Knockdown Resistance (kdr) Mutations I1532T and F1534S Were Identified in Aedes albopictus Field Populations in Zhejiang Province, Central China
Aedes albopictus is the only vector that can transmit the dengue virus in Zhejiang Province, central China, and it can develop insecticide resistance due to long-term exposure to pyrethroids. The presence of knockdown resistance (kdr) mutations is one of the mechanisms responsible for pyrethroid res...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-06-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.702081/full |
| _version_ | 1818739465672196096 |
|---|---|
| author | Yuyan Wu Qinmei Liu Yunpeng Qi Yinping Wu Qinxiang Ni Weihua Chen Jinna Wang Tianqi Li Mingyu Luo Juan Hou Zhenyu Gong Jimin Sun |
| author_facet | Yuyan Wu Qinmei Liu Yunpeng Qi Yinping Wu Qinxiang Ni Weihua Chen Jinna Wang Tianqi Li Mingyu Luo Juan Hou Zhenyu Gong Jimin Sun |
| author_sort | Yuyan Wu |
| collection | DOAJ |
| description | Aedes albopictus is the only vector that can transmit the dengue virus in Zhejiang Province, central China, and it can develop insecticide resistance due to long-term exposure to pyrethroids. The presence of knockdown resistance (kdr) mutations is one of the mechanisms responsible for pyrethroid resistance, and has been reported in some Ae. albopictus populations in southern China. However, little is known about the DNA diversity of the voltage-gated sodium channel (VGSC) gene in Ae. albopictus populations in central China. Four Ae. albopictus field populations were collected, in Yiwu (YW), Quzhou (QZ), Wenzhou (WZ), and Jiaxing (JX) from Zhejiang Province, central China. The susceptibility of Ae. albopictus adults to three pyrethroids (beta-cypermethrin, deltamethrin, and permethrin) was tested using the WHO tube assay, and Kdr mutations were identified via PCR and sequencing. The relationship between kdr mutations and pyrethroid phenotypes was also analyzed. Of the four populations, none was sensitive to any pyrethroid tested, and the YW population showed the strongest pyrethroid resistance. Non-synonymous kdr mutations were detected in codons 1532 and 1534, domain III. At codon 1534, one mutant allele, TCC(S), was detected in the four populations with a frequency of 42.08%, while at codon 1532, one mutant allele, ACC(T), was detected in the JX and QZ populations, with frequencies of 4.22 and 3.03%, respectively. The F1534S mutant allele was positively correlated with both beta-cypermethrin and deltamethrin resistance phenotypes (OR > 1, P < 0.05), whereas the I1532T mutant allele was possibly negatively correlated with beta-cypermethrin, deltamethrin, and permethrin resistance phenotypes (OR < 1, P > 0.05). In conclusion, resistance and resistance mutations regarding to three pyrethroids are already present in the Ae. Albopictus populations from Zhejiang, central China, which prompts the need to use non-insecticide-based methods of insect control. |
| first_indexed | 2024-12-18T01:25:16Z |
| format | Article |
| id | doaj.art-5d9f4f9e54f749cbb76be24e674f1456 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-18T01:25:16Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-5d9f4f9e54f749cbb76be24e674f14562022-12-21T21:25:45ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-06-011110.3389/fcimb.2021.702081702081Knockdown Resistance (kdr) Mutations I1532T and F1534S Were Identified in Aedes albopictus Field Populations in Zhejiang Province, Central ChinaYuyan Wu0Qinmei Liu1Yunpeng Qi2Yinping Wu3Qinxiang Ni4Weihua Chen5Jinna Wang6Tianqi Li7Mingyu Luo8Juan Hou9Zhenyu Gong10Jimin Sun11Department of Infectious Diseases Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou City, ChinaDepartment of Infectious Diseases Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou City, ChinaDepartment of Infectious Diseases Control and Prevention, Jiaxing Center for Disease Control and Prevention, Jiaxing City, ChinaDepartment of Vector Control and Prevention, Yiwu Center for Disease Control and Prevention, Yiwu City, ChinaDepartment of Infectious Diseases Control and Prevention, Wenzhou Center for Disease Control and Prevention, Wenzhou City, ChinaDepartment of Infectious Diseases Control and Prevention, Quzhou Center for Disease Control and Prevention, Quzhou City, ChinaDepartment of Infectious Diseases Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou City, ChinaDepartment of Infectious Diseases Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou City, ChinaDepartment of Infectious Diseases Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou City, ChinaDepartment of Infectious Diseases Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou City, ChinaDepartment of Infectious Diseases Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou City, ChinaDepartment of Infectious Diseases Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou City, ChinaAedes albopictus is the only vector that can transmit the dengue virus in Zhejiang Province, central China, and it can develop insecticide resistance due to long-term exposure to pyrethroids. The presence of knockdown resistance (kdr) mutations is one of the mechanisms responsible for pyrethroid resistance, and has been reported in some Ae. albopictus populations in southern China. However, little is known about the DNA diversity of the voltage-gated sodium channel (VGSC) gene in Ae. albopictus populations in central China. Four Ae. albopictus field populations were collected, in Yiwu (YW), Quzhou (QZ), Wenzhou (WZ), and Jiaxing (JX) from Zhejiang Province, central China. The susceptibility of Ae. albopictus adults to three pyrethroids (beta-cypermethrin, deltamethrin, and permethrin) was tested using the WHO tube assay, and Kdr mutations were identified via PCR and sequencing. The relationship between kdr mutations and pyrethroid phenotypes was also analyzed. Of the four populations, none was sensitive to any pyrethroid tested, and the YW population showed the strongest pyrethroid resistance. Non-synonymous kdr mutations were detected in codons 1532 and 1534, domain III. At codon 1534, one mutant allele, TCC(S), was detected in the four populations with a frequency of 42.08%, while at codon 1532, one mutant allele, ACC(T), was detected in the JX and QZ populations, with frequencies of 4.22 and 3.03%, respectively. The F1534S mutant allele was positively correlated with both beta-cypermethrin and deltamethrin resistance phenotypes (OR > 1, P < 0.05), whereas the I1532T mutant allele was possibly negatively correlated with beta-cypermethrin, deltamethrin, and permethrin resistance phenotypes (OR < 1, P > 0.05). In conclusion, resistance and resistance mutations regarding to three pyrethroids are already present in the Ae. Albopictus populations from Zhejiang, central China, which prompts the need to use non-insecticide-based methods of insect control.https://www.frontiersin.org/articles/10.3389/fcimb.2021.702081/fullAedes albopictuspyrethroidinsecticide resistancekdrCentral China |
| spellingShingle | Yuyan Wu Qinmei Liu Yunpeng Qi Yinping Wu Qinxiang Ni Weihua Chen Jinna Wang Tianqi Li Mingyu Luo Juan Hou Zhenyu Gong Jimin Sun Knockdown Resistance (kdr) Mutations I1532T and F1534S Were Identified in Aedes albopictus Field Populations in Zhejiang Province, Central China Frontiers in Cellular and Infection Microbiology Aedes albopictus pyrethroid insecticide resistance kdr Central China |
| title | Knockdown Resistance (kdr) Mutations I1532T and F1534S Were Identified in Aedes albopictus Field Populations in Zhejiang Province, Central China |
| title_full | Knockdown Resistance (kdr) Mutations I1532T and F1534S Were Identified in Aedes albopictus Field Populations in Zhejiang Province, Central China |
| title_fullStr | Knockdown Resistance (kdr) Mutations I1532T and F1534S Were Identified in Aedes albopictus Field Populations in Zhejiang Province, Central China |
| title_full_unstemmed | Knockdown Resistance (kdr) Mutations I1532T and F1534S Were Identified in Aedes albopictus Field Populations in Zhejiang Province, Central China |
| title_short | Knockdown Resistance (kdr) Mutations I1532T and F1534S Were Identified in Aedes albopictus Field Populations in Zhejiang Province, Central China |
| title_sort | knockdown resistance kdr mutations i1532t and f1534s were identified in aedes albopictus field populations in zhejiang province central china |
| topic | Aedes albopictus pyrethroid insecticide resistance kdr Central China |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.702081/full |
| work_keys_str_mv | AT yuyanwu knockdownresistancekdrmutationsi1532tandf1534swereidentifiedinaedesalbopictusfieldpopulationsinzhejiangprovincecentralchina AT qinmeiliu knockdownresistancekdrmutationsi1532tandf1534swereidentifiedinaedesalbopictusfieldpopulationsinzhejiangprovincecentralchina AT yunpengqi knockdownresistancekdrmutationsi1532tandf1534swereidentifiedinaedesalbopictusfieldpopulationsinzhejiangprovincecentralchina AT yinpingwu knockdownresistancekdrmutationsi1532tandf1534swereidentifiedinaedesalbopictusfieldpopulationsinzhejiangprovincecentralchina AT qinxiangni knockdownresistancekdrmutationsi1532tandf1534swereidentifiedinaedesalbopictusfieldpopulationsinzhejiangprovincecentralchina AT weihuachen knockdownresistancekdrmutationsi1532tandf1534swereidentifiedinaedesalbopictusfieldpopulationsinzhejiangprovincecentralchina AT jinnawang knockdownresistancekdrmutationsi1532tandf1534swereidentifiedinaedesalbopictusfieldpopulationsinzhejiangprovincecentralchina AT tianqili knockdownresistancekdrmutationsi1532tandf1534swereidentifiedinaedesalbopictusfieldpopulationsinzhejiangprovincecentralchina AT mingyuluo knockdownresistancekdrmutationsi1532tandf1534swereidentifiedinaedesalbopictusfieldpopulationsinzhejiangprovincecentralchina AT juanhou knockdownresistancekdrmutationsi1532tandf1534swereidentifiedinaedesalbopictusfieldpopulationsinzhejiangprovincecentralchina AT zhenyugong knockdownresistancekdrmutationsi1532tandf1534swereidentifiedinaedesalbopictusfieldpopulationsinzhejiangprovincecentralchina AT jiminsun knockdownresistancekdrmutationsi1532tandf1534swereidentifiedinaedesalbopictusfieldpopulationsinzhejiangprovincecentralchina |