Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B<sub>12</sub> Derivatives
This work demonstrates resonance Raman optical activity (RROA) spectra of three truncated vitamin B<sub>12</sub> derivatives modified within the nucleotide loop. Since truncated cobalamins possess sufficiently high rotational strength in the range of ROA excitation (532 nm), it was possi...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/19/4386 |
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| author | Ewa Machalska Grzegorz Zajac Monika Halat Aleksandra J. Wierzba Dorota Gryko Malgorzata Baranska |
| author_facet | Ewa Machalska Grzegorz Zajac Monika Halat Aleksandra J. Wierzba Dorota Gryko Malgorzata Baranska |
| author_sort | Ewa Machalska |
| collection | DOAJ |
| description | This work demonstrates resonance Raman optical activity (RROA) spectra of three truncated vitamin B<sub>12</sub> derivatives modified within the nucleotide loop. Since truncated cobalamins possess sufficiently high rotational strength in the range of ROA excitation (532 nm), it was possible to record their spectra in the resonance condition. They showed several distinct spectral features allowing for the distinguishing of studied compounds, in contrast to other methods, i.e., UV-Vis absorption, electronic circular dichroism, and resonance Raman spectroscopy. The improved capacity of the RROA method is based here on the excitation of molecules via more than two electronic states, giving rise to the bisignate RROA spectrum, significantly distinct from a parent Raman spectrum. This observation is an important step in the dissemination of using RROA spectroscopy in studying the complex structure of corrinoids which may prove crucial for a better understanding of their biological role. |
| first_indexed | 2024-03-10T16:05:37Z |
| format | Article |
| id | doaj.art-5daa20edb9704dc58d4bd0b528cc2790 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:05:37Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5daa20edb9704dc58d4bd0b528cc27902023-11-20T14:54:14ZengMDPI AGMolecules1420-30492020-09-012519438610.3390/molecules25194386Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B<sub>12</sub> DerivativesEwa Machalska0Grzegorz Zajac1Monika Halat2Aleksandra J. Wierzba3Dorota Gryko4Malgorzata Baranska5Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandJagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, PolandFaculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandInstitute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, PolandInstitute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, PolandFaculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandThis work demonstrates resonance Raman optical activity (RROA) spectra of three truncated vitamin B<sub>12</sub> derivatives modified within the nucleotide loop. Since truncated cobalamins possess sufficiently high rotational strength in the range of ROA excitation (532 nm), it was possible to record their spectra in the resonance condition. They showed several distinct spectral features allowing for the distinguishing of studied compounds, in contrast to other methods, i.e., UV-Vis absorption, electronic circular dichroism, and resonance Raman spectroscopy. The improved capacity of the RROA method is based here on the excitation of molecules via more than two electronic states, giving rise to the bisignate RROA spectrum, significantly distinct from a parent Raman spectrum. This observation is an important step in the dissemination of using RROA spectroscopy in studying the complex structure of corrinoids which may prove crucial for a better understanding of their biological role.https://www.mdpi.com/1420-3049/25/19/4386vitamin B<sub>12</sub>cobinamidechiralityresonance ROAresonance Raman spectroscopyECD |
| spellingShingle | Ewa Machalska Grzegorz Zajac Monika Halat Aleksandra J. Wierzba Dorota Gryko Malgorzata Baranska Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B<sub>12</sub> Derivatives Molecules vitamin B<sub>12</sub> cobinamide chirality resonance ROA resonance Raman spectroscopy ECD |
| title | Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B<sub>12</sub> Derivatives |
| title_full | Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B<sub>12</sub> Derivatives |
| title_fullStr | Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B<sub>12</sub> Derivatives |
| title_full_unstemmed | Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B<sub>12</sub> Derivatives |
| title_short | Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B<sub>12</sub> Derivatives |
| title_sort | resonance raman optical activity spectroscopy in probing structural changes invisible to circular dichroism spectroscopy a study on truncated vitamin b sub 12 sub derivatives |
| topic | vitamin B<sub>12</sub> cobinamide chirality resonance ROA resonance Raman spectroscopy ECD |
| url | https://www.mdpi.com/1420-3049/25/19/4386 |
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