Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk

Staphylococcus aureus is one of the most important pathogenic bacteria in the dairy products industry that is capable of biofilm formation. Biofilm formation by these bacteria has been led to resistance to antimicrobial agents. Inactivation of microorganisms using cold atmospheric plasma is one of t...

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Main Authors: F. Jahandideh, J. Shayegh, S. Hosseinzadeh
Format: Article
Language:fas
Published: Islamic Azad University, Tabriz Branch 2020-03-01
Series:Bihdāsht-i Mavādd-i Ghaz̠āyī
Subjects:
Online Access:http://jfh.iaut.ac.ir/article_671045_8d0269e102fc08047abadb5a1183c8c2.pdf
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author F. Jahandideh
J. Shayegh
S. Hosseinzadeh
author_facet F. Jahandideh
J. Shayegh
S. Hosseinzadeh
author_sort F. Jahandideh
collection DOAJ
description Staphylococcus aureus is one of the most important pathogenic bacteria in the dairy products industry that is capable of biofilm formation. Biofilm formation by these bacteria has been led to resistance to antimicrobial agents. Inactivation of microorganisms using cold atmospheric plasma is one of the new approaches in the food industry. In this study, to evaluate the antibacterial and anti-biofilm effect of cold atmospheric plasma, a dielectric barrier discharge system was used. Twenty isolates of S. aureus from clinical bovine mastitis milk were exposed to plasma from 5 to 20 Sec and their antibacterial activity was estimated by recording the growth zone of inhibition. Plasma treatment was performed punctually for 5 Sec to assess the possible effects of plasma treatment on bacterial biofilm-formation activity after 24 h. The results show that a remarkable reduction in the growth of bacteria by increasing the flow of plasma. Moreover, statistical analysis of the ELISA reader results showed that the exposure of the isolates to plasma, significantly (p < 0.05) reduced the biofilm formation. These results suggest that plasma can be a suitable alternative method for thermal sterilization techniques. However, its application requires further studies to determine the severity and duration of exposure of microorganisms to plasma.
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spelling doaj.art-5d9d95698ee94c38a8894bd7080c1de82022-12-21T21:33:41ZfasIslamic Azad University, Tabriz BranchBihdāsht-i Mavādd-i Ghaz̠āyī2228-76472476-69682020-03-01101 (37) بهار9110210.30495/jfh.2020.671045671045Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milkF. Jahandideh0J. Shayegh1S. Hosseinzadeh2Graduated in Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, IranAssistant Professor, Department of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, IranAssistant Professor, Department of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, IranStaphylococcus aureus is one of the most important pathogenic bacteria in the dairy products industry that is capable of biofilm formation. Biofilm formation by these bacteria has been led to resistance to antimicrobial agents. Inactivation of microorganisms using cold atmospheric plasma is one of the new approaches in the food industry. In this study, to evaluate the antibacterial and anti-biofilm effect of cold atmospheric plasma, a dielectric barrier discharge system was used. Twenty isolates of S. aureus from clinical bovine mastitis milk were exposed to plasma from 5 to 20 Sec and their antibacterial activity was estimated by recording the growth zone of inhibition. Plasma treatment was performed punctually for 5 Sec to assess the possible effects of plasma treatment on bacterial biofilm-formation activity after 24 h. The results show that a remarkable reduction in the growth of bacteria by increasing the flow of plasma. Moreover, statistical analysis of the ELISA reader results showed that the exposure of the isolates to plasma, significantly (p < 0.05) reduced the biofilm formation. These results suggest that plasma can be a suitable alternative method for thermal sterilization techniques. However, its application requires further studies to determine the severity and duration of exposure of microorganisms to plasma.http://jfh.iaut.ac.ir/article_671045_8d0269e102fc08047abadb5a1183c8c2.pdfcold atmospheric plasmadielectric barrier dischargestaphylococcus aureusbiofilmmilk
spellingShingle F. Jahandideh
J. Shayegh
S. Hosseinzadeh
Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk
Bihdāsht-i Mavādd-i Ghaz̠āyī
cold atmospheric plasma
dielectric barrier discharge
staphylococcus aureus
biofilm
milk
title Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk
title_full Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk
title_fullStr Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk
title_full_unstemmed Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk
title_short Effect of cold atmospheric plasma on growth and biofilm formation of Staphylococcus aureus isolated from mastitic bovine milk
title_sort effect of cold atmospheric plasma on growth and biofilm formation of staphylococcus aureus isolated from mastitic bovine milk
topic cold atmospheric plasma
dielectric barrier discharge
staphylococcus aureus
biofilm
milk
url http://jfh.iaut.ac.ir/article_671045_8d0269e102fc08047abadb5a1183c8c2.pdf
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AT shosseinzadeh effectofcoldatmosphericplasmaongrowthandbiofilmformationofstaphylococcusaureusisolatedfrommastiticbovinemilk