Characterization of a novel thyrotropin-releasing hormone receptor, TRHR3, in chickens
The physiological roles of thyrotropin-releasing hormone (TRH) are proposed to be mediated by TRH receptors (TRHR), which have been divided into 3 subtypes, namely, TRHR1, TRHR2, and TRHR3, in vertebrates. Although 2 TRH receptors (TRHR1 and TRHR3) have been predicted to exist in birds, it remains u...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-03-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579119528267 |
| _version_ | 1819048402243026944 |
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| author | Xiaoxiao Li Zhengyang Li Yue Deng Jiannan Zhang Juan Li Yajun Wang |
| author_facet | Xiaoxiao Li Zhengyang Li Yue Deng Jiannan Zhang Juan Li Yajun Wang |
| author_sort | Xiaoxiao Li |
| collection | DOAJ |
| description | The physiological roles of thyrotropin-releasing hormone (TRH) are proposed to be mediated by TRH receptors (TRHR), which have been divided into 3 subtypes, namely, TRHR1, TRHR2, and TRHR3, in vertebrates. Although 2 TRH receptors (TRHR1 and TRHR3) have been predicted to exist in birds, it remains unclear whether TRHR3 is a functional TRH receptor similar to TRHR1. Here, we reported the functionality and tissue expression of TRHR3 in chickens. The cloned chicken TRHR3 (cTRHR3) encodes a receptor of 387 amino acids, which shares high-amino-acid identities (63–80%) to TRHR3 of parrots, lizards, Xenopus tropicalis, and tilapia and comparatively lower sequence identities to chicken TRHR1 or mouse TRHR2. Using cell-based luciferase reporter assays and Western blot, we demonstrated that similar to chicken TRHR1 (cTRHR1), cTRHR3 expressed in HEK 293 cells can be potently activated by TRH and that its activation stimulates multiple signaling pathways, indicating both TRH receptors are functional. Quantitative real-time PCR revealed that cTRHR1 and cTRHR3 are widely, but differentially, expressed in chicken tissues, and their expression is likely controlled by promoters located upstream of exon 1, which display strong promoter activities in cultured DF-1 cells. cTRHR1 is highly expressed in the anterior pituitary and testes, while cTRHR3 is highly expressed in the muscle, testes, fat, pituitary, spinal cord, and many brain regions (including hypothalamus). These findings indicate that TRH actions are likely mediated by 2 TRH receptors in chickens. In conclusion, our data provide the first piece of evidence that both cTRHR3 and cTRHR1 are functional TRH receptors, which helps to elucidate the physiological roles of TRH in birds. |
| first_indexed | 2024-12-21T11:15:41Z |
| format | Article |
| id | doaj.art-5db6d26362474d419e665538e6e8b9d8 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-21T11:15:41Z |
| publishDate | 2020-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-5db6d26362474d419e665538e6e8b9d82022-12-21T19:05:56ZengElsevierPoultry Science0032-57912020-03-0199316431654Characterization of a novel thyrotropin-releasing hormone receptor, TRHR3, in chickensXiaoxiao Li0Zhengyang Li1Yue Deng2Jiannan Zhang3Juan Li4Yajun Wang5Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, PR ChinaKey Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, PR ChinaKey Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, PR ChinaKey Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, PR ChinaCorresponding authors:; Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, PR ChinaCorresponding authors:; Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, PR ChinaThe physiological roles of thyrotropin-releasing hormone (TRH) are proposed to be mediated by TRH receptors (TRHR), which have been divided into 3 subtypes, namely, TRHR1, TRHR2, and TRHR3, in vertebrates. Although 2 TRH receptors (TRHR1 and TRHR3) have been predicted to exist in birds, it remains unclear whether TRHR3 is a functional TRH receptor similar to TRHR1. Here, we reported the functionality and tissue expression of TRHR3 in chickens. The cloned chicken TRHR3 (cTRHR3) encodes a receptor of 387 amino acids, which shares high-amino-acid identities (63–80%) to TRHR3 of parrots, lizards, Xenopus tropicalis, and tilapia and comparatively lower sequence identities to chicken TRHR1 or mouse TRHR2. Using cell-based luciferase reporter assays and Western blot, we demonstrated that similar to chicken TRHR1 (cTRHR1), cTRHR3 expressed in HEK 293 cells can be potently activated by TRH and that its activation stimulates multiple signaling pathways, indicating both TRH receptors are functional. Quantitative real-time PCR revealed that cTRHR1 and cTRHR3 are widely, but differentially, expressed in chicken tissues, and their expression is likely controlled by promoters located upstream of exon 1, which display strong promoter activities in cultured DF-1 cells. cTRHR1 is highly expressed in the anterior pituitary and testes, while cTRHR3 is highly expressed in the muscle, testes, fat, pituitary, spinal cord, and many brain regions (including hypothalamus). These findings indicate that TRH actions are likely mediated by 2 TRH receptors in chickens. In conclusion, our data provide the first piece of evidence that both cTRHR3 and cTRHR1 are functional TRH receptors, which helps to elucidate the physiological roles of TRH in birds.http://www.sciencedirect.com/science/article/pii/S0032579119528267chickenTRHTRHR3TRHR1pituitary |
| spellingShingle | Xiaoxiao Li Zhengyang Li Yue Deng Jiannan Zhang Juan Li Yajun Wang Characterization of a novel thyrotropin-releasing hormone receptor, TRHR3, in chickens Poultry Science chicken TRH TRHR3 TRHR1 pituitary |
| title | Characterization of a novel thyrotropin-releasing hormone receptor, TRHR3, in chickens |
| title_full | Characterization of a novel thyrotropin-releasing hormone receptor, TRHR3, in chickens |
| title_fullStr | Characterization of a novel thyrotropin-releasing hormone receptor, TRHR3, in chickens |
| title_full_unstemmed | Characterization of a novel thyrotropin-releasing hormone receptor, TRHR3, in chickens |
| title_short | Characterization of a novel thyrotropin-releasing hormone receptor, TRHR3, in chickens |
| title_sort | characterization of a novel thyrotropin releasing hormone receptor trhr3 in chickens |
| topic | chicken TRH TRHR3 TRHR1 pituitary |
| url | http://www.sciencedirect.com/science/article/pii/S0032579119528267 |
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