Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-<i>co</i>-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning Techniques
We developed biodegradable drug-eluting prolapse mats using solution-extrusion 3D printing and coaxial electrospinning techniques. The mats were composed of polycaprolactone (PCL) mesh and lidocaine-, estradiol-, metronidazole-, and connective tissue growth factor (CTGF)-incorporated poly(lactic-<...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/14/2295 |
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| author | Yi-Pin Chen Tsia-Shu Lo Yu-Ting Lin Yu-Han Chien Chia-Jung Lu Shih-Jung Liu |
| author_facet | Yi-Pin Chen Tsia-Shu Lo Yu-Ting Lin Yu-Han Chien Chia-Jung Lu Shih-Jung Liu |
| author_sort | Yi-Pin Chen |
| collection | DOAJ |
| description | We developed biodegradable drug-eluting prolapse mats using solution-extrusion 3D printing and coaxial electrospinning techniques. The mats were composed of polycaprolactone (PCL) mesh and lidocaine-, estradiol-, metronidazole-, and connective tissue growth factor (CTGF)-incorporated poly(lactic-<i>co</i>-glycolic acid) (PLGA) nanofibers that mimic the structure of the natural extracellular matrix of most connective tissues. The mechanical properties of degradable prolapse membrane were assessed and compared to commercial non-degradable polypropylene knitted meshes clinically used for pelvic organ prolapse (POP) repair. The release behaviors of the drug-loaded hybrid degradable membranes were also characterized. The experimental results suggest that 3D-printed PCL meshes exhibited comparable strengths to commercial POP meshes and survived through 10,000 cycles of fatigue test without breakage. Hybrid PCL meshes/PLGA nanofibrous membranes provided a sustainable release of metronidazole, lidocaine, and estradiol for 4, 25, and 30 days, respectively, in vitro. The membranes further liberated high levels of CTGF for more than 30 days. The animal tests show that the mechanical property of PCL mesh decreased with time, mainly due to degradation of the polymers post-implantation. No adverse effect of the mesh/nanofibers was noted in the histological images. By adopting solution-extrusion 3D printing and coaxial electrospinning, degradable drug-eluting membranes can be fabricated for POP applications. |
| first_indexed | 2024-03-10T09:27:31Z |
| format | Article |
| id | doaj.art-5db0dcbbe6aa49c4aef91b9ea9b6b7bd |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T09:27:31Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-5db0dcbbe6aa49c4aef91b9ea9b6b7bd2023-11-22T04:46:06ZengMDPI AGPolymers2073-43602021-07-011314229510.3390/polym13142295Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-<i>co</i>-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning TechniquesYi-Pin Chen0Tsia-Shu Lo1Yu-Ting Lin2Yu-Han Chien3Chia-Jung Lu4Shih-Jung Liu5Department of Obstetrics and Gynecology, Keelung Chang Gung Memorial Hospital, Keelung 20401, TaiwanDepartment of Obstetrics and Gynecology, Chang Gung Memorial Hospital-Linkou, School of Medicine, Chang Gung University, Taoyuan 33305, TaiwanDepartment of Mechanical Engineering, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Mechanical Engineering, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Mechanical Engineering, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Mechanical Engineering, Chang Gung University, Taoyuan 33302, TaiwanWe developed biodegradable drug-eluting prolapse mats using solution-extrusion 3D printing and coaxial electrospinning techniques. The mats were composed of polycaprolactone (PCL) mesh and lidocaine-, estradiol-, metronidazole-, and connective tissue growth factor (CTGF)-incorporated poly(lactic-<i>co</i>-glycolic acid) (PLGA) nanofibers that mimic the structure of the natural extracellular matrix of most connective tissues. The mechanical properties of degradable prolapse membrane were assessed and compared to commercial non-degradable polypropylene knitted meshes clinically used for pelvic organ prolapse (POP) repair. The release behaviors of the drug-loaded hybrid degradable membranes were also characterized. The experimental results suggest that 3D-printed PCL meshes exhibited comparable strengths to commercial POP meshes and survived through 10,000 cycles of fatigue test without breakage. Hybrid PCL meshes/PLGA nanofibrous membranes provided a sustainable release of metronidazole, lidocaine, and estradiol for 4, 25, and 30 days, respectively, in vitro. The membranes further liberated high levels of CTGF for more than 30 days. The animal tests show that the mechanical property of PCL mesh decreased with time, mainly due to degradation of the polymers post-implantation. No adverse effect of the mesh/nanofibers was noted in the histological images. By adopting solution-extrusion 3D printing and coaxial electrospinning, degradable drug-eluting membranes can be fabricated for POP applications.https://www.mdpi.com/2073-4360/13/14/2295prolapse membranesolution-extrusion 3D printingcoaxial electrospinningpolycaprolactonepoly(lactic-<i>co</i>-glycolic acid)nanofibers |
| spellingShingle | Yi-Pin Chen Tsia-Shu Lo Yu-Ting Lin Yu-Han Chien Chia-Jung Lu Shih-Jung Liu Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-<i>co</i>-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning Techniques Polymers prolapse membrane solution-extrusion 3D printing coaxial electrospinning polycaprolactone poly(lactic-<i>co</i>-glycolic acid) nanofibers |
| title | Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-<i>co</i>-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning Techniques |
| title_full | Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-<i>co</i>-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning Techniques |
| title_fullStr | Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-<i>co</i>-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning Techniques |
| title_full_unstemmed | Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-<i>co</i>-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning Techniques |
| title_short | Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-<i>co</i>-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning Techniques |
| title_sort | fabrication of drug eluting polycaprolactone poly lactic i co i glycolic acid prolapse mats using solution extrusion 3d printing and coaxial electrospinning techniques |
| topic | prolapse membrane solution-extrusion 3D printing coaxial electrospinning polycaprolactone poly(lactic-<i>co</i>-glycolic acid) nanofibers |
| url | https://www.mdpi.com/2073-4360/13/14/2295 |
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