Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands
A new flow injection spectrophotometric method for the determination of <i>N</i>-acetyl-<span style="font-variant: small-caps;">l</span>-cysteine ethyl ester (NACET) was developed and validated. The method is based on the reduction of Cu(II)-ligand complexes to chro...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/22/6826 |
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| author | Lea Kukoc-Modun Tomislav Kraljević Dimitrios Tsikas Njegomir Radić Darko Modun |
| author_facet | Lea Kukoc-Modun Tomislav Kraljević Dimitrios Tsikas Njegomir Radić Darko Modun |
| author_sort | Lea Kukoc-Modun |
| collection | DOAJ |
| description | A new flow injection spectrophotometric method for the determination of <i>N</i>-acetyl-<span style="font-variant: small-caps;">l</span>-cysteine ethyl ester (NACET) was developed and validated. The method is based on the reduction of Cu(II)-ligand complexes to chromophoric Cu(I)-ligand complexes with the analyte. The studied ligands were neocuproine (NCN), bicinchoninic acid (BCA) and bathocuproine disulfonic acid (BCS). The absorbance of the Cu(I)-ligand complex was measured at 458, 562 and 483 nm for the reactions of NACET with NCN, BCA and BCS, respectively. The method was validated in terms of linear dynamic range, limit of detection and quantitation, accuracy, selectivity, and precision. Experimental conditions were optimized by a univariate method, yielding linear calibration curves in a concentration range from 2.0 × 10<sup>−6</sup> mol L<sup>−1</sup> to 2.0 × 10<sup>−4</sup> mol L<sup>−1</sup> using NCN; 2.0 × 10<sup>−6</sup> mol L<sup>−1</sup> to 1.0 × 10<sup>−4</sup> mol L<sup>−1</sup> using BCA and 6.0 × 10<sup>−7</sup> mol L<sup>−1</sup> to 1.2 × 10<sup>−4</sup> mol L<sup>−1</sup> using BCS. The achieved analytical frequency was 90 h<sup>−1</sup> for all three ligands. The method was successfully employed for NACET determination in pharmaceutical preparations, indicating that this FIA method fulfilled all the essential demands for the determination of NACET in quality control laboratories, as it combined low instrument and reagent costs with a high sampling rate. |
| first_indexed | 2024-03-10T05:14:03Z |
| format | Article |
| id | doaj.art-5db6d4ff657a4f0aa4b6d8062605c105 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:14:03Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5db6d4ff657a4f0aa4b6d8062605c1052023-11-23T00:34:20ZengMDPI AGMolecules1420-30492021-11-012622682610.3390/molecules26226826Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive LigandsLea Kukoc-Modun0Tomislav Kraljević1Dimitrios Tsikas2Njegomir Radić3Darko Modun4Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, CroatiaDepartment of Chemistry, Faculty of Science and Education, University of Mostar, Matice hrvatske bb, 88000 Mostar, Bosnia and HerzegovinaInstitute of Toxicology, Core Unit Proteomics, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, GermanyDepartment of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, CroatiaDepartment of Pharmacy, School of Medicine, University of Split, Šoltanska 2, 21000 Split, CroatiaA new flow injection spectrophotometric method for the determination of <i>N</i>-acetyl-<span style="font-variant: small-caps;">l</span>-cysteine ethyl ester (NACET) was developed and validated. The method is based on the reduction of Cu(II)-ligand complexes to chromophoric Cu(I)-ligand complexes with the analyte. The studied ligands were neocuproine (NCN), bicinchoninic acid (BCA) and bathocuproine disulfonic acid (BCS). The absorbance of the Cu(I)-ligand complex was measured at 458, 562 and 483 nm for the reactions of NACET with NCN, BCA and BCS, respectively. The method was validated in terms of linear dynamic range, limit of detection and quantitation, accuracy, selectivity, and precision. Experimental conditions were optimized by a univariate method, yielding linear calibration curves in a concentration range from 2.0 × 10<sup>−6</sup> mol L<sup>−1</sup> to 2.0 × 10<sup>−4</sup> mol L<sup>−1</sup> using NCN; 2.0 × 10<sup>−6</sup> mol L<sup>−1</sup> to 1.0 × 10<sup>−4</sup> mol L<sup>−1</sup> using BCA and 6.0 × 10<sup>−7</sup> mol L<sup>−1</sup> to 1.2 × 10<sup>−4</sup> mol L<sup>−1</sup> using BCS. The achieved analytical frequency was 90 h<sup>−1</sup> for all three ligands. The method was successfully employed for NACET determination in pharmaceutical preparations, indicating that this FIA method fulfilled all the essential demands for the determination of NACET in quality control laboratories, as it combined low instrument and reagent costs with a high sampling rate.https://www.mdpi.com/1420-3049/26/22/6826<i>N</i>-acetyl-<span style="font-variant: small-caps">l</span>-cysteine ethyl esterneocuproinebicinchoninic acidbathocuproine disulfonic acidflow injection analysisspectrophotometric determination |
| spellingShingle | Lea Kukoc-Modun Tomislav Kraljević Dimitrios Tsikas Njegomir Radić Darko Modun Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands Molecules <i>N</i>-acetyl-<span style="font-variant: small-caps">l</span>-cysteine ethyl ester neocuproine bicinchoninic acid bathocuproine disulfonic acid flow injection analysis spectrophotometric determination |
| title | Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands |
| title_full | Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands |
| title_fullStr | Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands |
| title_full_unstemmed | Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands |
| title_short | Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands |
| title_sort | determination of i n i acetyl span style font variant small caps l span cysteine ethyl ester nacet by flow injection analysis and spectrophotometric detection using different thiol sensitive ligands |
| topic | <i>N</i>-acetyl-<span style="font-variant: small-caps">l</span>-cysteine ethyl ester neocuproine bicinchoninic acid bathocuproine disulfonic acid flow injection analysis spectrophotometric determination |
| url | https://www.mdpi.com/1420-3049/26/22/6826 |
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