Validation and comparison of reference genes for qPCR normalization of celery (Apium graveolens) at different development stages
A suitable reference gene is an important prerequisite for guarantying accurate and reliable results in qPCR analysis. Celery is one of the representative vegetable in Apiaceae and is widely cultivated and consumed in the world. However, no reports have been previously published concerning reference...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-03-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00313/full |
| _version_ | 1818887182546370560 |
|---|---|
| author | Meng-Yao eLi Feng eWang Qian eJiang Guang-Long eWang Chang eTian Ai-Sheng eXiong |
| author_facet | Meng-Yao eLi Feng eWang Qian eJiang Guang-Long eWang Chang eTian Ai-Sheng eXiong |
| author_sort | Meng-Yao eLi |
| collection | DOAJ |
| description | A suitable reference gene is an important prerequisite for guarantying accurate and reliable results in qPCR analysis. Celery is one of the representative vegetable in Apiaceae and is widely cultivated and consumed in the world. However, no reports have been previously published concerning reference genes in celery. In this study, the expression stabilities of nine candidate reference genes in leaf blade and petiole at different development stages were evaluated using three statistics algorithms geNorm, NormFinder, and BestKeeper. Our results showed that TUB-B, TUB-A, and UBC were the most reference genes among all tested samples. GAPDH represented the maximum stability for most individual sample, while the UBQ displayed the minimum stability. To further validate the stability of reference genes, the expression pattern of AgAP2-2 was calculated by using the selected genes for normalization. In addition, the expression patterns of several development-related genes were studied using the selected reference gene. Our results will be beneficial for further studies on gene transcription in celery. |
| first_indexed | 2024-12-19T16:33:10Z |
| format | Article |
| id | doaj.art-5dbe4d129e074d09b5560f29f42c2686 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T16:33:10Z |
| publishDate | 2016-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5dbe4d129e074d09b5560f29f42c26862022-12-21T20:14:05ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-03-01710.3389/fpls.2016.00313178149Validation and comparison of reference genes for qPCR normalization of celery (Apium graveolens) at different development stagesMeng-Yao eLi0Feng eWang1Qian eJiang2Guang-Long eWang3Chang eTian4Ai-Sheng eXiong5Nanjing Agricultural UniversityNanjing Agricultural UniversityNanjing Agricultural UniversityNanjing Agricultural UniversityNanjing Agricultural UniversityNanjing Agricultural UniversityA suitable reference gene is an important prerequisite for guarantying accurate and reliable results in qPCR analysis. Celery is one of the representative vegetable in Apiaceae and is widely cultivated and consumed in the world. However, no reports have been previously published concerning reference genes in celery. In this study, the expression stabilities of nine candidate reference genes in leaf blade and petiole at different development stages were evaluated using three statistics algorithms geNorm, NormFinder, and BestKeeper. Our results showed that TUB-B, TUB-A, and UBC were the most reference genes among all tested samples. GAPDH represented the maximum stability for most individual sample, while the UBQ displayed the minimum stability. To further validate the stability of reference genes, the expression pattern of AgAP2-2 was calculated by using the selected genes for normalization. In addition, the expression patterns of several development-related genes were studied using the selected reference gene. Our results will be beneficial for further studies on gene transcription in celery.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00313/fullGene ExpressionqPCRreference geneDevelopmental Stagecelery |
| spellingShingle | Meng-Yao eLi Feng eWang Qian eJiang Guang-Long eWang Chang eTian Ai-Sheng eXiong Validation and comparison of reference genes for qPCR normalization of celery (Apium graveolens) at different development stages Frontiers in Plant Science Gene Expression qPCR reference gene Developmental Stage celery |
| title | Validation and comparison of reference genes for qPCR normalization of celery (Apium graveolens) at different development stages |
| title_full | Validation and comparison of reference genes for qPCR normalization of celery (Apium graveolens) at different development stages |
| title_fullStr | Validation and comparison of reference genes for qPCR normalization of celery (Apium graveolens) at different development stages |
| title_full_unstemmed | Validation and comparison of reference genes for qPCR normalization of celery (Apium graveolens) at different development stages |
| title_short | Validation and comparison of reference genes for qPCR normalization of celery (Apium graveolens) at different development stages |
| title_sort | validation and comparison of reference genes for qpcr normalization of celery apium graveolens at different development stages |
| topic | Gene Expression qPCR reference gene Developmental Stage celery |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00313/full |
| work_keys_str_mv | AT mengyaoeli validationandcomparisonofreferencegenesforqpcrnormalizationofceleryapiumgraveolensatdifferentdevelopmentstages AT fengewang validationandcomparisonofreferencegenesforqpcrnormalizationofceleryapiumgraveolensatdifferentdevelopmentstages AT qianejiang validationandcomparisonofreferencegenesforqpcrnormalizationofceleryapiumgraveolensatdifferentdevelopmentstages AT guanglongewang validationandcomparisonofreferencegenesforqpcrnormalizationofceleryapiumgraveolensatdifferentdevelopmentstages AT changetian validationandcomparisonofreferencegenesforqpcrnormalizationofceleryapiumgraveolensatdifferentdevelopmentstages AT aishengexiong validationandcomparisonofreferencegenesforqpcrnormalizationofceleryapiumgraveolensatdifferentdevelopmentstages |