Ectopic Expression of a Wheat R2R3-Type MYB Gene in Transgenic Tobacco Enhances Osmotic Stress Tolerance via Maintaining ROS Balance and Improving Root System Architecture
Water scarcity is a critical cause of plant yield loss and decreased quality. Manipulation of root system architecture to minimize the impact of water scarcity stresses may greatly contribute towards an improved distribution of roots in the soil and enhanced water and nutrient uptake abilities. In t...
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MDPI AG
2024-02-01
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| author | Omar Azab Walid Ben Romdhane Salah El-Hendawy Abdelhalim Ghazy Adel M. Zakri Ahmed M. Abd-ElGawad Abdullah Al-Doss |
| author_facet | Omar Azab Walid Ben Romdhane Salah El-Hendawy Abdelhalim Ghazy Adel M. Zakri Ahmed M. Abd-ElGawad Abdullah Al-Doss |
| author_sort | Omar Azab |
| collection | DOAJ |
| description | Water scarcity is a critical cause of plant yield loss and decreased quality. Manipulation of root system architecture to minimize the impact of water scarcity stresses may greatly contribute towards an improved distribution of roots in the soil and enhanced water and nutrient uptake abilities. In this study, we explored the potential of <i>TaMYB20</i> gene, a wheat gene belonging to the R2R3-MYB transcription factor family, to improve root system architecture in transgenic tobacco plants. The full-length <i>TaMYB20</i> gene was isolated from <i>Triticum aestivum</i>.cv. Sakha94 and used to produce genetically engineered tobacco plants. The transgenic plants exhibited enhanced tolerance to extended osmotic stress and were able to maintain their root system architecture traits, including total root length (TRL), lateral root number (LRN), root surface area (RSa), and root volume (RV), while the wild-type plants failed to maintain the same traits. The transgenic lines presented greater relative water content in their roots associated with decreased ion leakage. The oxidative stress resulted in the loss of mitochondrial membrane integrity in the wild-type (WT) plants due to the overproduction of reactive oxygen species (ROS) in the root cells, while the transgenic lines were able to scavenge the excess ROS under stressful conditions through the activation of the redox system. Finally, we found that the steady-state levels of three <i>PIN</i> gene transcripts were greater in the <i>TaMYB20</i>-transgenic lines compared to the wild-type tobacco. Taken together, these findings confirm that <i>TaMYB20</i> is a potentially useful gene candidate for engineering drought tolerance in cultivated plants. |
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| language | English |
| last_indexed | 2024-03-07T22:41:38Z |
| publishDate | 2024-02-01 |
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| series | Biology |
| spelling | doaj.art-5d9cf337ddd44c369a62d5c00f6129892024-02-23T15:08:15ZengMDPI AGBiology2079-77372024-02-0113212810.3390/biology13020128Ectopic Expression of a Wheat R2R3-Type MYB Gene in Transgenic Tobacco Enhances Osmotic Stress Tolerance via Maintaining ROS Balance and Improving Root System ArchitectureOmar Azab0Walid Ben Romdhane1Salah El-Hendawy2Abdelhalim Ghazy3Adel M. Zakri4Ahmed M. Abd-ElGawad5Abdullah Al-Doss6Plant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaWater scarcity is a critical cause of plant yield loss and decreased quality. Manipulation of root system architecture to minimize the impact of water scarcity stresses may greatly contribute towards an improved distribution of roots in the soil and enhanced water and nutrient uptake abilities. In this study, we explored the potential of <i>TaMYB20</i> gene, a wheat gene belonging to the R2R3-MYB transcription factor family, to improve root system architecture in transgenic tobacco plants. The full-length <i>TaMYB20</i> gene was isolated from <i>Triticum aestivum</i>.cv. Sakha94 and used to produce genetically engineered tobacco plants. The transgenic plants exhibited enhanced tolerance to extended osmotic stress and were able to maintain their root system architecture traits, including total root length (TRL), lateral root number (LRN), root surface area (RSa), and root volume (RV), while the wild-type plants failed to maintain the same traits. The transgenic lines presented greater relative water content in their roots associated with decreased ion leakage. The oxidative stress resulted in the loss of mitochondrial membrane integrity in the wild-type (WT) plants due to the overproduction of reactive oxygen species (ROS) in the root cells, while the transgenic lines were able to scavenge the excess ROS under stressful conditions through the activation of the redox system. Finally, we found that the steady-state levels of three <i>PIN</i> gene transcripts were greater in the <i>TaMYB20</i>-transgenic lines compared to the wild-type tobacco. Taken together, these findings confirm that <i>TaMYB20</i> is a potentially useful gene candidate for engineering drought tolerance in cultivated plants.https://www.mdpi.com/2079-7737/13/2/128osmotic stressroot system architectureR2R3-type MYBwheat |
| spellingShingle | Omar Azab Walid Ben Romdhane Salah El-Hendawy Abdelhalim Ghazy Adel M. Zakri Ahmed M. Abd-ElGawad Abdullah Al-Doss Ectopic Expression of a Wheat R2R3-Type MYB Gene in Transgenic Tobacco Enhances Osmotic Stress Tolerance via Maintaining ROS Balance and Improving Root System Architecture Biology osmotic stress root system architecture R2R3-type MYB wheat |
| title | Ectopic Expression of a Wheat R2R3-Type MYB Gene in Transgenic Tobacco Enhances Osmotic Stress Tolerance via Maintaining ROS Balance and Improving Root System Architecture |
| title_full | Ectopic Expression of a Wheat R2R3-Type MYB Gene in Transgenic Tobacco Enhances Osmotic Stress Tolerance via Maintaining ROS Balance and Improving Root System Architecture |
| title_fullStr | Ectopic Expression of a Wheat R2R3-Type MYB Gene in Transgenic Tobacco Enhances Osmotic Stress Tolerance via Maintaining ROS Balance and Improving Root System Architecture |
| title_full_unstemmed | Ectopic Expression of a Wheat R2R3-Type MYB Gene in Transgenic Tobacco Enhances Osmotic Stress Tolerance via Maintaining ROS Balance and Improving Root System Architecture |
| title_short | Ectopic Expression of a Wheat R2R3-Type MYB Gene in Transgenic Tobacco Enhances Osmotic Stress Tolerance via Maintaining ROS Balance and Improving Root System Architecture |
| title_sort | ectopic expression of a wheat r2r3 type myb gene in transgenic tobacco enhances osmotic stress tolerance via maintaining ros balance and improving root system architecture |
| topic | osmotic stress root system architecture R2R3-type MYB wheat |
| url | https://www.mdpi.com/2079-7737/13/2/128 |
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