Investigation of Basophil Activation Test for Diagnosing Milk and Egg Allergy in Younger Children
In children with concomitant food allergy and atopic dermatitis (AD), uncovering the causative food allergen is more arduous. We evaluated the basophil activation test (BAT) for its diagnostic value in children, including those with AD, for milk or egg allergy. We simultaneously measured serum-speci...
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MDPI AG
2020-12-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/12/3942 |
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| author | Yoon Hee Kim Young Suh Kim Younhee Park Soo Yeon Kim Kyung Won Kim Hyon Suk Kim Myung Hyun Sohn |
| author_facet | Yoon Hee Kim Young Suh Kim Younhee Park Soo Yeon Kim Kyung Won Kim Hyon Suk Kim Myung Hyun Sohn |
| author_sort | Yoon Hee Kim |
| collection | DOAJ |
| description | In children with concomitant food allergy and atopic dermatitis (AD), uncovering the causative food allergen is more arduous. We evaluated the basophil activation test (BAT) for its diagnostic value in children, including those with AD, for milk or egg allergy. We simultaneously measured serum-specific immunoglobulin E (sIgE) levels and performed BATs for cow’s milk and egg white. We compared their overall diagnostic performance using the area under the receiver operating characteristic curve (AUC) with the Delong method and compared them in children with AD. Analyses were completed for 75 children for milk allergy and for 85 children for egg allergy. The sIgE and percentage of basophils with the expression of CD63 were correlated for both milk (r = 0.384, <i>p</i> < 0.001) and egg (r = 0.557, <i>p</i> < 0.001). The AUC of sIgE (0.701) for milk allergy was significantly increased when combined with the BAT (0.805; <i>p</i> = 0.029). In children with AD, the AUC of the BAT (0.924) for milk allergy was significantly larger than that of sIgE (0.701; <i>p</i> = 0.017). The BAT is a potentially useful diagnostic tool for milk allergy in children when combined with sIgE. Moreover, it may be a surrogate marker for milk allergy in children with AD. |
| first_indexed | 2024-03-10T14:18:23Z |
| format | Article |
| id | doaj.art-5da5dc6aa3f841cfbf5afa81b10d9256 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T14:18:23Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-5da5dc6aa3f841cfbf5afa81b10d92562023-11-20T23:35:40ZengMDPI AGJournal of Clinical Medicine2077-03832020-12-01912394210.3390/jcm9123942Investigation of Basophil Activation Test for Diagnosing Milk and Egg Allergy in Younger ChildrenYoon Hee Kim0Young Suh Kim1Younhee Park2Soo Yeon Kim3Kyung Won Kim4Hyon Suk Kim5Myung Hyun Sohn6Department of Pediatrics, Institute of Allergy, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Pediatrics, Institute of Allergy, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Laboratory Medicine, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Pediatrics, Institute of Allergy, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Pediatrics, Institute of Allergy, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Laboratory Medicine, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Pediatrics, Institute of Allergy, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 03722, KoreaIn children with concomitant food allergy and atopic dermatitis (AD), uncovering the causative food allergen is more arduous. We evaluated the basophil activation test (BAT) for its diagnostic value in children, including those with AD, for milk or egg allergy. We simultaneously measured serum-specific immunoglobulin E (sIgE) levels and performed BATs for cow’s milk and egg white. We compared their overall diagnostic performance using the area under the receiver operating characteristic curve (AUC) with the Delong method and compared them in children with AD. Analyses were completed for 75 children for milk allergy and for 85 children for egg allergy. The sIgE and percentage of basophils with the expression of CD63 were correlated for both milk (r = 0.384, <i>p</i> < 0.001) and egg (r = 0.557, <i>p</i> < 0.001). The AUC of sIgE (0.701) for milk allergy was significantly increased when combined with the BAT (0.805; <i>p</i> = 0.029). In children with AD, the AUC of the BAT (0.924) for milk allergy was significantly larger than that of sIgE (0.701; <i>p</i> = 0.017). The BAT is a potentially useful diagnostic tool for milk allergy in children when combined with sIgE. Moreover, it may be a surrogate marker for milk allergy in children with AD.https://www.mdpi.com/2077-0383/9/12/3942atopic dermatitisanaphylaxisbasophil activation testchilddiagnosisfood allergy |
| spellingShingle | Yoon Hee Kim Young Suh Kim Younhee Park Soo Yeon Kim Kyung Won Kim Hyon Suk Kim Myung Hyun Sohn Investigation of Basophil Activation Test for Diagnosing Milk and Egg Allergy in Younger Children Journal of Clinical Medicine atopic dermatitis anaphylaxis basophil activation test child diagnosis food allergy |
| title | Investigation of Basophil Activation Test for Diagnosing Milk and Egg Allergy in Younger Children |
| title_full | Investigation of Basophil Activation Test for Diagnosing Milk and Egg Allergy in Younger Children |
| title_fullStr | Investigation of Basophil Activation Test for Diagnosing Milk and Egg Allergy in Younger Children |
| title_full_unstemmed | Investigation of Basophil Activation Test for Diagnosing Milk and Egg Allergy in Younger Children |
| title_short | Investigation of Basophil Activation Test for Diagnosing Milk and Egg Allergy in Younger Children |
| title_sort | investigation of basophil activation test for diagnosing milk and egg allergy in younger children |
| topic | atopic dermatitis anaphylaxis basophil activation test child diagnosis food allergy |
| url | https://www.mdpi.com/2077-0383/9/12/3942 |
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