Characterization of a Vibriophage Infecting Pathogenic <i>Vibrio harveyi</i>
Bacterial diseases caused by <i>Vibrio</i> spp. are prevalent in aquaculture and can lead to high mortality rates among aquatic species and significant economic losses. With the increasing emergence of multidrug-resistant <i>Vibrio</i> strains, phage therapy is being explored...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/22/16202 |
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| author | Yingying Li Huayi Yun Ruo Chen Nianzhi Jiao Qiang Zheng Yunlan Yang Rui Zhang |
| author_facet | Yingying Li Huayi Yun Ruo Chen Nianzhi Jiao Qiang Zheng Yunlan Yang Rui Zhang |
| author_sort | Yingying Li |
| collection | DOAJ |
| description | Bacterial diseases caused by <i>Vibrio</i> spp. are prevalent in aquaculture and can lead to high mortality rates among aquatic species and significant economic losses. With the increasing emergence of multidrug-resistant <i>Vibrio</i> strains, phage therapy is being explored as a potential alternative to antibiotics for biocontrol of infectious diseases. Here, a new lytic phage named vB_VhaS_R21Y (R21Y) was isolated against <i>Vibrio harveyi</i> BVH1 obtained from seawater from a scallop-farming area in Rongcheng, China. Its morphology, infection cycle, lytic profile, phage stability, and genetic features were characterized. Transmission electronic microscopy indicated that R21Y is siphovirus-like, comprising an icosahedral head (diameter 73.31 ± 2.09 nm) and long noncontractile tail (205.55 ± 0.75 nm). In a one-step growth experiment, R21Y had a 40-min latent period and a burst size of 35 phage particles per infected cell. R21Y was highly species-specific in the host range test and was relatively stable at pH 4–10 and 4–55 °C. Genomic analysis showed that R21Y is a double-stranded DNA virus with a genome size of 82,795 bp and GC content of 47.48%. Its high tolerance and lytic activity indicated that R21Y may be a candidate for phage therapy in controlling vibriosis in aquacultural systems. |
| first_indexed | 2024-03-09T16:45:53Z |
| format | Article |
| id | doaj.art-5dc0c1deac88427f8f275444c228cb4e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:45:53Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5dc0c1deac88427f8f275444c228cb4e2023-11-24T14:46:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221620210.3390/ijms242216202Characterization of a Vibriophage Infecting Pathogenic <i>Vibrio harveyi</i>Yingying Li0Huayi Yun1Ruo Chen2Nianzhi Jiao3Qiang Zheng4Yunlan Yang5Rui Zhang6State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, ChinaInstitute for Advanced Study, Shenzhen University, Shenzhen 518061, ChinaInstitute for Advanced Study, Shenzhen University, Shenzhen 518061, ChinaBacterial diseases caused by <i>Vibrio</i> spp. are prevalent in aquaculture and can lead to high mortality rates among aquatic species and significant economic losses. With the increasing emergence of multidrug-resistant <i>Vibrio</i> strains, phage therapy is being explored as a potential alternative to antibiotics for biocontrol of infectious diseases. Here, a new lytic phage named vB_VhaS_R21Y (R21Y) was isolated against <i>Vibrio harveyi</i> BVH1 obtained from seawater from a scallop-farming area in Rongcheng, China. Its morphology, infection cycle, lytic profile, phage stability, and genetic features were characterized. Transmission electronic microscopy indicated that R21Y is siphovirus-like, comprising an icosahedral head (diameter 73.31 ± 2.09 nm) and long noncontractile tail (205.55 ± 0.75 nm). In a one-step growth experiment, R21Y had a 40-min latent period and a burst size of 35 phage particles per infected cell. R21Y was highly species-specific in the host range test and was relatively stable at pH 4–10 and 4–55 °C. Genomic analysis showed that R21Y is a double-stranded DNA virus with a genome size of 82,795 bp and GC content of 47.48%. Its high tolerance and lytic activity indicated that R21Y may be a candidate for phage therapy in controlling vibriosis in aquacultural systems.https://www.mdpi.com/1422-0067/24/22/16202<i>Vibrio</i> phagesiphovirusbiological characteristicsgenomic analysisphage therapy |
| spellingShingle | Yingying Li Huayi Yun Ruo Chen Nianzhi Jiao Qiang Zheng Yunlan Yang Rui Zhang Characterization of a Vibriophage Infecting Pathogenic <i>Vibrio harveyi</i> International Journal of Molecular Sciences <i>Vibrio</i> phage siphovirus biological characteristics genomic analysis phage therapy |
| title | Characterization of a Vibriophage Infecting Pathogenic <i>Vibrio harveyi</i> |
| title_full | Characterization of a Vibriophage Infecting Pathogenic <i>Vibrio harveyi</i> |
| title_fullStr | Characterization of a Vibriophage Infecting Pathogenic <i>Vibrio harveyi</i> |
| title_full_unstemmed | Characterization of a Vibriophage Infecting Pathogenic <i>Vibrio harveyi</i> |
| title_short | Characterization of a Vibriophage Infecting Pathogenic <i>Vibrio harveyi</i> |
| title_sort | characterization of a vibriophage infecting pathogenic i vibrio harveyi i |
| topic | <i>Vibrio</i> phage siphovirus biological characteristics genomic analysis phage therapy |
| url | https://www.mdpi.com/1422-0067/24/22/16202 |
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