Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India)
Microorganisms capable of using naphthalene as the sole carbon source were isolated from the contaminated sediment of Cooum River. Twenty one isolates were recovered and nine were selected for enrichment due to differences in their morphological characteristics. Out of nine isolates, only four (NS3-...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2019-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2019/0352-51391800088R.pdf |
| _version_ | 1818807578327515136 |
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| author | Rajan Sancho Pattanaik Anwesha Kumaresan Venkatesh Bhatt Prasanth Gunasekaran Senthilarasu Arockiaraj Jesu Pasupuleti Mukesh Beskoski Vladimir P. Chakraborty Paromita |
| author_facet | Rajan Sancho Pattanaik Anwesha Kumaresan Venkatesh Bhatt Prasanth Gunasekaran Senthilarasu Arockiaraj Jesu Pasupuleti Mukesh Beskoski Vladimir P. Chakraborty Paromita |
| author_sort | Rajan Sancho |
| collection | DOAJ |
| description | Microorganisms capable of using naphthalene as the sole carbon source were isolated from the contaminated sediment of Cooum River. Twenty one isolates were recovered and nine were selected for enrichment due to differences in their morphological characteristics. Out of nine isolates, only four (NS3-SRMND14B, NS14-SRMND14A, NS15-SRMND14D and NS19- -SRMND14E) were capable of completely utilizing naphthalene as the sole source of carbon in carbon free minimal medium (CFMM) supplemented with naphthalene. 16S rDNA sequencing showed that all the four isolates were distantly related to each other and belongs to Bacillus sp. (NS3-SRMND14B), Pseudomonas sp. (NS14-SRMND14A), Cellulosimicrobium sp. (NS15-SRMND14D) and Sphingobacterium sp. (NS19-SRMND14E), respectively. Based on the phylogenetic analysis of 16S rDNA sequencing, the isolate Sphingobacterium sp. (NS19-SRMND14E) has been identified as novel strain. Polymerase chain reaction (PCR) technique showed the presence of naphthalene dioxygenase (ndo) gene responsible for naphthalene degradation only in the Pseudomonas sp. (NS14-SRMND14A). We observed that the ndo gene is not the only gene responsible for naphthalene degradation. Based on our study, the indigenous microorganisms isolated from Cooum Riverine sediment can be used for bioremediation of the polluted sediment along the Bay of Bengal. |
| first_indexed | 2024-12-18T19:27:53Z |
| format | Article |
| id | doaj.art-5db196ff9d974b46baa66c17fa684fc5 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-12-18T19:27:53Z |
| publishDate | 2019-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-5db196ff9d974b46baa66c17fa684fc52022-12-21T20:55:48ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212019-01-0184222523610.2298/JSC180724088R0352-51391800088RCharacterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India)Rajan Sancho0Pattanaik Anwesha1Kumaresan Venkatesh2Bhatt Prasanth3Gunasekaran Senthilarasu4Arockiaraj Jesu5Pasupuleti Mukesh6Beskoski Vladimir P.7Chakraborty Paromita8SRM Institute of Science and Technology, Department of Civil Engineering, Kattankulathur, Tamil Nadu, IndiaSRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, IndiaSRM Institute of Science and Technology, Faculty of Science and Humanities, Department of Biotechnology, Division of Fisheries Biotechnology & Molecular Biology, Kattankulathur, Tamil Nadu, IndiaSRM Institute of Science and Technology, Faculty of Science and Humanities, Department of Biotechnology, Division of Fisheries Biotechnology & Molecular Biology, Kattankulathur, Tamil Nadu, IndiaSRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, IndiaSRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, IndiaCentral Drug Research Institute, B.S. 10/1, Sector 10, Jankipuram Extension, Lucknow, IndiaFaculty of Chemistry, BelgradeSRM Institute of Science and Technology, Department of Civil Engineering, Kattankulathur, Tamil Nadu, India + SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, IndiaMicroorganisms capable of using naphthalene as the sole carbon source were isolated from the contaminated sediment of Cooum River. Twenty one isolates were recovered and nine were selected for enrichment due to differences in their morphological characteristics. Out of nine isolates, only four (NS3-SRMND14B, NS14-SRMND14A, NS15-SRMND14D and NS19- -SRMND14E) were capable of completely utilizing naphthalene as the sole source of carbon in carbon free minimal medium (CFMM) supplemented with naphthalene. 16S rDNA sequencing showed that all the four isolates were distantly related to each other and belongs to Bacillus sp. (NS3-SRMND14B), Pseudomonas sp. (NS14-SRMND14A), Cellulosimicrobium sp. (NS15-SRMND14D) and Sphingobacterium sp. (NS19-SRMND14E), respectively. Based on the phylogenetic analysis of 16S rDNA sequencing, the isolate Sphingobacterium sp. (NS19-SRMND14E) has been identified as novel strain. Polymerase chain reaction (PCR) technique showed the presence of naphthalene dioxygenase (ndo) gene responsible for naphthalene degradation only in the Pseudomonas sp. (NS14-SRMND14A). We observed that the ndo gene is not the only gene responsible for naphthalene degradation. Based on our study, the indigenous microorganisms isolated from Cooum Riverine sediment can be used for bioremediation of the polluted sediment along the Bay of Bengal.http://www.doiserbia.nb.rs/img/doi/0352-5139/2019/0352-51391800088R.pdfnaphthaleneindigenous bacteriando gene16s RNAremediationphylogenetic tree |
| spellingShingle | Rajan Sancho Pattanaik Anwesha Kumaresan Venkatesh Bhatt Prasanth Gunasekaran Senthilarasu Arockiaraj Jesu Pasupuleti Mukesh Beskoski Vladimir P. Chakraborty Paromita Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India) Journal of the Serbian Chemical Society naphthalene indigenous bacteria ndo gene 16s RNA remediation phylogenetic tree |
| title | Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India) |
| title_full | Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India) |
| title_fullStr | Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India) |
| title_full_unstemmed | Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India) |
| title_short | Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India) |
| title_sort | characterization of some naphthalene using bacteria isolated from contaminated cooum riverine sediment of the bay of bengal india |
| topic | naphthalene indigenous bacteria ndo gene 16s RNA remediation phylogenetic tree |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2019/0352-51391800088R.pdf |
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