Synthesis of Adenine-based Fluorescent and Naked-eye Chemosensors: Specific DNA Probes for The Detection of Bacterial Pathogens
A rapid and confident tool to identify and diagnose bacterial pathogens with more accuracy using DNA as fingerprints is necessary. Herein, we report a smart chemosensor having a terminal adenine sticking to the thymine of single-stranded DNA (ssDNA) through supramolecular interactions and, which lea...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Slovenian Chemical Society
2021-12-01
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| Series: | Acta Chimica Slovenica |
| Subjects: | |
| Online Access: | https://journals.matheo.si/index.php/ACSi/article/view/6749 |
| _version_ | 1819096713070116864 |
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| author | Burak Şener Ömür Baysal Said Nadeem Ragıp Soner Silme |
| author_facet | Burak Şener Ömür Baysal Said Nadeem Ragıp Soner Silme |
| author_sort | Burak Şener |
| collection | DOAJ |
| description | A rapid and confident tool to identify and diagnose bacterial pathogens with more accuracy using DNA as fingerprints is necessary. Herein, we report a smart chemosensor having a terminal adenine sticking to the thymine of single-stranded DNA (ssDNA) through supramolecular interactions and, which leaves ssDNA when the same ssDNA matches with the targeting desired DNA. We have synthesized a naked-eye coloured chemosensor with carbazole. As a model genetic material, DNA of Clavibacter michiganensis subsp. michiganensis was hybridized to ssDNA and immobilized over nitrocellulose membrane. The prepared adenine-chemosensor, by passing through the nitrocellulose-ssDNA membrane caused the formation of ssDNA nitrocellulose-ssDNA-adenine-chemosensor. FTIR results of the immobilized ssDNAs showed that the matching of same ssDNA releases the adenine-chemosensor from the surface of nitrocellulose-ssDNA that results in formation of the double stranded DNA. The selectivity of chemosensor was also confirmed with different bacterial DNA (Bacillus subtilis) as control. These data highlights accurate and reliable results of a new diagnostic kit prototype promising for further studies, which is able to diagnose DNA quickly and precisely. |
| first_indexed | 2024-12-22T00:03:34Z |
| format | Article |
| id | doaj.art-5db921fe9a534aaeaf711856d94b00d1 |
| institution | Directory Open Access Journal |
| issn | 1318-0207 1580-3155 |
| language | English |
| last_indexed | 2024-12-22T00:03:34Z |
| publishDate | 2021-12-01 |
| publisher | Slovenian Chemical Society |
| record_format | Article |
| series | Acta Chimica Slovenica |
| spelling | doaj.art-5db921fe9a534aaeaf711856d94b00d12022-12-21T18:45:37ZengSlovenian Chemical SocietyActa Chimica Slovenica1318-02071580-31552021-12-0168478179010.17344/acsi.2021.67491003Synthesis of Adenine-based Fluorescent and Naked-eye Chemosensors: Specific DNA Probes for The Detection of Bacterial PathogensBurak Şener0Ömür Baysal1Said Nadeem2Ragıp Soner Silme3Muğla Sıtkı Koçman UniversityMuğla Sıtkı Koçman UniversityAydın Adnan Menderes UniversityIstanbul UniversityA rapid and confident tool to identify and diagnose bacterial pathogens with more accuracy using DNA as fingerprints is necessary. Herein, we report a smart chemosensor having a terminal adenine sticking to the thymine of single-stranded DNA (ssDNA) through supramolecular interactions and, which leaves ssDNA when the same ssDNA matches with the targeting desired DNA. We have synthesized a naked-eye coloured chemosensor with carbazole. As a model genetic material, DNA of Clavibacter michiganensis subsp. michiganensis was hybridized to ssDNA and immobilized over nitrocellulose membrane. The prepared adenine-chemosensor, by passing through the nitrocellulose-ssDNA membrane caused the formation of ssDNA nitrocellulose-ssDNA-adenine-chemosensor. FTIR results of the immobilized ssDNAs showed that the matching of same ssDNA releases the adenine-chemosensor from the surface of nitrocellulose-ssDNA that results in formation of the double stranded DNA. The selectivity of chemosensor was also confirmed with different bacterial DNA (Bacillus subtilis) as control. These data highlights accurate and reliable results of a new diagnostic kit prototype promising for further studies, which is able to diagnose DNA quickly and precisely.https://journals.matheo.si/index.php/ACSi/article/view/6749naked-eye chemosensordna probekit designingdetectionbacterial pathogensnanotechnology |
| spellingShingle | Burak Şener Ömür Baysal Said Nadeem Ragıp Soner Silme Synthesis of Adenine-based Fluorescent and Naked-eye Chemosensors: Specific DNA Probes for The Detection of Bacterial Pathogens Acta Chimica Slovenica naked-eye chemosensor dna probe kit designing detection bacterial pathogens nanotechnology |
| title | Synthesis of Adenine-based Fluorescent and Naked-eye Chemosensors: Specific DNA Probes for The Detection of Bacterial Pathogens |
| title_full | Synthesis of Adenine-based Fluorescent and Naked-eye Chemosensors: Specific DNA Probes for The Detection of Bacterial Pathogens |
| title_fullStr | Synthesis of Adenine-based Fluorescent and Naked-eye Chemosensors: Specific DNA Probes for The Detection of Bacterial Pathogens |
| title_full_unstemmed | Synthesis of Adenine-based Fluorescent and Naked-eye Chemosensors: Specific DNA Probes for The Detection of Bacterial Pathogens |
| title_short | Synthesis of Adenine-based Fluorescent and Naked-eye Chemosensors: Specific DNA Probes for The Detection of Bacterial Pathogens |
| title_sort | synthesis of adenine based fluorescent and naked eye chemosensors specific dna probes for the detection of bacterial pathogens |
| topic | naked-eye chemosensor dna probe kit designing detection bacterial pathogens nanotechnology |
| url | https://journals.matheo.si/index.php/ACSi/article/view/6749 |
| work_keys_str_mv | AT buraksener synthesisofadeninebasedfluorescentandnakedeyechemosensorsspecificdnaprobesforthedetectionofbacterialpathogens AT omurbaysal synthesisofadeninebasedfluorescentandnakedeyechemosensorsspecificdnaprobesforthedetectionofbacterialpathogens AT saidnadeem synthesisofadeninebasedfluorescentandnakedeyechemosensorsspecificdnaprobesforthedetectionofbacterialpathogens AT ragıpsonersilme synthesisofadeninebasedfluorescentandnakedeyechemosensorsspecificdnaprobesforthedetectionofbacterialpathogens |