Natural exosomes-like nanoparticles in mung bean sprouts possesses anti-diabetic effects via activation of PI3K/Akt/GLUT4/GSK-3β signaling pathway
Abstract Background Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease characterized by hyperglycemia and insulin resistance. Mung bean sprouts are traditionally considered a “folk” hypoglycemic food and their pharmacological effects and underlying mechanisms warrant further investigatio...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-09-01
|
| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-023-02120-w |
| _version_ | 1797556717388562432 |
|---|---|
| author | Chengxun He Ke Wang Jun Xia Die Qian Juan Guo Lian Zhong Dandan Tang Xiuping Chen Wei Peng Yunhui Chen Yong Tang |
| author_facet | Chengxun He Ke Wang Jun Xia Die Qian Juan Guo Lian Zhong Dandan Tang Xiuping Chen Wei Peng Yunhui Chen Yong Tang |
| author_sort | Chengxun He |
| collection | DOAJ |
| description | Abstract Background Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease characterized by hyperglycemia and insulin resistance. Mung bean sprouts are traditionally considered a “folk” hypoglycemic food and their pharmacological effects and underlying mechanisms warrant further investigation. Purpose This study aimed to investigate the anti-diabetic effects of the exosomes-like nanoparticles in mung bean sprouts (MELNs) and explore the related molecular mechanisms. Results MELNs were isolated using a differential centrifugation-polyethylene glycol (PEG) method, and the identification of MELNs were confirmed by PAGE gel electrophoresis, agarose gel electrophoresis, thin-layer chromatography (TLC), and transmission electron microscopy (TEM). In the high-fat diet/streptozotocin (HFD/STZ) mouse model, MELNs ameliorated the progression of T2DM by increasing oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) results, decreasing the fasting blood glucose level, and reducing the serum triglycerides (TG) and total cholesterol (TC). Histopathological examinations indicated MELNs diminished inflammatory infiltration of hepatocytes and amplified the area of islet B cells. In addition, MELNs decreased the oxidative stress levels in liver tissue and had good biocompatibility. In vitro experiments verified that MELNs improved the viability of glucosamine (GlcN) induced insulin-resistant hepatocytes. Furthermore, this study also revealed that MELNs upregulated GLUT4 & Nrf2 and down-regulated GSK-3β via activating the PI3K/Akt signaling pathway, promoting the production of antioxidant enzymes, such as HO-1 and SOD, to reduce oxidative stress. Conclusion MELNs mitigated the progression of type 2 diabetes in HFD/STZ mouse model. The underlying molecular mechanism is related to PI3K/Akt/GLUT4/GSK-3β signaling pathway. |
| first_indexed | 2024-03-10T17:06:50Z |
| format | Article |
| id | doaj.art-5da7a223a1f64da79f35b11944ab00cf |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-03-10T17:06:50Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-5da7a223a1f64da79f35b11944ab00cf2023-11-20T10:47:32ZengBMCJournal of Nanobiotechnology1477-31552023-09-0121111710.1186/s12951-023-02120-wNatural exosomes-like nanoparticles in mung bean sprouts possesses anti-diabetic effects via activation of PI3K/Akt/GLUT4/GSK-3β signaling pathwayChengxun He0Ke Wang1Jun Xia2Die Qian3Juan Guo4Lian Zhong5Dandan Tang6Xiuping Chen7Wei Peng8Yunhui Chen9Yong Tang10State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of Traditional Chinese MedicineHospital of Chengdu University of Traditional Chinese MedicineState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of Traditional Chinese MedicineState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of Traditional Chinese MedicineState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of Traditional Chinese MedicineState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of Traditional Chinese MedicineState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of Traditional Chinese MedicineState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of Traditional Chinese MedicineState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of Traditional Chinese MedicineState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of Traditional Chinese MedicineAbstract Background Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease characterized by hyperglycemia and insulin resistance. Mung bean sprouts are traditionally considered a “folk” hypoglycemic food and their pharmacological effects and underlying mechanisms warrant further investigation. Purpose This study aimed to investigate the anti-diabetic effects of the exosomes-like nanoparticles in mung bean sprouts (MELNs) and explore the related molecular mechanisms. Results MELNs were isolated using a differential centrifugation-polyethylene glycol (PEG) method, and the identification of MELNs were confirmed by PAGE gel electrophoresis, agarose gel electrophoresis, thin-layer chromatography (TLC), and transmission electron microscopy (TEM). In the high-fat diet/streptozotocin (HFD/STZ) mouse model, MELNs ameliorated the progression of T2DM by increasing oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) results, decreasing the fasting blood glucose level, and reducing the serum triglycerides (TG) and total cholesterol (TC). Histopathological examinations indicated MELNs diminished inflammatory infiltration of hepatocytes and amplified the area of islet B cells. In addition, MELNs decreased the oxidative stress levels in liver tissue and had good biocompatibility. In vitro experiments verified that MELNs improved the viability of glucosamine (GlcN) induced insulin-resistant hepatocytes. Furthermore, this study also revealed that MELNs upregulated GLUT4 & Nrf2 and down-regulated GSK-3β via activating the PI3K/Akt signaling pathway, promoting the production of antioxidant enzymes, such as HO-1 and SOD, to reduce oxidative stress. Conclusion MELNs mitigated the progression of type 2 diabetes in HFD/STZ mouse model. The underlying molecular mechanism is related to PI3K/Akt/GLUT4/GSK-3β signaling pathway.https://doi.org/10.1186/s12951-023-02120-wType 2 diabetes MellitusMung bean sproutsExosome-like nanoparticlesOxidative stress |
| spellingShingle | Chengxun He Ke Wang Jun Xia Die Qian Juan Guo Lian Zhong Dandan Tang Xiuping Chen Wei Peng Yunhui Chen Yong Tang Natural exosomes-like nanoparticles in mung bean sprouts possesses anti-diabetic effects via activation of PI3K/Akt/GLUT4/GSK-3β signaling pathway Journal of Nanobiotechnology Type 2 diabetes Mellitus Mung bean sprouts Exosome-like nanoparticles Oxidative stress |
| title | Natural exosomes-like nanoparticles in mung bean sprouts possesses anti-diabetic effects via activation of PI3K/Akt/GLUT4/GSK-3β signaling pathway |
| title_full | Natural exosomes-like nanoparticles in mung bean sprouts possesses anti-diabetic effects via activation of PI3K/Akt/GLUT4/GSK-3β signaling pathway |
| title_fullStr | Natural exosomes-like nanoparticles in mung bean sprouts possesses anti-diabetic effects via activation of PI3K/Akt/GLUT4/GSK-3β signaling pathway |
| title_full_unstemmed | Natural exosomes-like nanoparticles in mung bean sprouts possesses anti-diabetic effects via activation of PI3K/Akt/GLUT4/GSK-3β signaling pathway |
| title_short | Natural exosomes-like nanoparticles in mung bean sprouts possesses anti-diabetic effects via activation of PI3K/Akt/GLUT4/GSK-3β signaling pathway |
| title_sort | natural exosomes like nanoparticles in mung bean sprouts possesses anti diabetic effects via activation of pi3k akt glut4 gsk 3β signaling pathway |
| topic | Type 2 diabetes Mellitus Mung bean sprouts Exosome-like nanoparticles Oxidative stress |
| url | https://doi.org/10.1186/s12951-023-02120-w |
| work_keys_str_mv | AT chengxunhe naturalexosomeslikenanoparticlesinmungbeansproutspossessesantidiabeticeffectsviaactivationofpi3kaktglut4gsk3bsignalingpathway AT kewang naturalexosomeslikenanoparticlesinmungbeansproutspossessesantidiabeticeffectsviaactivationofpi3kaktglut4gsk3bsignalingpathway AT junxia naturalexosomeslikenanoparticlesinmungbeansproutspossessesantidiabeticeffectsviaactivationofpi3kaktglut4gsk3bsignalingpathway AT dieqian naturalexosomeslikenanoparticlesinmungbeansproutspossessesantidiabeticeffectsviaactivationofpi3kaktglut4gsk3bsignalingpathway AT juanguo naturalexosomeslikenanoparticlesinmungbeansproutspossessesantidiabeticeffectsviaactivationofpi3kaktglut4gsk3bsignalingpathway AT lianzhong naturalexosomeslikenanoparticlesinmungbeansproutspossessesantidiabeticeffectsviaactivationofpi3kaktglut4gsk3bsignalingpathway AT dandantang naturalexosomeslikenanoparticlesinmungbeansproutspossessesantidiabeticeffectsviaactivationofpi3kaktglut4gsk3bsignalingpathway AT xiupingchen naturalexosomeslikenanoparticlesinmungbeansproutspossessesantidiabeticeffectsviaactivationofpi3kaktglut4gsk3bsignalingpathway AT weipeng naturalexosomeslikenanoparticlesinmungbeansproutspossessesantidiabeticeffectsviaactivationofpi3kaktglut4gsk3bsignalingpathway AT yunhuichen naturalexosomeslikenanoparticlesinmungbeansproutspossessesantidiabeticeffectsviaactivationofpi3kaktglut4gsk3bsignalingpathway AT yongtang naturalexosomeslikenanoparticlesinmungbeansproutspossessesantidiabeticeffectsviaactivationofpi3kaktglut4gsk3bsignalingpathway |