Effects of a skin-massaging device on the ex-vivo expression of human dermis proteins and in-vivo facial wrinkles.
Mechanical and geometrical cues influence cell behaviour. At the tissue level, almost all organs exhibit immediate mechanical responsiveness, in particular by increasing their stiffness in direct proportion to an applied mechanical stress. It was recently shown in cultured-cell models, in particular...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5383004?pdf=render |
| _version_ | 1811199754977148928 |
|---|---|
| author | Elisa Caberlotto Laetitia Ruiz Zane Miller Mickael Poletti Lauri Tadlock |
| author_facet | Elisa Caberlotto Laetitia Ruiz Zane Miller Mickael Poletti Lauri Tadlock |
| author_sort | Elisa Caberlotto |
| collection | DOAJ |
| description | Mechanical and geometrical cues influence cell behaviour. At the tissue level, almost all organs exhibit immediate mechanical responsiveness, in particular by increasing their stiffness in direct proportion to an applied mechanical stress. It was recently shown in cultured-cell models, in particular with fibroblasts, that the frequency of the applied stress is a fundamental stimulating parameter. However, the influence of the stimulus frequency at the tissue level has remained elusive. Using a device to deliver an oscillating torque that generates cyclic strain at different frequencies, we studied the effect(s) of mild skin massage in an ex vivo model and in vivo. Skin explants were maintained ex vivo for 10 days and massaged twice daily for one minute at various frequencies within the range of 65-85 Hz. Biopsies were analysed at D0, D5 and D10 and processed for immuno-histological staining specific to various dermal proteins. As compared to untreated skin explants, the massaging procedure clearly led to higher rates of expression, in particular for decorin, fibrillin, tropoelastin, and procollagen-1. The mechanical stimulus thus evoked an anti-aging response. Strikingly, the expression was found to depend on the stimulus frequency with maximum expression at 75Hz. We then tested whether this mechanical stimulus had an anti-aging effect in vivo. Twenty Caucasian women (aged 65-75y) applied a commercial anti-aging cream to the face and neck, followed by daily treatments using the anti-aging massage device for 8 weeks. A control group of twenty-two women, with similar ages to the first group, applied the cream alone. At W0, W4 and W8, a blinded evaluator assessed the global facial wrinkles, skin texture, lip area, cheek wrinkles, neck sagging and neck texture using a clinical grading scale. We found that combining the massaging device with a skin anti-aging formulation amplified the beneficial effects of the cream. |
| first_indexed | 2024-04-12T01:53:42Z |
| format | Article |
| id | doaj.art-5dba04a546c247198aec1389c27a2247 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T01:53:42Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5dba04a546c247198aec1389c27a22472022-12-22T03:52:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017262410.1371/journal.pone.0172624Effects of a skin-massaging device on the ex-vivo expression of human dermis proteins and in-vivo facial wrinkles.Elisa CaberlottoLaetitia RuizZane MillerMickael PolettiLauri TadlockMechanical and geometrical cues influence cell behaviour. At the tissue level, almost all organs exhibit immediate mechanical responsiveness, in particular by increasing their stiffness in direct proportion to an applied mechanical stress. It was recently shown in cultured-cell models, in particular with fibroblasts, that the frequency of the applied stress is a fundamental stimulating parameter. However, the influence of the stimulus frequency at the tissue level has remained elusive. Using a device to deliver an oscillating torque that generates cyclic strain at different frequencies, we studied the effect(s) of mild skin massage in an ex vivo model and in vivo. Skin explants were maintained ex vivo for 10 days and massaged twice daily for one minute at various frequencies within the range of 65-85 Hz. Biopsies were analysed at D0, D5 and D10 and processed for immuno-histological staining specific to various dermal proteins. As compared to untreated skin explants, the massaging procedure clearly led to higher rates of expression, in particular for decorin, fibrillin, tropoelastin, and procollagen-1. The mechanical stimulus thus evoked an anti-aging response. Strikingly, the expression was found to depend on the stimulus frequency with maximum expression at 75Hz. We then tested whether this mechanical stimulus had an anti-aging effect in vivo. Twenty Caucasian women (aged 65-75y) applied a commercial anti-aging cream to the face and neck, followed by daily treatments using the anti-aging massage device for 8 weeks. A control group of twenty-two women, with similar ages to the first group, applied the cream alone. At W0, W4 and W8, a blinded evaluator assessed the global facial wrinkles, skin texture, lip area, cheek wrinkles, neck sagging and neck texture using a clinical grading scale. We found that combining the massaging device with a skin anti-aging formulation amplified the beneficial effects of the cream.http://europepmc.org/articles/PMC5383004?pdf=render |
| spellingShingle | Elisa Caberlotto Laetitia Ruiz Zane Miller Mickael Poletti Lauri Tadlock Effects of a skin-massaging device on the ex-vivo expression of human dermis proteins and in-vivo facial wrinkles. PLoS ONE |
| title | Effects of a skin-massaging device on the ex-vivo expression of human dermis proteins and in-vivo facial wrinkles. |
| title_full | Effects of a skin-massaging device on the ex-vivo expression of human dermis proteins and in-vivo facial wrinkles. |
| title_fullStr | Effects of a skin-massaging device on the ex-vivo expression of human dermis proteins and in-vivo facial wrinkles. |
| title_full_unstemmed | Effects of a skin-massaging device on the ex-vivo expression of human dermis proteins and in-vivo facial wrinkles. |
| title_short | Effects of a skin-massaging device on the ex-vivo expression of human dermis proteins and in-vivo facial wrinkles. |
| title_sort | effects of a skin massaging device on the ex vivo expression of human dermis proteins and in vivo facial wrinkles |
| url | http://europepmc.org/articles/PMC5383004?pdf=render |
| work_keys_str_mv | AT elisacaberlotto effectsofaskinmassagingdeviceontheexvivoexpressionofhumandermisproteinsandinvivofacialwrinkles AT laetitiaruiz effectsofaskinmassagingdeviceontheexvivoexpressionofhumandermisproteinsandinvivofacialwrinkles AT zanemiller effectsofaskinmassagingdeviceontheexvivoexpressionofhumandermisproteinsandinvivofacialwrinkles AT mickaelpoletti effectsofaskinmassagingdeviceontheexvivoexpressionofhumandermisproteinsandinvivofacialwrinkles AT lauritadlock effectsofaskinmassagingdeviceontheexvivoexpressionofhumandermisproteinsandinvivofacialwrinkles |