Trichoderma asperellum in the biocontrol of Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense
Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense are pathogens causing trunk canker in Carya illioniensis and there are still no reports of effective forms of control. However, biological control is a promising measure. The objective of this work was to isolate, identify and evaluate the...
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2023-12-01
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| Series: | Journal of Plant Protection Research |
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| Online Access: | https://journals.pan.pl/Content/129617/PDF/OA_12_JPPR_63_4_1745_Rabuske.pdf |
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| author | Jéssica Emília Rabuske Marlove Fátima Brião Muniz Thiarles Brun Mateus Alves Saldanha Janaina Silva Sarzi Lucas Gracioli Savian Clair Walker Jéssica Mengue Rolim Giovani Leone Zabot Marcio Antonio Mazutti |
| author_facet | Jéssica Emília Rabuske Marlove Fátima Brião Muniz Thiarles Brun Mateus Alves Saldanha Janaina Silva Sarzi Lucas Gracioli Savian Clair Walker Jéssica Mengue Rolim Giovani Leone Zabot Marcio Antonio Mazutti |
| author_sort | Jéssica Emília Rabuske |
| collection | DOAJ |
| description | Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense are pathogens causing trunk canker in Carya illioniensis and there are still no reports of effective forms of control. However, biological control is a promising measure. The objective of this work was to isolate, identify and evaluate the action of Trichoderma spp. in the in vitro control of L. theobromae and P. kimberleyense and to identify the compounds produced by the antagonist. Trichoderma spp. was identified by molecular technique and morphologically characterized. The antagonistic action of T. asperellum isolates (obtained from the rhizospheric soil, and of an isolate obtained from a commercial formulation) was evaluated by pairing cultures and volatile metabolites on L. theobromae and P. kimberleyense. Optimization of the cultivation method of T. asperellum was carried out and the compounds produced by the antagonist were identified by gas chromatography. Isolates obtained from the soil were identified as T. asperellum and decreased mycelial growth of L. theobromae and P. kimberleyense in the crop pairing test (48.98% S6 x Qt), as well as by volatile metabolites (29.85% SM21 x TR4). The cultivation conditions that generated the filtrates with the greatest antifungal action used 20 g · l –1 of corn maceration water, yeast extract 7.5 g · l –1, pH 5, agitation 100 rpm, sucrose 50 g · l –1, inoculum concentration 105 spores /ml. Among the identified compounds, some stood out for having bioactive action, such as pyran derivatives, celidoniol, deoxy, pentadecanoicacid, 2,3-dihydro-3,5-dihydroxy-6-methyl, propanoicacid, 1-methylethyl ester and 9-octadecenoic acid. The T. asperellum isolates showed potential for biocontrol in vitro, acting by different mechanisms. |
| first_indexed | 2024-03-08T22:00:03Z |
| format | Article |
| id | doaj.art-5da74a70f447405983bfc0ac9c4cf010 |
| institution | Directory Open Access Journal |
| issn | 1427-4345 1899-007X |
| language | English |
| last_indexed | 2024-03-08T22:00:03Z |
| publishDate | 2023-12-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Plant Protection Research |
| spelling | doaj.art-5da74a70f447405983bfc0ac9c4cf0102023-12-19T15:32:43ZengPolish Academy of SciencesJournal of Plant Protection Research1427-43451899-007X2023-12-01vol. 63No 4488498https://doi.org/10.24425/jppr.2023.147832Trichoderma asperellum in the biocontrol of Lasiodiplodia theobromae and Pseudofusicoccum kimberleyenseJéssica Emília Rabuske0https://orcid.org/0000-0003-2938-1671Marlove Fátima Brião Muniz1https://orcid.org/0000-0001-7436-9589Thiarles Brun2https://orcid.org/0000-0002-3330-0347Mateus Alves Saldanha3https://orcid.org/0000-0002-1788-8179Janaina Silva Sarzi4https://orcid.org/0000-0002-2447-3115Lucas Gracioli Savian5https://orcid.org/0000-0002-4398-7998Clair Walker6https://orcid.org/0000-0002-6707-3963Jéssica Mengue Rolim7https://orcid.org/0000-0003-2737-7599Giovani Leone Zabot8https://orcid.org/0000-0001-7933-6161Marcio Antonio Mazutti9https://orcid.org/0000-0001-8217-5629Universidade Regional Integrada do Alto Uruguai e das Missões, Frederico Westphalen, Rio Grande do Sul, BrazilDepartamento de Defesa Fitossanitária, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, BrazilUniversidade Regional Integrada do Alto Uruguai e das Missões, Frederico Westphalen, Rio Grande do Sul, BrazilDepartamento de Defesa Fitossanitária, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, BrazilDepartamento de Defesa Fitossanitária, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, BrazilDepartamento de Defesa Fitossanitária, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, BrazilDepartamento de Defesa Fitossanitária, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, BrazilDepartamento de Fitotecnia, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, BrazilLaboratório de Processos de Engenharia Agroindustrial, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, BrazilDepartamento de Engenharia Química, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, BrazilLasiodiplodia theobromae and Pseudofusicoccum kimberleyense are pathogens causing trunk canker in Carya illioniensis and there are still no reports of effective forms of control. However, biological control is a promising measure. The objective of this work was to isolate, identify and evaluate the action of Trichoderma spp. in the in vitro control of L. theobromae and P. kimberleyense and to identify the compounds produced by the antagonist. Trichoderma spp. was identified by molecular technique and morphologically characterized. The antagonistic action of T. asperellum isolates (obtained from the rhizospheric soil, and of an isolate obtained from a commercial formulation) was evaluated by pairing cultures and volatile metabolites on L. theobromae and P. kimberleyense. Optimization of the cultivation method of T. asperellum was carried out and the compounds produced by the antagonist were identified by gas chromatography. Isolates obtained from the soil were identified as T. asperellum and decreased mycelial growth of L. theobromae and P. kimberleyense in the crop pairing test (48.98% S6 x Qt), as well as by volatile metabolites (29.85% SM21 x TR4). The cultivation conditions that generated the filtrates with the greatest antifungal action used 20 g · l –1 of corn maceration water, yeast extract 7.5 g · l –1, pH 5, agitation 100 rpm, sucrose 50 g · l –1, inoculum concentration 105 spores /ml. Among the identified compounds, some stood out for having bioactive action, such as pyran derivatives, celidoniol, deoxy, pentadecanoicacid, 2,3-dihydro-3,5-dihydroxy-6-methyl, propanoicacid, 1-methylethyl ester and 9-octadecenoic acid. The T. asperellum isolates showed potential for biocontrol in vitro, acting by different mechanisms.https://journals.pan.pl/Content/129617/PDF/OA_12_JPPR_63_4_1745_Rabuske.pdfcarya illinoinensischromatographic analysisculture filtratessecondary metabolites |
| spellingShingle | Jéssica Emília Rabuske Marlove Fátima Brião Muniz Thiarles Brun Mateus Alves Saldanha Janaina Silva Sarzi Lucas Gracioli Savian Clair Walker Jéssica Mengue Rolim Giovani Leone Zabot Marcio Antonio Mazutti Trichoderma asperellum in the biocontrol of Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense Journal of Plant Protection Research carya illinoinensis chromatographic analysis culture filtrates secondary metabolites |
| title | Trichoderma asperellum in the biocontrol of Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense |
| title_full | Trichoderma asperellum in the biocontrol of Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense |
| title_fullStr | Trichoderma asperellum in the biocontrol of Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense |
| title_full_unstemmed | Trichoderma asperellum in the biocontrol of Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense |
| title_short | Trichoderma asperellum in the biocontrol of Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense |
| title_sort | trichoderma asperellum in the biocontrol of lasiodiplodia theobromae and pseudofusicoccum kimberleyense |
| topic | carya illinoinensis chromatographic analysis culture filtrates secondary metabolites |
| url | https://journals.pan.pl/Content/129617/PDF/OA_12_JPPR_63_4_1745_Rabuske.pdf |
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