Appraisal and validation of a method used for detecting heavy metals in poultry feed in Bangladesh
Background and Aim: Low concentrations of heavy metals are toxic and pose a serious threat to human health and the environment. Therefore, regular assessments of the toxic metal content in poultry feed are crucial for evaluating feed quality and customer safety. It is difficult to determine the heav...
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| Format: | Article |
| Language: | English |
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Veterinary World
2022-09-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.15/September-2022/13.pdf |
| _version_ | 1828379502205468672 |
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| author | Md. Mosharaf Hossain Abu Sayeed Md. Abdul Hannan Md. Mostofa Kamal Mohammad Abul Hossain Shamshad B. Quraishi |
| author_facet | Md. Mosharaf Hossain Abu Sayeed Md. Abdul Hannan Md. Mostofa Kamal Mohammad Abul Hossain Shamshad B. Quraishi |
| author_sort | Md. Mosharaf Hossain |
| collection | DOAJ |
| description | Background and Aim: Low concentrations of heavy metals are toxic and pose a serious threat to human health and the environment. Therefore, regular assessments of the toxic metal content in poultry feed are crucial for evaluating feed quality and customer safety. It is difficult to determine the heavy metals in the poultry feed at the trace amount. Therefore, this study aimed to validate this method through the detection of three heavy metals, chromium (Cr), cadmium (Cd), and lead (Pb), in poultry feed samples.
Materials and Methods: Graphite furnace atomic absorption spectrometry (GF-AAS) method was used to analyze the heavy metals in poultry feed according to the guidelines given by the Council Directive 333/2007/EC, Commission Decision 657/2002/EC. In this study, various parameters such as linearity check, limit of detection (LOD), limit of quantification (LOQ), recovery percentage, precision checks, repeatability, reproducibility, and uncertainty measurement were considered to validate and assess the method following international guidelines. Heavy metals, such as Pb, Cr, and Cd, were analyzed from the feed samples in the laboratory using the GF-AAS method (Model: AA-7000 Shimadzu, Japan) with high purity argon as the inert gas, and the absorbance was read at wavelengths of 283.0, 357.9, and 228.8 nm, respectively.
Results: The coefficient of variation (CV%) for system suitability and precision data was <10% for all the metals (Pb, Cr, and Cd) detected in this study. The overall CV% of repeatability and reproducibility ranged from 8.70% to 8.76% and 8.65% to 9.96%, respectively. The linearity of the calibration curves was excellent (r2 > 0.999) at various concentration levels for the three different metals. The recovery (%) was found to be 94.53, 93.97, and 101.63% for Pb, Cr, and Cd, respectively. The LOD values in feed were 0.065, 0.01, and 0.11 mg/kg, and the LOQ values were 0.22, 0.03, and 0.38 mg/kg for Cr, Cd, and Pb, respectively. The values recorded for the measurement uncertainty (%) were 11.48, 4.43, and 12.42% for Cr, Cd, and Pb, respectively.
Conclusion: The results show that these study criteria or parameters have met the validated or acceptable range. Therefore, it is a reliable technique that can be used undoubtedly for the routine analysis of heavy metals in poultry feed samples across the globe. |
| first_indexed | 2024-12-10T03:41:57Z |
| format | Article |
| id | doaj.art-5db4e8abf10e4769b255c9816c9ff113 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-10T03:41:57Z |
| publishDate | 2022-09-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-5db4e8abf10e4769b255c9816c9ff1132022-12-22T02:03:32ZengVeterinary WorldVeterinary World0972-89882231-09162022-09-011592217222310.14202/vetworld.2022.2217-2223Appraisal and validation of a method used for detecting heavy metals in poultry feed in BangladeshMd. Mosharaf Hossain0Abu Sayeed Md. Abdul Hannan1Md. Mostofa Kamal2Mohammad Abul Hossain3Shamshad B. Quraishi4Department of Livestock Services, Quality Control Laboratory, Dhaka, Bangladesh.Department of Livestock Services, Quality Control Laboratory, Dhaka, Bangladesh.Department of Livestock Services, Quality Control Laboratory, Dhaka, Bangladesh.Department of Dairy and Poultry Science, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh.Analytical Chemistry Laboratory, Atomic Energy Centre, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh.Background and Aim: Low concentrations of heavy metals are toxic and pose a serious threat to human health and the environment. Therefore, regular assessments of the toxic metal content in poultry feed are crucial for evaluating feed quality and customer safety. It is difficult to determine the heavy metals in the poultry feed at the trace amount. Therefore, this study aimed to validate this method through the detection of three heavy metals, chromium (Cr), cadmium (Cd), and lead (Pb), in poultry feed samples. Materials and Methods: Graphite furnace atomic absorption spectrometry (GF-AAS) method was used to analyze the heavy metals in poultry feed according to the guidelines given by the Council Directive 333/2007/EC, Commission Decision 657/2002/EC. In this study, various parameters such as linearity check, limit of detection (LOD), limit of quantification (LOQ), recovery percentage, precision checks, repeatability, reproducibility, and uncertainty measurement were considered to validate and assess the method following international guidelines. Heavy metals, such as Pb, Cr, and Cd, were analyzed from the feed samples in the laboratory using the GF-AAS method (Model: AA-7000 Shimadzu, Japan) with high purity argon as the inert gas, and the absorbance was read at wavelengths of 283.0, 357.9, and 228.8 nm, respectively. Results: The coefficient of variation (CV%) for system suitability and precision data was <10% for all the metals (Pb, Cr, and Cd) detected in this study. The overall CV% of repeatability and reproducibility ranged from 8.70% to 8.76% and 8.65% to 9.96%, respectively. The linearity of the calibration curves was excellent (r2 > 0.999) at various concentration levels for the three different metals. The recovery (%) was found to be 94.53, 93.97, and 101.63% for Pb, Cr, and Cd, respectively. The LOD values in feed were 0.065, 0.01, and 0.11 mg/kg, and the LOQ values were 0.22, 0.03, and 0.38 mg/kg for Cr, Cd, and Pb, respectively. The values recorded for the measurement uncertainty (%) were 11.48, 4.43, and 12.42% for Cr, Cd, and Pb, respectively. Conclusion: The results show that these study criteria or parameters have met the validated or acceptable range. Therefore, it is a reliable technique that can be used undoubtedly for the routine analysis of heavy metals in poultry feed samples across the globe.http://www.veterinaryworld.org/Vol.15/September-2022/13.pdfgraphite furnace atomic absorption spectrometrymethod validationpoultry feedtoxic metals |
| spellingShingle | Md. Mosharaf Hossain Abu Sayeed Md. Abdul Hannan Md. Mostofa Kamal Mohammad Abul Hossain Shamshad B. Quraishi Appraisal and validation of a method used for detecting heavy metals in poultry feed in Bangladesh Veterinary World graphite furnace atomic absorption spectrometry method validation poultry feed toxic metals |
| title | Appraisal and validation of a method used for detecting heavy metals in poultry feed in Bangladesh |
| title_full | Appraisal and validation of a method used for detecting heavy metals in poultry feed in Bangladesh |
| title_fullStr | Appraisal and validation of a method used for detecting heavy metals in poultry feed in Bangladesh |
| title_full_unstemmed | Appraisal and validation of a method used for detecting heavy metals in poultry feed in Bangladesh |
| title_short | Appraisal and validation of a method used for detecting heavy metals in poultry feed in Bangladesh |
| title_sort | appraisal and validation of a method used for detecting heavy metals in poultry feed in bangladesh |
| topic | graphite furnace atomic absorption spectrometry method validation poultry feed toxic metals |
| url | http://www.veterinaryworld.org/Vol.15/September-2022/13.pdf |
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