Antioxidant and antiproliferative activities of n-hexane extract and its fractions from Blumea balsamifera L. leaves
Blumea balsamifera is a plant species that has been popularly used to treat a broad spectrum of diseases. In efforts of tackling the increasing threat of cancers, B. balsamifera has been studied for its anticancer potentials. Hence, through this research, we aimed to evaluate the antioxidant and ant...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
| Subjects: | |
| Online Access: | http://www.japtr.org/article.asp?issn=2231-4040;year=2022;volume=13;issue=3;spage=216;epage=220;aulast=Ginting |
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| author | Binawati Ginting Ilham Maulana Mustanir Yahya Nurdin Saidi Murniana Murniana Kartini Hasballah Maulidna Maulidna Siti Rawati |
| author_facet | Binawati Ginting Ilham Maulana Mustanir Yahya Nurdin Saidi Murniana Murniana Kartini Hasballah Maulidna Maulidna Siti Rawati |
| author_sort | Binawati Ginting |
| collection | DOAJ |
| description | Blumea balsamifera is a plant species that has been popularly used to treat a broad spectrum of diseases. In efforts of tackling the increasing threat of cancers, B. balsamifera has been studied for its anticancer potentials. Hence, through this research, we aimed to evaluate the antioxidant and antiproliferative activities of n-hexane extract from B. balsamifera L. leaves along with its fractionation products. After the n-hexane extract has been obtained, the sample was column chromatographed using gradient elution with n-hexane:ethyl acetate solvent. All the isolation protocols produced 1 n-hexane extract and 10 different fractions (fractions 1–10). Antioxidant and antiproliferative effects of the samples were assessed based on 2,2-diphenyl-1-picrylhydrazyl and brine shrimp lethality test assay, respectively. None of the samples have a strong antioxidant level because all samples yielded IC50 of more than 100 ppm – the best of them was fraction 8 with IC50 = 113.716 ppm. On contrary, most of the samples were observed to have a potent antiproliferative effect, especially fraction 8 with LC50 = 2.00 ppm. Taken altogether, fraction 8 from the n-hexane extract of B. balsamifera L. leaves is the most potential candidate for proliferative disease therapy, where further studies confirming the results are required. |
| first_indexed | 2024-04-13T05:40:15Z |
| format | Article |
| id | doaj.art-5da3d1eefaeb4cc8850fd34cda75ee48 |
| institution | Directory Open Access Journal |
| issn | 2231-4040 0976-2094 |
| language | English |
| last_indexed | 2024-04-13T05:40:15Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Advanced Pharmaceutical Technology & Research |
| spelling | doaj.art-5da3d1eefaeb4cc8850fd34cda75ee482022-12-22T03:00:08ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research2231-40400976-20942022-01-0113321622010.4103/japtr.japtr_105_22Antioxidant and antiproliferative activities of n-hexane extract and its fractions from Blumea balsamifera L. leavesBinawati GintingIlham MaulanaMustanir YahyaNurdin SaidiMurniana MurnianaKartini HasballahMaulidna MaulidnaSiti RawatiBlumea balsamifera is a plant species that has been popularly used to treat a broad spectrum of diseases. In efforts of tackling the increasing threat of cancers, B. balsamifera has been studied for its anticancer potentials. Hence, through this research, we aimed to evaluate the antioxidant and antiproliferative activities of n-hexane extract from B. balsamifera L. leaves along with its fractionation products. After the n-hexane extract has been obtained, the sample was column chromatographed using gradient elution with n-hexane:ethyl acetate solvent. All the isolation protocols produced 1 n-hexane extract and 10 different fractions (fractions 1–10). Antioxidant and antiproliferative effects of the samples were assessed based on 2,2-diphenyl-1-picrylhydrazyl and brine shrimp lethality test assay, respectively. None of the samples have a strong antioxidant level because all samples yielded IC50 of more than 100 ppm – the best of them was fraction 8 with IC50 = 113.716 ppm. On contrary, most of the samples were observed to have a potent antiproliferative effect, especially fraction 8 with LC50 = 2.00 ppm. Taken altogether, fraction 8 from the n-hexane extract of B. balsamifera L. leaves is the most potential candidate for proliferative disease therapy, where further studies confirming the results are required.http://www.japtr.org/article.asp?issn=2231-4040;year=2022;volume=13;issue=3;spage=216;epage=220;aulast=Gintingantioxidantantiproliferativebrine shrimp lethality test22-diphenyl-1-picrylhydrazylphytomedicine |
| spellingShingle | Binawati Ginting Ilham Maulana Mustanir Yahya Nurdin Saidi Murniana Murniana Kartini Hasballah Maulidna Maulidna Siti Rawati Antioxidant and antiproliferative activities of n-hexane extract and its fractions from Blumea balsamifera L. leaves Journal of Advanced Pharmaceutical Technology & Research antioxidant antiproliferative brine shrimp lethality test 2 2-diphenyl-1-picrylhydrazyl phytomedicine |
| title | Antioxidant and antiproliferative activities of n-hexane extract and its fractions from Blumea balsamifera L. leaves |
| title_full | Antioxidant and antiproliferative activities of n-hexane extract and its fractions from Blumea balsamifera L. leaves |
| title_fullStr | Antioxidant and antiproliferative activities of n-hexane extract and its fractions from Blumea balsamifera L. leaves |
| title_full_unstemmed | Antioxidant and antiproliferative activities of n-hexane extract and its fractions from Blumea balsamifera L. leaves |
| title_short | Antioxidant and antiproliferative activities of n-hexane extract and its fractions from Blumea balsamifera L. leaves |
| title_sort | antioxidant and antiproliferative activities of n hexane extract and its fractions from blumea balsamifera l leaves |
| topic | antioxidant antiproliferative brine shrimp lethality test 2 2-diphenyl-1-picrylhydrazyl phytomedicine |
| url | http://www.japtr.org/article.asp?issn=2231-4040;year=2022;volume=13;issue=3;spage=216;epage=220;aulast=Ginting |
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