Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis
Chronic periodontitis (CP) is an oral cavity disease arising from chronic inflammation of the periodontal tissues. Exosomes are lipid vesicles that are enriched in specific microRNAs (miRNAs), potentially providing a disease-specific diagnostic signature. To assess the value of exosomal miRNAs as bi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2020.587381/full |
| _version_ | 1818648838654656512 |
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| author | Nik Nur Syazana Nik Mohamed Kamal Raja Azman Raja Awang Suharni Mohamad Wan Nazatul Shima Shahidan |
| author_facet | Nik Nur Syazana Nik Mohamed Kamal Raja Azman Raja Awang Suharni Mohamad Wan Nazatul Shima Shahidan |
| author_sort | Nik Nur Syazana Nik Mohamed Kamal |
| collection | DOAJ |
| description | Chronic periodontitis (CP) is an oral cavity disease arising from chronic inflammation of the periodontal tissues. Exosomes are lipid vesicles that are enriched in specific microRNAs (miRNAs), potentially providing a disease-specific diagnostic signature. To assess the value of exosomal miRNAs as biomarkers for CP, 8 plasma- and 8 salivary-exosomal miRNAs samples were profiled using Agilent platform (comparative study). From 2,549 probed miRNAs, 33 miRNAs were significantly down-regulated in CP as compared to healthy plasma samples. Whereas, 1,995 miRNAs (1,985 down-regulated and 10 up-regulated) were differentially expressed in the CP as compared to healthy saliva samples. hsa-miR-let-7d [FC = −26.76; AUC = 1; r = −0.728 [p-value = 0.04]), hsa-miR-126-3p (FC = −24.02; AUC = 1; r = −0.723 [p-value = 0.043]) and hsa-miR-199a-3p (FC = −22.94; AUC = 1; r = −0.731 [p-value = 0.039]) are worth to be furthered studied for plasma-exosomal samples. Meanwhile, for salivary-exosomal samples, hsa-miR-125a-3p (FC = 2.03; AUC = 1; r = 0.91 [p-value = 0.02]) is worth to be furthered studied. These miRNAs are the reliable candidates for the development of periodontitis biomarker, as they were significantly expressed differently between CP and healthy samples, have a good discriminatory value and strongly correlate with the mean of PPD. These findings highlight the potential of exosomal miRNAs profiling in the diagnosis from both sourced as well as provide new insights into the molecular mechanisms involved in CP. |
| first_indexed | 2024-12-17T01:24:47Z |
| format | Article |
| id | doaj.art-5d953f2e50a54e329276b8311c574e51 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-17T01:24:47Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-5d953f2e50a54e329276b8311c574e512022-12-21T22:08:42ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-11-011110.3389/fphys.2020.587381587381Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic PeriodontitisNik Nur Syazana Nik Mohamed KamalRaja Azman Raja AwangSuharni MohamadWan Nazatul Shima ShahidanChronic periodontitis (CP) is an oral cavity disease arising from chronic inflammation of the periodontal tissues. Exosomes are lipid vesicles that are enriched in specific microRNAs (miRNAs), potentially providing a disease-specific diagnostic signature. To assess the value of exosomal miRNAs as biomarkers for CP, 8 plasma- and 8 salivary-exosomal miRNAs samples were profiled using Agilent platform (comparative study). From 2,549 probed miRNAs, 33 miRNAs were significantly down-regulated in CP as compared to healthy plasma samples. Whereas, 1,995 miRNAs (1,985 down-regulated and 10 up-regulated) were differentially expressed in the CP as compared to healthy saliva samples. hsa-miR-let-7d [FC = −26.76; AUC = 1; r = −0.728 [p-value = 0.04]), hsa-miR-126-3p (FC = −24.02; AUC = 1; r = −0.723 [p-value = 0.043]) and hsa-miR-199a-3p (FC = −22.94; AUC = 1; r = −0.731 [p-value = 0.039]) are worth to be furthered studied for plasma-exosomal samples. Meanwhile, for salivary-exosomal samples, hsa-miR-125a-3p (FC = 2.03; AUC = 1; r = 0.91 [p-value = 0.02]) is worth to be furthered studied. These miRNAs are the reliable candidates for the development of periodontitis biomarker, as they were significantly expressed differently between CP and healthy samples, have a good discriminatory value and strongly correlate with the mean of PPD. These findings highlight the potential of exosomal miRNAs profiling in the diagnosis from both sourced as well as provide new insights into the molecular mechanisms involved in CP.https://www.frontiersin.org/articles/10.3389/fphys.2020.587381/fullbiomarkerinflammationprofilingmiRNA – microRNAexosome |
| spellingShingle | Nik Nur Syazana Nik Mohamed Kamal Raja Azman Raja Awang Suharni Mohamad Wan Nazatul Shima Shahidan Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis Frontiers in Physiology biomarker inflammation profiling miRNA – microRNA exosome |
| title | Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis |
| title_full | Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis |
| title_fullStr | Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis |
| title_full_unstemmed | Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis |
| title_short | Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis |
| title_sort | plasma and saliva exosome profile reveals a distinct microrna signature in chronic periodontitis |
| topic | biomarker inflammation profiling miRNA – microRNA exosome |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2020.587381/full |
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