Pyrazoles containing organic extracts of Litsea glutinosa (Lour.) C. B. Rob enervate chemical-induced diarrhea in animal models evident in ligand-receptor interaction
This research investigated the effect of organic extracts from Litsea glutinosa (Lour.) C. B. Rob bark and its five heterocyclic compounds on induced diarrheal models. The bark of L. glutinosa was extracted with chloroform, ethyl acetate, and methanol. The resultant extracts were examined for disc-d...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223003726 |
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| author | Md. Atiar Rahman Nazifa Anjum Md. Khalid Juhani Rafi Srabonti Saha Jobaier Ibne Deen Mijbah Uddin Farjana Sharmen Humayra Ferdousi Rahni Hossain |
| author_facet | Md. Atiar Rahman Nazifa Anjum Md. Khalid Juhani Rafi Srabonti Saha Jobaier Ibne Deen Mijbah Uddin Farjana Sharmen Humayra Ferdousi Rahni Hossain |
| author_sort | Md. Atiar Rahman |
| collection | DOAJ |
| description | This research investigated the effect of organic extracts from Litsea glutinosa (Lour.) C. B. Rob bark and its five heterocyclic compounds on induced diarrheal models. The bark of L. glutinosa was extracted with chloroform, ethyl acetate, and methanol. The resultant extracts were examined for disc-diffusion-guided activity against diarrhea-causing bacteria and chemical-induced anti-diarrheal properties in castor oil- and magnesium sulfate-induced diarrheal models. The effect of the extracts on gastrointestinal motility was tested in activated charcoal meal and barium sulfate milk models. The effects of the extracts on electrolytes (Na+, K+, Cl- and HCO3–), creatinine, triglycerides (TG), C-reactive protein (CRP), and immunoglobulin E (IgE) were assessed in the blood serum of treated animals. From the GC–MS analysis of the L. glutinosa methanol extract, five heterocyclic compounds were selected, and their interactions with target receptors were investigated using molecular docking techniques. The methanol extract (MExLG) showed the highest zone of inhibition for Shigella dysentriae (ZOI, 24 ± 0.9 mm) and E. coli (ZOI, 16.00 ± 1.14 mm), Salmonella paratyphi (18.23 ± 3.06 mm) and Vibrio cholerae (22.10 ± 2.62 mm). For both castor oil- and barium sulfate-induced diarrhea, MExLG achieved the highest levels of diarrheal inhibition 82.5% and 77.33%, respectively. MExLG showed the best in Na+, K+, Cl-, and HCO3– equivalence. Serum creatinine, TG, CRP and IgE levels were significantly (P < 0.05) restored by both MExLG and ethyl acetate extract (EAxLG). Out of five compounds, 1-(2-Fluorophenyl)pyrazole-4-carboxylic acid had the closest ligand-receptor interaction to that of the standard anti-diarrheal drug loperamide. The results demonstrate that the 1-(2-Fluorophenyl)pyrazole-4-carboxylic acid of MExLG could be positioned as a potential anti-diarrheal target through further affirmation in a dose–response cell-line study. |
| first_indexed | 2024-03-13T05:29:22Z |
| format | Article |
| id | doaj.art-5da7e88e239e4ae1a6888ffb4333b2b7 |
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| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-13T05:29:22Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
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| series | Arabian Journal of Chemistry |
| spelling | doaj.art-5da7e88e239e4ae1a6888ffb4333b2b72023-06-15T04:54:54ZengElsevierArabian Journal of Chemistry1878-53522023-08-01168104910Pyrazoles containing organic extracts of Litsea glutinosa (Lour.) C. B. Rob enervate chemical-induced diarrhea in animal models evident in ligand-receptor interactionMd. Atiar Rahman0Nazifa Anjum1Md. Khalid Juhani Rafi2Srabonti Saha3Jobaier Ibne Deen4Mijbah Uddin5Farjana Sharmen6Humayra Ferdousi7Rahni Hossain8Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, Bangladesh; School of Allied Health Sciences, Walailak University, Nakhon-Shi Thammarat, Nakhon Si Thammarat 80160, Thailand; Corresponding author at: International Professor (Distinguished Scholar Category), Walailak University, Nakhon Si Thammarat 80160, Thailand, Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong 4331, Bangladesh.Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, BangladeshDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, BangladeshDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, BangladeshDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, BangladeshDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, BangladeshDepartment of Genetic Engineering and Biotechnology, University of Chittagong, Chittagong 4331, BangladeshDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, BangladeshSchool of Allied Health Sciences, Walailak University, Nakhon-Shi Thammarat, Nakhon Si Thammarat 80160, ThailandThis research investigated the effect of organic extracts from Litsea glutinosa (Lour.) C. B. Rob bark and its five heterocyclic compounds on induced diarrheal models. The bark of L. glutinosa was extracted with chloroform, ethyl acetate, and methanol. The resultant extracts were examined for disc-diffusion-guided activity against diarrhea-causing bacteria and chemical-induced anti-diarrheal properties in castor oil- and magnesium sulfate-induced diarrheal models. The effect of the extracts on gastrointestinal motility was tested in activated charcoal meal and barium sulfate milk models. The effects of the extracts on electrolytes (Na+, K+, Cl- and HCO3–), creatinine, triglycerides (TG), C-reactive protein (CRP), and immunoglobulin E (IgE) were assessed in the blood serum of treated animals. From the GC–MS analysis of the L. glutinosa methanol extract, five heterocyclic compounds were selected, and their interactions with target receptors were investigated using molecular docking techniques. The methanol extract (MExLG) showed the highest zone of inhibition for Shigella dysentriae (ZOI, 24 ± 0.9 mm) and E. coli (ZOI, 16.00 ± 1.14 mm), Salmonella paratyphi (18.23 ± 3.06 mm) and Vibrio cholerae (22.10 ± 2.62 mm). For both castor oil- and barium sulfate-induced diarrhea, MExLG achieved the highest levels of diarrheal inhibition 82.5% and 77.33%, respectively. MExLG showed the best in Na+, K+, Cl-, and HCO3– equivalence. Serum creatinine, TG, CRP and IgE levels were significantly (P < 0.05) restored by both MExLG and ethyl acetate extract (EAxLG). Out of five compounds, 1-(2-Fluorophenyl)pyrazole-4-carboxylic acid had the closest ligand-receptor interaction to that of the standard anti-diarrheal drug loperamide. The results demonstrate that the 1-(2-Fluorophenyl)pyrazole-4-carboxylic acid of MExLG could be positioned as a potential anti-diarrheal target through further affirmation in a dose–response cell-line study.http://www.sciencedirect.com/science/article/pii/S1878535223003726Litsea glutinosaDiarrhea-causing bacteriaC-reactive proteinIgE1-(2-Fluorophenyl)pyrazole-4-carboxylic acid |
| spellingShingle | Md. Atiar Rahman Nazifa Anjum Md. Khalid Juhani Rafi Srabonti Saha Jobaier Ibne Deen Mijbah Uddin Farjana Sharmen Humayra Ferdousi Rahni Hossain Pyrazoles containing organic extracts of Litsea glutinosa (Lour.) C. B. Rob enervate chemical-induced diarrhea in animal models evident in ligand-receptor interaction Arabian Journal of Chemistry Litsea glutinosa Diarrhea-causing bacteria C-reactive protein IgE 1-(2-Fluorophenyl)pyrazole-4-carboxylic acid |
| title | Pyrazoles containing organic extracts of Litsea glutinosa (Lour.) C. B. Rob enervate chemical-induced diarrhea in animal models evident in ligand-receptor interaction |
| title_full | Pyrazoles containing organic extracts of Litsea glutinosa (Lour.) C. B. Rob enervate chemical-induced diarrhea in animal models evident in ligand-receptor interaction |
| title_fullStr | Pyrazoles containing organic extracts of Litsea glutinosa (Lour.) C. B. Rob enervate chemical-induced diarrhea in animal models evident in ligand-receptor interaction |
| title_full_unstemmed | Pyrazoles containing organic extracts of Litsea glutinosa (Lour.) C. B. Rob enervate chemical-induced diarrhea in animal models evident in ligand-receptor interaction |
| title_short | Pyrazoles containing organic extracts of Litsea glutinosa (Lour.) C. B. Rob enervate chemical-induced diarrhea in animal models evident in ligand-receptor interaction |
| title_sort | pyrazoles containing organic extracts of litsea glutinosa lour c b rob enervate chemical induced diarrhea in animal models evident in ligand receptor interaction |
| topic | Litsea glutinosa Diarrhea-causing bacteria C-reactive protein IgE 1-(2-Fluorophenyl)pyrazole-4-carboxylic acid |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223003726 |
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