Association of IL-1β +3954 G>A and IL-6 -174 G/C polymorphisms in congenital toxoplasmosis
Toxoplasmosis is caused by infection with the protozoan parasite Toxoplasma gondii, that has the capacity to infect all warm-blooded animals worldwide. The purpose of this investigation was to determine the distribution of genotypes and alleles in miscarriages woman as a result of Toxoplasma gondii...
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Finlay Ediciones
2021-09-01
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| Series: | VacciMonitor |
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| Online Access: | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2021000300125&lng=es&nrm=iso&tlng=en |
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| author | Nuha M. Mousa Hameed M. Jasim |
| author_facet | Nuha M. Mousa Hameed M. Jasim |
| author_sort | Nuha M. Mousa |
| collection | DOAJ |
| description | Toxoplasmosis is caused by infection with the protozoan parasite Toxoplasma gondii, that has the capacity to infect all warm-blooded animals worldwide. The purpose of this investigation was to determine the distribution of genotypes and alleles in miscarriages woman as a result of Toxoplasma gondii infection associated with interleukin-1β and interleukin-6 polymorphisms. A total of 125 miscarriage women suspected of toxoplasma infection and 50 healthy pregnant without previous miscarriage as control were enrolled in this study. The cases were screened for anti-toxoplasma IgM and IgG by ELISA test. Among the 125 miscarriage women, only 50 were positive to anti-Toxoplasma gondii IgG and IgM antibodies. The present study focused on assay the genotypes at IL-6 -174 G/C and IL-1β +3954 G>A to establish the associations between genetic polymorphisms and infection with Toxoplasma gondii. Results showed that the altered IL-1β GA, AA genotypes were high significant elevated in miscarriage women with toxoplasmosis (P=0.03), OR = 10 and 95% confidence intervals (1.32-81.48); (P=0.0007), OR = 0.07 and 95% confidence interval (0.01-0.32). The genotype GC at IL-6 (G/C) appears to be highly correlated with infection (P=0.01); OR = 3.18 and 95% confidence interval, (1.22- 8.30). In terms of allelic heterogeneity, C alleles were significantly more common in infected than uninfected cases for IL-6, while A allele is common in IL-1β single nucleotide polymorphisms (P =0.050). Furthermore, this study demonstrates that there is a strong and highly significant association between two forms of single nucleotide polymorphisms and the increased risk for toxoplasmosis. Genotypes of these polymorphism should be considered when evaluating genetic effects on toxoplasmosis incidence. However, to improve the prediction of this disease predisposition, a further study based on a larger cohort of patients is warranted. |
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| institution | Directory Open Access Journal |
| issn | 1025-028X 1025-0298 |
| language | English |
| last_indexed | 2024-12-24T03:53:57Z |
| publishDate | 2021-09-01 |
| publisher | Finlay Ediciones |
| record_format | Article |
| series | VacciMonitor |
| spelling | doaj.art-5d91028a867c468eabc32af678b4abe02022-12-21T17:16:30ZengFinlay EdicionesVacciMonitor1025-028X1025-02982021-09-01303125132Association of IL-1β +3954 G>A and IL-6 -174 G/C polymorphisms in congenital toxoplasmosisNuha M. Mousa0https://orcid.org/0000-0003-1564-0361Hameed M. Jasim1https://orcid.org/0000-0001-5966-0554Al-Muthana University, College of Science, IraqAl-Nahrain University, College of Biotechnology, IraqToxoplasmosis is caused by infection with the protozoan parasite Toxoplasma gondii, that has the capacity to infect all warm-blooded animals worldwide. The purpose of this investigation was to determine the distribution of genotypes and alleles in miscarriages woman as a result of Toxoplasma gondii infection associated with interleukin-1β and interleukin-6 polymorphisms. A total of 125 miscarriage women suspected of toxoplasma infection and 50 healthy pregnant without previous miscarriage as control were enrolled in this study. The cases were screened for anti-toxoplasma IgM and IgG by ELISA test. Among the 125 miscarriage women, only 50 were positive to anti-Toxoplasma gondii IgG and IgM antibodies. The present study focused on assay the genotypes at IL-6 -174 G/C and IL-1β +3954 G>A to establish the associations between genetic polymorphisms and infection with Toxoplasma gondii. Results showed that the altered IL-1β GA, AA genotypes were high significant elevated in miscarriage women with toxoplasmosis (P=0.03), OR = 10 and 95% confidence intervals (1.32-81.48); (P=0.0007), OR = 0.07 and 95% confidence interval (0.01-0.32). The genotype GC at IL-6 (G/C) appears to be highly correlated with infection (P=0.01); OR = 3.18 and 95% confidence interval, (1.22- 8.30). In terms of allelic heterogeneity, C alleles were significantly more common in infected than uninfected cases for IL-6, while A allele is common in IL-1β single nucleotide polymorphisms (P =0.050). Furthermore, this study demonstrates that there is a strong and highly significant association between two forms of single nucleotide polymorphisms and the increased risk for toxoplasmosis. Genotypes of these polymorphism should be considered when evaluating genetic effects on toxoplasmosis incidence. However, to improve the prediction of this disease predisposition, a further study based on a larger cohort of patients is warranted.http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2021000300125&lng=es&nrm=iso&tlng=entoxoplasma gondiisingle nucleotide polymorphismsgenotype |
| spellingShingle | Nuha M. Mousa Hameed M. Jasim Association of IL-1β +3954 G>A and IL-6 -174 G/C polymorphisms in congenital toxoplasmosis VacciMonitor toxoplasma gondii single nucleotide polymorphisms genotype |
| title | Association of IL-1β +3954 G>A and IL-6 -174 G/C polymorphisms in congenital toxoplasmosis |
| title_full | Association of IL-1β +3954 G>A and IL-6 -174 G/C polymorphisms in congenital toxoplasmosis |
| title_fullStr | Association of IL-1β +3954 G>A and IL-6 -174 G/C polymorphisms in congenital toxoplasmosis |
| title_full_unstemmed | Association of IL-1β +3954 G>A and IL-6 -174 G/C polymorphisms in congenital toxoplasmosis |
| title_short | Association of IL-1β +3954 G>A and IL-6 -174 G/C polymorphisms in congenital toxoplasmosis |
| title_sort | association of il 1β 3954 g a and il 6 174 g c polymorphisms in congenital toxoplasmosis |
| topic | toxoplasma gondii single nucleotide polymorphisms genotype |
| url | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2021000300125&lng=es&nrm=iso&tlng=en |
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