Heritable stochastic switching revealed by single-cell genealogy.
The partitioning and subsequent inheritance of cellular factors like proteins and RNAs is a ubiquitous feature of cell division. However, direct quantitative measures of how such nongenetic inheritance affects subsequent changes in gene expression have been lacking. We tracked families of the yeast...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2007-09-01
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| Series: | PLoS Biology |
| Online Access: | http://europepmc.org/articles/PMC1964776?pdf=render |
| _version_ | 1819011371848695808 |
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| author | Benjamin B Kaufmann Qiong Yang Jerome T Mettetal Alexander van Oudenaarden |
| author_facet | Benjamin B Kaufmann Qiong Yang Jerome T Mettetal Alexander van Oudenaarden |
| author_sort | Benjamin B Kaufmann |
| collection | DOAJ |
| description | The partitioning and subsequent inheritance of cellular factors like proteins and RNAs is a ubiquitous feature of cell division. However, direct quantitative measures of how such nongenetic inheritance affects subsequent changes in gene expression have been lacking. We tracked families of the yeast Saccharomyces cerevisiae as they switch between two semi-stable epigenetic states. We found that long after two cells have divided, they continued to switch in a synchronized manner, whereas individual cells have exponentially distributed switching times. By comparing these results to a Poisson process, we show that the time evolution of an epigenetic state depends initially on inherited factors, with stochastic processes requiring several generations to decorrelate closely related cells. Finally, a simple stochastic model demonstrates that a single fluctuating regulatory protein that is synthesized in large bursts can explain the bulk of our results. |
| first_indexed | 2024-12-21T01:27:06Z |
| format | Article |
| id | doaj.art-5d932dd988794b608467033bd7081bfd |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-21T01:27:06Z |
| publishDate | 2007-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-5d932dd988794b608467033bd7081bfd2022-12-21T19:20:28ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852007-09-0159e23910.1371/journal.pbio.0050239Heritable stochastic switching revealed by single-cell genealogy.Benjamin B KaufmannQiong YangJerome T MettetalAlexander van OudenaardenThe partitioning and subsequent inheritance of cellular factors like proteins and RNAs is a ubiquitous feature of cell division. However, direct quantitative measures of how such nongenetic inheritance affects subsequent changes in gene expression have been lacking. We tracked families of the yeast Saccharomyces cerevisiae as they switch between two semi-stable epigenetic states. We found that long after two cells have divided, they continued to switch in a synchronized manner, whereas individual cells have exponentially distributed switching times. By comparing these results to a Poisson process, we show that the time evolution of an epigenetic state depends initially on inherited factors, with stochastic processes requiring several generations to decorrelate closely related cells. Finally, a simple stochastic model demonstrates that a single fluctuating regulatory protein that is synthesized in large bursts can explain the bulk of our results.http://europepmc.org/articles/PMC1964776?pdf=render |
| spellingShingle | Benjamin B Kaufmann Qiong Yang Jerome T Mettetal Alexander van Oudenaarden Heritable stochastic switching revealed by single-cell genealogy. PLoS Biology |
| title | Heritable stochastic switching revealed by single-cell genealogy. |
| title_full | Heritable stochastic switching revealed by single-cell genealogy. |
| title_fullStr | Heritable stochastic switching revealed by single-cell genealogy. |
| title_full_unstemmed | Heritable stochastic switching revealed by single-cell genealogy. |
| title_short | Heritable stochastic switching revealed by single-cell genealogy. |
| title_sort | heritable stochastic switching revealed by single cell genealogy |
| url | http://europepmc.org/articles/PMC1964776?pdf=render |
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