Community context and pCO2 impact the transcriptome of the “helper” bacterium Alteromonas in co-culture with picocyanobacteria
Abstract Many microbial photoautotrophs depend on heterotrophic bacteria for accomplishing essential functions. Environmental changes, however, could alter or eliminate such interactions. We investigated the effects of changing pCO2 on gene transcription in co-cultures of 3 strains of picocyanobacte...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press
2022-11-01
|
| Series: | ISME Communications |
| Online Access: | https://doi.org/10.1038/s43705-022-00197-2 |
| _version_ | 1797228908322488320 |
|---|---|
| author | Marcelo Malisano Barreto Filho Zhiying Lu Melissa Walker J. Jeffrey Morris |
| author_facet | Marcelo Malisano Barreto Filho Zhiying Lu Melissa Walker J. Jeffrey Morris |
| author_sort | Marcelo Malisano Barreto Filho |
| collection | DOAJ |
| description | Abstract Many microbial photoautotrophs depend on heterotrophic bacteria for accomplishing essential functions. Environmental changes, however, could alter or eliminate such interactions. We investigated the effects of changing pCO2 on gene transcription in co-cultures of 3 strains of picocyanobacteria (Synechococcus strains CC9311 and WH8102 and Prochlorococcus strain MIT9312) paired with the ‘helper’ bacterium Alteromonas macleodii EZ55. Co-culture with cyanobacteria resulted in a much higher number of up- and down-regulated genes in EZ55 than pCO2 by itself. Pathway analysis revealed significantly different transcription of genes involved in carbohydrate metabolism, stress response, and chemotaxis, with different patterns of up- or down-regulation in co-culture with different cyanobacterial strains. Gene transcription patterns of organic and inorganic nutrient transporter and catabolism genes in EZ55 suggested resources available in the culture media were altered under elevated (800 ppm) pCO2 conditions. Altogether, changing transcription patterns were consistent with the possibility that the composition of cyanobacterial excretions changed under the two pCO2 regimes, causing extensive ecophysiological changes in both members of the co-cultures. Additionally, significant downregulation of oxidative stress genes in MIT9312/EZ55 cocultures at 800 ppm pCO2 were consistent with a link between the predicted reduced availability of photorespiratory byproducts (i.e., glycolate/2PG) under this condition and observed reductions in internal oxidative stress loads for EZ55, providing a possible explanation for the previously observed lack of “help” provided by EZ55 to MIT9312 under elevated pCO2. If similar broad alterations in microbial ecophysiology occur in the ocean as atmospheric pCO2 increases, they could lead to substantially altered ecosystem functioning and community composition. |
| first_indexed | 2024-04-24T15:04:10Z |
| format | Article |
| id | doaj.art-5daa21be3877460c8f6fb1366f9c77fa |
| institution | Directory Open Access Journal |
| issn | 2730-6151 |
| language | English |
| last_indexed | 2024-04-24T15:04:10Z |
| publishDate | 2022-11-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | ISME Communications |
| spelling | doaj.art-5daa21be3877460c8f6fb1366f9c77fa2024-04-02T13:58:53ZengOxford University PressISME Communications2730-61512022-11-012111110.1038/s43705-022-00197-2Community context and pCO2 impact the transcriptome of the “helper” bacterium Alteromonas in co-culture with picocyanobacteriaMarcelo Malisano Barreto Filho0Zhiying Lu1Melissa Walker2J. Jeffrey Morris3University of Alabama at Birmingham Department of Biology, 1300 University Blvd CH464University of Alabama at Birmingham Department of Biology, 1300 University Blvd CH464University of Alabama at Birmingham Department of Biology, 1300 University Blvd CH464University of Alabama at Birmingham Department of Biology, 1300 University Blvd CH464Abstract Many microbial photoautotrophs depend on heterotrophic bacteria for accomplishing essential functions. Environmental changes, however, could alter or eliminate such interactions. We investigated the effects of changing pCO2 on gene transcription in co-cultures of 3 strains of picocyanobacteria (Synechococcus strains CC9311 and WH8102 and Prochlorococcus strain MIT9312) paired with the ‘helper’ bacterium Alteromonas macleodii EZ55. Co-culture with cyanobacteria resulted in a much higher number of up- and down-regulated genes in EZ55 than pCO2 by itself. Pathway analysis revealed significantly different transcription of genes involved in carbohydrate metabolism, stress response, and chemotaxis, with different patterns of up- or down-regulation in co-culture with different cyanobacterial strains. Gene transcription patterns of organic and inorganic nutrient transporter and catabolism genes in EZ55 suggested resources available in the culture media were altered under elevated (800 ppm) pCO2 conditions. Altogether, changing transcription patterns were consistent with the possibility that the composition of cyanobacterial excretions changed under the two pCO2 regimes, causing extensive ecophysiological changes in both members of the co-cultures. Additionally, significant downregulation of oxidative stress genes in MIT9312/EZ55 cocultures at 800 ppm pCO2 were consistent with a link between the predicted reduced availability of photorespiratory byproducts (i.e., glycolate/2PG) under this condition and observed reductions in internal oxidative stress loads for EZ55, providing a possible explanation for the previously observed lack of “help” provided by EZ55 to MIT9312 under elevated pCO2. If similar broad alterations in microbial ecophysiology occur in the ocean as atmospheric pCO2 increases, they could lead to substantially altered ecosystem functioning and community composition.https://doi.org/10.1038/s43705-022-00197-2 |
| spellingShingle | Marcelo Malisano Barreto Filho Zhiying Lu Melissa Walker J. Jeffrey Morris Community context and pCO2 impact the transcriptome of the “helper” bacterium Alteromonas in co-culture with picocyanobacteria ISME Communications |
| title | Community context and pCO2 impact the transcriptome of the “helper” bacterium Alteromonas in co-culture with picocyanobacteria |
| title_full | Community context and pCO2 impact the transcriptome of the “helper” bacterium Alteromonas in co-culture with picocyanobacteria |
| title_fullStr | Community context and pCO2 impact the transcriptome of the “helper” bacterium Alteromonas in co-culture with picocyanobacteria |
| title_full_unstemmed | Community context and pCO2 impact the transcriptome of the “helper” bacterium Alteromonas in co-culture with picocyanobacteria |
| title_short | Community context and pCO2 impact the transcriptome of the “helper” bacterium Alteromonas in co-culture with picocyanobacteria |
| title_sort | community context and pco2 impact the transcriptome of the helper bacterium alteromonas in co culture with picocyanobacteria |
| url | https://doi.org/10.1038/s43705-022-00197-2 |
| work_keys_str_mv | AT marcelomalisanobarretofilho communitycontextandpco2impactthetranscriptomeofthehelperbacteriumalteromonasincoculturewithpicocyanobacteria AT zhiyinglu communitycontextandpco2impactthetranscriptomeofthehelperbacteriumalteromonasincoculturewithpicocyanobacteria AT melissawalker communitycontextandpco2impactthetranscriptomeofthehelperbacteriumalteromonasincoculturewithpicocyanobacteria AT jjeffreymorris communitycontextandpco2impactthetranscriptomeofthehelperbacteriumalteromonasincoculturewithpicocyanobacteria |