the progression of various tumors. This study aimed to characterize a set of genes for ubiquitination in SKCM.MethodsThe expression patterns of ubiquitin-associated genes (URGs) and the corresponding clinical information in SKCM tissues were comprehensively analyzed based on The Cancer Genome Atlas (TCGA) database. We performed univariate and multivariate Cox proportional regression models to characterize the risk scores and identify four critical genes related to prognostic ubiquitination (HCLS1, CORO1A, NCF1 and CCRL2), which were used to construct the prognostic signatures. We also studied the effects of HCLS1, CORO1A and CCRL2 on tumor metastasis-related indicators at the cellular level through in vitro experiments.ResultsSKCM patients in the low-risk group showing a longer survival than those in the high-risk group. Characteristic risk scores correlated with several clinicopathological variables and reflected the infiltration of multiple immune cells. In addition, the knockdown of CLS1, CORO1A and CCRL2 affected cellular malignant biological behavior through the EMT signaling pathway.ConclusionThis study provides a novel and prospective strategy to improve the clinical survival of SKCM patients.https://www.frontiersin.org/articles/10.3389/fonc.2023.1170190/fullskin cutaneous melanomaimmuneubiquitinationprognostic signature4-URGs |
| spellingShingle | Li Zhang Zhehao Shi Fan Zhang Bin Chen Wei Qiu Lei Cai Xiaohua Lin Ubiquitination-related biomarkers in metastatic melanoma patients and their roles in tumor microenvironment Frontiers in Oncology skin cutaneous melanoma immune ubiquitination prognostic signature 4-URGs |
| title | Ubiquitination-related biomarkers in metastatic melanoma patients and their roles in tumor microenvironment |
| title_full | Ubiquitination-related biomarkers in metastatic melanoma patients and their roles in tumor microenvironment |
| title_fullStr | Ubiquitination-related biomarkers in metastatic melanoma patients and their roles in tumor microenvironment |
| title_full_unstemmed | Ubiquitination-related biomarkers in metastatic melanoma patients and their roles in tumor microenvironment |
| title_short | Ubiquitination-related biomarkers in metastatic melanoma patients and their roles in tumor microenvironment |
| title_sort | ubiquitination related biomarkers in metastatic melanoma patients and their roles in tumor microenvironment |
| topic | skin cutaneous melanoma immune ubiquitination prognostic signature 4-URGs |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1170190/full |
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