Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk
Staphylococcus aureus is one of the most important pathogenic bacteria in the dairy products industry that is capable of biofilm formation. Biofilm formation by these bacteria has been led to resistance to antimicrobial agents. Inactivation of microorganisms using cold atmospheric plasma is one of t...
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Islamic Azad University, Tabriz Branch
2020-03-01
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Series: | Bihdāsht-i Mavādd-i Ghaz̠āyī |
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Online Access: | http://jfh.iaut.ac.ir/article_671045_8d0269e102fc08047abadb5a1183c8c2.pdf |
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author | F. Jahandideh J. Shayegh S. Hosseinzadeh |
author_facet | F. Jahandideh J. Shayegh S. Hosseinzadeh |
author_sort | F. Jahandideh |
collection | DOAJ |
description | Staphylococcus aureus is one of the most important pathogenic bacteria in the dairy products industry that is capable of biofilm formation. Biofilm formation by these bacteria has been led to resistance to antimicrobial agents. Inactivation of microorganisms using cold atmospheric plasma is one of the new approaches in the food industry. In this study, to evaluate the antibacterial and anti-biofilm effect of cold atmospheric plasma, a dielectric barrier discharge system was used. Twenty isolates of S. aureus from clinical bovine mastitis milk were exposed to plasma from 5 to 20 Sec and their antibacterial activity was estimated by recording the growth zone of inhibition. Plasma treatment was performed punctually for 5 Sec to assess the possible effects of plasma treatment on bacterial biofilm-formation activity after 24 h. The results show that a remarkable reduction in the growth of bacteria by increasing the flow of plasma. Moreover, statistical analysis of the ELISA reader results showed that the exposure of the isolates to plasma, significantly (p < 0.05) reduced the biofilm formation. These results suggest that plasma can be a suitable alternative method for thermal sterilization techniques. However, its application requires further studies to determine the severity and duration of exposure of microorganisms to plasma. |
first_indexed | 2024-12-17T20:27:48Z |
format | Article |
id | doaj.art-5d9d95698ee94c38a8894bd7080c1de8 |
institution | Directory Open Access Journal |
issn | 2228-7647 2476-6968 |
language | fas |
last_indexed | 2024-12-17T20:27:48Z |
publishDate | 2020-03-01 |
publisher | Islamic Azad University, Tabriz Branch |
record_format | Article |
series | Bihdāsht-i Mavādd-i Ghaz̠āyī |
spelling | doaj.art-5d9d95698ee94c38a8894bd7080c1de82022-12-21T21:33:41ZfasIslamic Azad University, Tabriz BranchBihdāsht-i Mavādd-i Ghaz̠āyī2228-76472476-69682020-03-01101 (37) بهار9110210.30495/jfh.2020.671045671045Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milkF. Jahandideh0J. Shayegh1S. Hosseinzadeh2Graduated in Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, IranAssistant Professor, Department of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, IranAssistant Professor, Department of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, IranStaphylococcus aureus is one of the most important pathogenic bacteria in the dairy products industry that is capable of biofilm formation. Biofilm formation by these bacteria has been led to resistance to antimicrobial agents. Inactivation of microorganisms using cold atmospheric plasma is one of the new approaches in the food industry. In this study, to evaluate the antibacterial and anti-biofilm effect of cold atmospheric plasma, a dielectric barrier discharge system was used. Twenty isolates of S. aureus from clinical bovine mastitis milk were exposed to plasma from 5 to 20 Sec and their antibacterial activity was estimated by recording the growth zone of inhibition. Plasma treatment was performed punctually for 5 Sec to assess the possible effects of plasma treatment on bacterial biofilm-formation activity after 24 h. The results show that a remarkable reduction in the growth of bacteria by increasing the flow of plasma. Moreover, statistical analysis of the ELISA reader results showed that the exposure of the isolates to plasma, significantly (p < 0.05) reduced the biofilm formation. These results suggest that plasma can be a suitable alternative method for thermal sterilization techniques. However, its application requires further studies to determine the severity and duration of exposure of microorganisms to plasma.http://jfh.iaut.ac.ir/article_671045_8d0269e102fc08047abadb5a1183c8c2.pdfcold atmospheric plasmadielectric barrier dischargestaphylococcus aureusbiofilmmilk |
spellingShingle | F. Jahandideh J. Shayegh S. Hosseinzadeh Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk Bihdāsht-i Mavādd-i Ghaz̠āyī cold atmospheric plasma dielectric barrier discharge staphylococcus aureus biofilm milk |
title | Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk |
title_full | Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk |
title_fullStr | Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk |
title_full_unstemmed | Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk |
title_short | Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk |
title_sort | effect of cold atmospheric plasma on growth and biofilm formation of staphylococcus aureus isolated from mastitic bovine milk |
topic | cold atmospheric plasma dielectric barrier discharge staphylococcus aureus biofilm milk |
url | http://jfh.iaut.ac.ir/article_671045_8d0269e102fc08047abadb5a1183c8c2.pdf |
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