Anxiolytic effects of Formononetin in an inflammatory pain mouse model
Abstract Chronic pain is commonly accompanied with anxiety disorder, which complicates treatment. In this study, we investigated the analgesic and anxiolytic effects of Formononetin (FMNT), an active component of traditional Chinese medicine red clover (Trifolium pratense L.) that is capable of prot...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-04-01
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| Series: | Molecular Brain |
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| Online Access: | http://link.springer.com/article/10.1186/s13041-019-0453-4 |
| _version_ | 1818151429567676416 |
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| author | Xin-shang Wang Shao-yu Guan An Liu Jiao Yue Li-ning Hu Kun Zhang Liu-kun Yang Liang Lu Zhen Tian Ming-gao Zhao Shui-bing Liu |
| author_facet | Xin-shang Wang Shao-yu Guan An Liu Jiao Yue Li-ning Hu Kun Zhang Liu-kun Yang Liang Lu Zhen Tian Ming-gao Zhao Shui-bing Liu |
| author_sort | Xin-shang Wang |
| collection | DOAJ |
| description | Abstract Chronic pain is commonly accompanied with anxiety disorder, which complicates treatment. In this study, we investigated the analgesic and anxiolytic effects of Formononetin (FMNT), an active component of traditional Chinese medicine red clover (Trifolium pratense L.) that is capable of protecting neurons from N-methyl-D-aspartate (NMDA)-evoked excitotoxic injury, on mice suffering from complete Freund’s adjuvant (CFA)-induced chronic inflammatory pain. The results show that FMNT administration significantly reduces anxiety-like behavior but does not affect the nociceptive threshold in CFA-injected mice. The treatment reverses the upregulation of NMDA, GluA1, and GABAA receptors, as well as PSD95 and CREB in the basolateral amygdala (BLA). The effects of FMNT on NMDA receptors and CREB binding protein (CBP) were further confirmed by the potential structure combination between these compounds, which was analyzed by in silico docking technology. FMNT also inhibits the activation of the NF-κB signaling pathway and microglia in the BLA of mice suffering from chronic inflammatory pain. Therefore, the anxiolytic effects of FMNT are partially due to the attenuation of inflammation and neuronal hyperexcitability through the inhibition of NMDA receptor and CBP in the BLA. |
| first_indexed | 2024-12-11T13:38:41Z |
| format | Article |
| id | doaj.art-5dab2c23030e42e994a7d3a9174d089e |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-12-11T13:38:41Z |
| publishDate | 2019-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-5dab2c23030e42e994a7d3a9174d089e2022-12-22T01:04:54ZengBMCMolecular Brain1756-66062019-04-0112111210.1186/s13041-019-0453-4Anxiolytic effects of Formononetin in an inflammatory pain mouse modelXin-shang Wang0Shao-yu Guan1An Liu2Jiao Yue3Li-ning Hu4Kun Zhang5Liu-kun Yang6Liang Lu7Zhen Tian8Ming-gao Zhao9Shui-bing Liu10Department of Pharmacology, School of Pharmacy, and Precision Pharmacy & Drug Development Center, Department of Pharmacy, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Pharmacology, School of Pharmacy, and Precision Pharmacy & Drug Development Center, Department of Pharmacy, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Pharmacology, School of Pharmacy, and Precision Pharmacy & Drug Development Center, Department of Pharmacy, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Pharmacology, School of Pharmacy, and Precision Pharmacy & Drug Development Center, Department of Pharmacy, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Pharmacology, School of Pharmacy, and Precision Pharmacy & Drug Development Center, Department of Pharmacy, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Pharmacology, School of Pharmacy, and Precision Pharmacy & Drug Development Center, Department of Pharmacy, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Pharmacology, School of Pharmacy, and Precision Pharmacy & Drug Development Center, Department of Pharmacy, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Pharmacology, School of Pharmacy, and Precision Pharmacy & Drug Development Center, Department of Pharmacy, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Pharmacology, School of Pharmacy, and Precision Pharmacy & Drug Development Center, Department of Pharmacy, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Pharmacology, School of Pharmacy, and Precision Pharmacy & Drug Development Center, Department of Pharmacy, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Pharmacology, School of Pharmacy, and Precision Pharmacy & Drug Development Center, Department of Pharmacy, Tangdu Hospital, Fourth Military Medical UniversityAbstract Chronic pain is commonly accompanied with anxiety disorder, which complicates treatment. In this study, we investigated the analgesic and anxiolytic effects of Formononetin (FMNT), an active component of traditional Chinese medicine red clover (Trifolium pratense L.) that is capable of protecting neurons from N-methyl-D-aspartate (NMDA)-evoked excitotoxic injury, on mice suffering from complete Freund’s adjuvant (CFA)-induced chronic inflammatory pain. The results show that FMNT administration significantly reduces anxiety-like behavior but does not affect the nociceptive threshold in CFA-injected mice. The treatment reverses the upregulation of NMDA, GluA1, and GABAA receptors, as well as PSD95 and CREB in the basolateral amygdala (BLA). The effects of FMNT on NMDA receptors and CREB binding protein (CBP) were further confirmed by the potential structure combination between these compounds, which was analyzed by in silico docking technology. FMNT also inhibits the activation of the NF-κB signaling pathway and microglia in the BLA of mice suffering from chronic inflammatory pain. Therefore, the anxiolytic effects of FMNT are partially due to the attenuation of inflammation and neuronal hyperexcitability through the inhibition of NMDA receptor and CBP in the BLA.http://link.springer.com/article/10.1186/s13041-019-0453-4InflammationChronic painAnxietyFormononetinAmygdalaNMDA |
| spellingShingle | Xin-shang Wang Shao-yu Guan An Liu Jiao Yue Li-ning Hu Kun Zhang Liu-kun Yang Liang Lu Zhen Tian Ming-gao Zhao Shui-bing Liu Anxiolytic effects of Formononetin in an inflammatory pain mouse model Molecular Brain Inflammation Chronic pain Anxiety Formononetin Amygdala NMDA |
| title | Anxiolytic effects of Formononetin in an inflammatory pain mouse model |
| title_full | Anxiolytic effects of Formononetin in an inflammatory pain mouse model |
| title_fullStr | Anxiolytic effects of Formononetin in an inflammatory pain mouse model |
| title_full_unstemmed | Anxiolytic effects of Formononetin in an inflammatory pain mouse model |
| title_short | Anxiolytic effects of Formononetin in an inflammatory pain mouse model |
| title_sort | anxiolytic effects of formononetin in an inflammatory pain mouse model |
| topic | Inflammation Chronic pain Anxiety Formononetin Amygdala NMDA |
| url | http://link.springer.com/article/10.1186/s13041-019-0453-4 |
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