The Antioxidant Activities of <i>Betula etnensis</i> Rafin. Ethanolic Extract Exert Protective and Anti-diabetic Effects on Streptozotocin-Induced Diabetes in Rats
Pathophysiological mechanisms correlating diabetes mellitus with associated complications are still not completely clear, even though oxidative stress seems to play a pivotal role. Literature data suggest that cell damages induced by hyperglycemia, although multifactorial, have a common pathway in o...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/9/847 |
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| author | Giuseppe Antonio Malfa Barbara Tomasello Rosaria Acquaviva Alfonsina La Mantia Francesco Pappalardo Monica Ragusa Marcella Renis Claudia Di Giacomo |
| author_facet | Giuseppe Antonio Malfa Barbara Tomasello Rosaria Acquaviva Alfonsina La Mantia Francesco Pappalardo Monica Ragusa Marcella Renis Claudia Di Giacomo |
| author_sort | Giuseppe Antonio Malfa |
| collection | DOAJ |
| description | Pathophysiological mechanisms correlating diabetes mellitus with associated complications are still not completely clear, even though oxidative stress seems to play a pivotal role. Literature data suggest that cell damages induced by hyperglycemia, although multifactorial, have a common pathway in oxidative/nitrosative stress. The present study evaluated the effects of <i>Betula etnensis</i> Raf. bark extract, a plant belonging to the Betulaceae family endemic to Sicily, on oxidative stress and in preventing and/or retarding diabetes-associated complications in streptozotocin diabetic rats treated with the extract at dose of 0.5 g/kg body weight per day for 28 consecutive days. The extract administration significant decreased food and water intake, fasting blood glucose, weight loss and polyuria, compared with untreated diabetic animals. Furthermore, oxidative stress markers particularly, lipid hydroperoxides (LOOH) and nitrite/nitrate levels, non-proteic thiol groups (RSH), γ-glutamyl-cysteine-synthetase (γ-GCS) activities and expression, heme oxygenase-1 (HO-1), endothelial and inducible nitric oxide synthases (i-NOS e-NOS) expression, significantly changed by streptozocin treatment, were markedly restored both in plasma and tissues together with nuclear sirtuins activity (Sirt1). Results suggested that <i>B. etnensis</i> bark alcoholic extract is able to counteract oxidative stress and to ameliorate some general parameters related to diabetes. |
| first_indexed | 2024-03-10T16:26:23Z |
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| id | doaj.art-5db4e830beb945e4bf890e1735381a36 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T16:26:23Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-5db4e830beb945e4bf890e1735381a362023-11-20T13:10:49ZengMDPI AGAntioxidants2076-39212020-09-019984710.3390/antiox9090847The Antioxidant Activities of <i>Betula etnensis</i> Rafin. Ethanolic Extract Exert Protective and Anti-diabetic Effects on Streptozotocin-Induced Diabetes in RatsGiuseppe Antonio Malfa0Barbara Tomasello1Rosaria Acquaviva2Alfonsina La Mantia3Francesco Pappalardo4Monica Ragusa5Marcella Renis6Claudia Di Giacomo7Department of Drug Science, Section of Biochemistry, University of Catania, Viale A. Doria 6, 95125 Catania, ItalyDepartment of Drug Science, Section of Biochemistry, University of Catania, Viale A. Doria 6, 95125 Catania, ItalyDepartment of Drug Science, Section of Biochemistry, University of Catania, Viale A. Doria 6, 95125 Catania, ItalyDepartment of Drug Science, Section of Biochemistry, University of Catania, Viale A. Doria 6, 95125 Catania, ItalyDepartment of Drug Science, Section of Biochemistry, University of Catania, Viale A. Doria 6, 95125 Catania, ItalyDepartment of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Drug Science, Section of Biochemistry, University of Catania, Viale A. Doria 6, 95125 Catania, ItalyDepartment of Drug Science, Section of Biochemistry, University of Catania, Viale A. Doria 6, 95125 Catania, ItalyPathophysiological mechanisms correlating diabetes mellitus with associated complications are still not completely clear, even though oxidative stress seems to play a pivotal role. Literature data suggest that cell damages induced by hyperglycemia, although multifactorial, have a common pathway in oxidative/nitrosative stress. The present study evaluated the effects of <i>Betula etnensis</i> Raf. bark extract, a plant belonging to the Betulaceae family endemic to Sicily, on oxidative stress and in preventing and/or retarding diabetes-associated complications in streptozotocin diabetic rats treated with the extract at dose of 0.5 g/kg body weight per day for 28 consecutive days. The extract administration significant decreased food and water intake, fasting blood glucose, weight loss and polyuria, compared with untreated diabetic animals. Furthermore, oxidative stress markers particularly, lipid hydroperoxides (LOOH) and nitrite/nitrate levels, non-proteic thiol groups (RSH), γ-glutamyl-cysteine-synthetase (γ-GCS) activities and expression, heme oxygenase-1 (HO-1), endothelial and inducible nitric oxide synthases (i-NOS e-NOS) expression, significantly changed by streptozocin treatment, were markedly restored both in plasma and tissues together with nuclear sirtuins activity (Sirt1). Results suggested that <i>B. etnensis</i> bark alcoholic extract is able to counteract oxidative stress and to ameliorate some general parameters related to diabetes.https://www.mdpi.com/2076-3921/9/9/847terpenoidspolyphenolsflavonoidsbetulinic acidinsulinoxidative stress |
| spellingShingle | Giuseppe Antonio Malfa Barbara Tomasello Rosaria Acquaviva Alfonsina La Mantia Francesco Pappalardo Monica Ragusa Marcella Renis Claudia Di Giacomo The Antioxidant Activities of <i>Betula etnensis</i> Rafin. Ethanolic Extract Exert Protective and Anti-diabetic Effects on Streptozotocin-Induced Diabetes in Rats Antioxidants terpenoids polyphenols flavonoids betulinic acid insulin oxidative stress |
| title | The Antioxidant Activities of <i>Betula etnensis</i> Rafin. Ethanolic Extract Exert Protective and Anti-diabetic Effects on Streptozotocin-Induced Diabetes in Rats |
| title_full | The Antioxidant Activities of <i>Betula etnensis</i> Rafin. Ethanolic Extract Exert Protective and Anti-diabetic Effects on Streptozotocin-Induced Diabetes in Rats |
| title_fullStr | The Antioxidant Activities of <i>Betula etnensis</i> Rafin. Ethanolic Extract Exert Protective and Anti-diabetic Effects on Streptozotocin-Induced Diabetes in Rats |
| title_full_unstemmed | The Antioxidant Activities of <i>Betula etnensis</i> Rafin. Ethanolic Extract Exert Protective and Anti-diabetic Effects on Streptozotocin-Induced Diabetes in Rats |
| title_short | The Antioxidant Activities of <i>Betula etnensis</i> Rafin. Ethanolic Extract Exert Protective and Anti-diabetic Effects on Streptozotocin-Induced Diabetes in Rats |
| title_sort | antioxidant activities of i betula etnensis i rafin ethanolic extract exert protective and anti diabetic effects on streptozotocin induced diabetes in rats |
| topic | terpenoids polyphenols flavonoids betulinic acid insulin oxidative stress |
| url | https://www.mdpi.com/2076-3921/9/9/847 |
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