Quercetin as an Auxiliary Endodontic Irrigant for Root Canal Treatment: Anti-Biofilm and Dentin Collagen-Stabilizing Effects In Vitro
Bacterial reinfection and root fracture are the main culprits related to root canal treatment failure. This study aimed to assess the utility of quercetin solution as an adjunctive endodontic irrigant that does not weaken root canal dentin with commitment anti-biofilm activity and bio-safety. Based...
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2021-03-01
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| author | Zhuo Liu Xiangli Feng Xiangyao Wang Shiyuan Yang Jing Mao Shiqiang Gong |
| author_facet | Zhuo Liu Xiangli Feng Xiangyao Wang Shiyuan Yang Jing Mao Shiqiang Gong |
| author_sort | Zhuo Liu |
| collection | DOAJ |
| description | Bacterial reinfection and root fracture are the main culprits related to root canal treatment failure. This study aimed to assess the utility of quercetin solution as an adjunctive endodontic irrigant that does not weaken root canal dentin with commitment anti-biofilm activity and bio-safety. Based on a noninvasive dentin infection model, dentin tubules infected with <i>Enterococcus faecalis</i> (<i>E. faecalis</i>) were irrigated with sterile water (control group), and 0, 1, 2, 4 wt% quercetin-containing ethanol solutions. Live and dead bacteria percentages in <i>E. faecalis</i> biofilms were analyzed by confocal laser scanning microscopy (CLSM). Elastic modulus, hydroxyproline release and X-ray photoelectron spectroscopy (XPS) characterization were tested to evaluate the irrigants’ collagen-stabilizing effect. The cytotoxicity was tested by CCK-8 assay. Quercetin increased the proportion of dead bacteria volumes within <i>E. faecalis</i> and improved the flexural strength of dentin compared to control group (<i>p</i> < 0.05). Quercetin-treated dentin matrix had less elasticity loss and hydroxyproline release after collagenase degradation (<i>p</i> < 0.05). Moreover, quercetin solutions revealed an increase in the C-O peak area under both C1s and O1s narrow-scan spectra of XPS characterization, and no cytotoxicity (<i>p</i> > 0.05). Quercetin exhibited anti-biofilm activity, a collagen-stabilizing effect with cytocompatibility, supporting quercetin as a potential candidate for endodontic irrigant. |
| first_indexed | 2024-03-09T05:52:10Z |
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| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T05:52:10Z |
| publishDate | 2021-03-01 |
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| spelling | doaj.art-5dc0b69335154b16a4492ef976bd1e182023-12-03T12:16:25ZengMDPI AGMaterials1996-19442021-03-01145117810.3390/ma14051178Quercetin as an Auxiliary Endodontic Irrigant for Root Canal Treatment: Anti-Biofilm and Dentin Collagen-Stabilizing Effects In VitroZhuo Liu0Xiangli Feng1Xiangyao Wang2Shiyuan Yang3Jing Mao4Shiqiang Gong5Center of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Stomatology, Hubei Provincial Hospital of TCM, Wuhan 430061, ChinaCenter of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaCenter of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaCenter of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaCenter of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaBacterial reinfection and root fracture are the main culprits related to root canal treatment failure. This study aimed to assess the utility of quercetin solution as an adjunctive endodontic irrigant that does not weaken root canal dentin with commitment anti-biofilm activity and bio-safety. Based on a noninvasive dentin infection model, dentin tubules infected with <i>Enterococcus faecalis</i> (<i>E. faecalis</i>) were irrigated with sterile water (control group), and 0, 1, 2, 4 wt% quercetin-containing ethanol solutions. Live and dead bacteria percentages in <i>E. faecalis</i> biofilms were analyzed by confocal laser scanning microscopy (CLSM). Elastic modulus, hydroxyproline release and X-ray photoelectron spectroscopy (XPS) characterization were tested to evaluate the irrigants’ collagen-stabilizing effect. The cytotoxicity was tested by CCK-8 assay. Quercetin increased the proportion of dead bacteria volumes within <i>E. faecalis</i> and improved the flexural strength of dentin compared to control group (<i>p</i> < 0.05). Quercetin-treated dentin matrix had less elasticity loss and hydroxyproline release after collagenase degradation (<i>p</i> < 0.05). Moreover, quercetin solutions revealed an increase in the C-O peak area under both C1s and O1s narrow-scan spectra of XPS characterization, and no cytotoxicity (<i>p</i> > 0.05). Quercetin exhibited anti-biofilm activity, a collagen-stabilizing effect with cytocompatibility, supporting quercetin as a potential candidate for endodontic irrigant.https://www.mdpi.com/1996-1944/14/5/1178antibacterial activityendodontic irrigantenterococcus faecalisquercetinroot canal |
| spellingShingle | Zhuo Liu Xiangli Feng Xiangyao Wang Shiyuan Yang Jing Mao Shiqiang Gong Quercetin as an Auxiliary Endodontic Irrigant for Root Canal Treatment: Anti-Biofilm and Dentin Collagen-Stabilizing Effects In Vitro Materials antibacterial activity endodontic irrigant enterococcus faecalis quercetin root canal |
| title | Quercetin as an Auxiliary Endodontic Irrigant for Root Canal Treatment: Anti-Biofilm and Dentin Collagen-Stabilizing Effects In Vitro |
| title_full | Quercetin as an Auxiliary Endodontic Irrigant for Root Canal Treatment: Anti-Biofilm and Dentin Collagen-Stabilizing Effects In Vitro |
| title_fullStr | Quercetin as an Auxiliary Endodontic Irrigant for Root Canal Treatment: Anti-Biofilm and Dentin Collagen-Stabilizing Effects In Vitro |
| title_full_unstemmed | Quercetin as an Auxiliary Endodontic Irrigant for Root Canal Treatment: Anti-Biofilm and Dentin Collagen-Stabilizing Effects In Vitro |
| title_short | Quercetin as an Auxiliary Endodontic Irrigant for Root Canal Treatment: Anti-Biofilm and Dentin Collagen-Stabilizing Effects In Vitro |
| title_sort | quercetin as an auxiliary endodontic irrigant for root canal treatment anti biofilm and dentin collagen stabilizing effects in vitro |
| topic | antibacterial activity endodontic irrigant enterococcus faecalis quercetin root canal |
| url | https://www.mdpi.com/1996-1944/14/5/1178 |
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