Seed Viability Test, Germination Rate and In vitro Regeneration of Some Alkanna Species
In this study, it was aimed to test the seed viability of Alkanna orientalis and endemic Alkanna sieheana species rich in Alkannin/Shikonin (A/S) and to determine the germination percentage and callus regeneration suitable for in vitro A/S production. The viability rates of seeds collected from na...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Siirt University
2021-06-01
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| Series: | Türkiye Tarımsal Araştırmalar Dergisi |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/pub/tutad/issue/62689/892928 |
| _version_ | 1797907083941642240 |
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| author | Cennet YAMAN Serkan URANBEY Hussein Abdullah Ahmed AHMED Dilek BAŞALMA |
| author_facet | Cennet YAMAN Serkan URANBEY Hussein Abdullah Ahmed AHMED Dilek BAŞALMA |
| author_sort | Cennet YAMAN |
| collection | DOAJ |
| description | In this study, it was aimed to test the seed viability of Alkanna orientalis and endemic Alkanna sieheana species
rich in Alkannin/Shikonin (A/S) and to determine the germination percentage and callus regeneration suitable for in vitro
A/S production. The viability rates of seeds collected from nature were determined by the tetrazolium method. The
germination rates of the seeds of both species were tested with three different applications (on filter paper, MS nutrient
medium with agar, in peat). The seed coats of both species were removed, and their embryos were cultivated in Murashige
and Skoog (MS) media containing plant growth regulators in various proportions. The viability rates of the seeds were found
at 98.3% for A. orientalis and 96.0% for A. sieheana. Germination rates were determined only on filter paper as 8.3% for A.
orientalis and 5.0% for A. sieheana, no germination was observed in other applications. The highest zygotic shoot and root
formation rate were obtained from A. orientalis with 91.6% and 40.3%, respectively. But, the highest callus formation rate
was observed on A. siehenea with 75.0%. As a result, it was determined that although the viability rates were high in both
species, the germination rates were low, but in vitro reproducibility was possible. |
| first_indexed | 2024-04-10T10:30:51Z |
| format | Article |
| id | doaj.art-5da7da60de6d415ca85f97b71fcc9639 |
| institution | Directory Open Access Journal |
| issn | 2148-2306 2528-858X |
| language | English |
| last_indexed | 2024-04-10T10:30:51Z |
| publishDate | 2021-06-01 |
| publisher | Siirt University |
| record_format | Article |
| series | Türkiye Tarımsal Araştırmalar Dergisi |
| spelling | doaj.art-5da7da60de6d415ca85f97b71fcc96392023-02-15T16:21:08ZengSiirt UniversityTürkiye Tarımsal Araştırmalar Dergisi2148-23062528-858X2021-06-018222022710.19159/tutad.892928Seed Viability Test, Germination Rate and In vitro Regeneration of Some Alkanna SpeciesCennet YAMAN0https://orcid.org/0000-0002-2364-8171 Serkan URANBEY1https://orcid.org/0000-0002-0312-8099Hussein Abdullah Ahmed AHMED2https://orcid.org/0000-0003-2634-6854Dilek BAŞALMA3https://orcid.org/0000-0003-4748-5546Yozgat Bozok Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Yozgat, TÜRKİYEAnkara Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Ankara, TÜRKİYEAnkara Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Ankara, TÜRKİYEAnkara Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Ankara, TÜRKİYEIn this study, it was aimed to test the seed viability of Alkanna orientalis and endemic Alkanna sieheana species rich in Alkannin/Shikonin (A/S) and to determine the germination percentage and callus regeneration suitable for in vitro A/S production. The viability rates of seeds collected from nature were determined by the tetrazolium method. The germination rates of the seeds of both species were tested with three different applications (on filter paper, MS nutrient medium with agar, in peat). The seed coats of both species were removed, and their embryos were cultivated in Murashige and Skoog (MS) media containing plant growth regulators in various proportions. The viability rates of the seeds were found at 98.3% for A. orientalis and 96.0% for A. sieheana. Germination rates were determined only on filter paper as 8.3% for A. orientalis and 5.0% for A. sieheana, no germination was observed in other applications. The highest zygotic shoot and root formation rate were obtained from A. orientalis with 91.6% and 40.3%, respectively. But, the highest callus formation rate was observed on A. siehenea with 75.0%. As a result, it was determined that although the viability rates were high in both species, the germination rates were low, but in vitro reproducibility was possible.https://dergipark.org.tr/tr/pub/tutad/issue/62689/892928alkanna orientalisalkanna sieheanaalkannin/shikoninin vitrotetrazolium |
| spellingShingle | Cennet YAMAN Serkan URANBEY Hussein Abdullah Ahmed AHMED Dilek BAŞALMA Seed Viability Test, Germination Rate and In vitro Regeneration of Some Alkanna Species Türkiye Tarımsal Araştırmalar Dergisi alkanna orientalis alkanna sieheana alkannin/shikonin in vitro tetrazolium |
| title | Seed Viability Test, Germination Rate and In vitro Regeneration of Some Alkanna Species |
| title_full | Seed Viability Test, Germination Rate and In vitro Regeneration of Some Alkanna Species |
| title_fullStr | Seed Viability Test, Germination Rate and In vitro Regeneration of Some Alkanna Species |
| title_full_unstemmed | Seed Viability Test, Germination Rate and In vitro Regeneration of Some Alkanna Species |
| title_short | Seed Viability Test, Germination Rate and In vitro Regeneration of Some Alkanna Species |
| title_sort | seed viability test germination rate and in vitro regeneration of some alkanna species |
| topic | alkanna orientalis alkanna sieheana alkannin/shikonin in vitro tetrazolium |
| url | https://dergipark.org.tr/tr/pub/tutad/issue/62689/892928 |
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