The identification of protein and RNA interactors of the splicing factor Caper in the adult Drosophila nervous system
Post-transcriptional gene regulation is a fundamental mechanism that helps regulate the development and healthy aging of the nervous system. Mutations that disrupt the function of RNA-binding proteins (RBPs), which regulate post-transcriptional gene regulation, have increasingly been implicated in n...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-06-01
|
| Series: | Frontiers in Molecular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2023.1114857/full |
| _version_ | 1827916076932923392 |
|---|---|
| author | M. Brandon Titus Adeline W. Chang Niko Popitsch Christopher C. Ebmeier Jeremy M. Bono Eugenia C. Olesnicky |
| author_facet | M. Brandon Titus Adeline W. Chang Niko Popitsch Christopher C. Ebmeier Jeremy M. Bono Eugenia C. Olesnicky |
| author_sort | M. Brandon Titus |
| collection | DOAJ |
| description | Post-transcriptional gene regulation is a fundamental mechanism that helps regulate the development and healthy aging of the nervous system. Mutations that disrupt the function of RNA-binding proteins (RBPs), which regulate post-transcriptional gene regulation, have increasingly been implicated in neurological disorders including amyotrophic lateral sclerosis, Fragile X Syndrome, and spinal muscular atrophy. Interestingly, although the majority of RBPs are expressed widely within diverse tissue types, the nervous system is often particularly sensitive to their dysfunction. It is therefore critical to elucidate how aberrant RNA regulation that results from the dysfunction of ubiquitously expressed RBPs leads to tissue specific pathologies that underlie neurological diseases. The highly conserved RBP and alternative splicing factor Caper is widely expressed throughout development and is required for the development of Drosophila sensory and motor neurons. Furthermore, caper dysfunction results in larval and adult locomotor deficits. Nonetheless, little is known about which proteins interact with Caper, and which RNAs are regulated by Caper. Here we identify proteins that interact with Caper in both neural and muscle tissue, along with neural specific Caper target RNAs. Furthermore, we show that a subset of these Caper-interacting proteins and RNAs genetically interact with caper to regulate Drosophila gravitaxis behavior. |
| first_indexed | 2024-03-13T03:08:47Z |
| format | Article |
| id | doaj.art-5dd07272bdad45079db99203363d36f7 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-03-13T03:08:47Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-5dd07272bdad45079db99203363d36f72023-06-26T15:59:08ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992023-06-011610.3389/fnmol.2023.11148571114857The identification of protein and RNA interactors of the splicing factor Caper in the adult Drosophila nervous systemM. Brandon Titus0Adeline W. Chang1Niko Popitsch2Christopher C. Ebmeier3Jeremy M. Bono4Eugenia C. Olesnicky5Department of Biology, University of Colorado Colorado Springs, Colorado Springs, CO, United StatesDepartment of Biology, University of Colorado Colorado Springs, Colorado Springs, CO, United StatesDepartment of Biochemistry and Cell Biology, Max Perutz Labs, University of Vienna, Vienna, AustriaDepartment of Biochemistry, University of Colorado Boulder, Boulder, CO, United StatesDepartment of Biology, University of Colorado Colorado Springs, Colorado Springs, CO, United StatesDepartment of Biology, University of Colorado Colorado Springs, Colorado Springs, CO, United StatesPost-transcriptional gene regulation is a fundamental mechanism that helps regulate the development and healthy aging of the nervous system. Mutations that disrupt the function of RNA-binding proteins (RBPs), which regulate post-transcriptional gene regulation, have increasingly been implicated in neurological disorders including amyotrophic lateral sclerosis, Fragile X Syndrome, and spinal muscular atrophy. Interestingly, although the majority of RBPs are expressed widely within diverse tissue types, the nervous system is often particularly sensitive to their dysfunction. It is therefore critical to elucidate how aberrant RNA regulation that results from the dysfunction of ubiquitously expressed RBPs leads to tissue specific pathologies that underlie neurological diseases. The highly conserved RBP and alternative splicing factor Caper is widely expressed throughout development and is required for the development of Drosophila sensory and motor neurons. Furthermore, caper dysfunction results in larval and adult locomotor deficits. Nonetheless, little is known about which proteins interact with Caper, and which RNAs are regulated by Caper. Here we identify proteins that interact with Caper in both neural and muscle tissue, along with neural specific Caper target RNAs. Furthermore, we show that a subset of these Caper-interacting proteins and RNAs genetically interact with caper to regulate Drosophila gravitaxis behavior.https://www.frontiersin.org/articles/10.3389/fnmol.2023.1114857/fullalternative splicingRNA-binding proteinscaperRBM39Drosophilatissue specific function |
| spellingShingle | M. Brandon Titus Adeline W. Chang Niko Popitsch Christopher C. Ebmeier Jeremy M. Bono Eugenia C. Olesnicky The identification of protein and RNA interactors of the splicing factor Caper in the adult Drosophila nervous system Frontiers in Molecular Neuroscience alternative splicing RNA-binding proteins caper RBM39 Drosophila tissue specific function |
| title | The identification of protein and RNA interactors of the splicing factor Caper in the adult Drosophila nervous system |
| title_full | The identification of protein and RNA interactors of the splicing factor Caper in the adult Drosophila nervous system |
| title_fullStr | The identification of protein and RNA interactors of the splicing factor Caper in the adult Drosophila nervous system |
| title_full_unstemmed | The identification of protein and RNA interactors of the splicing factor Caper in the adult Drosophila nervous system |
| title_short | The identification of protein and RNA interactors of the splicing factor Caper in the adult Drosophila nervous system |
| title_sort | identification of protein and rna interactors of the splicing factor caper in the adult drosophila nervous system |
| topic | alternative splicing RNA-binding proteins caper RBM39 Drosophila tissue specific function |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2023.1114857/full |
| work_keys_str_mv | AT mbrandontitus theidentificationofproteinandrnainteractorsofthesplicingfactorcaperintheadultdrosophilanervoussystem AT adelinewchang theidentificationofproteinandrnainteractorsofthesplicingfactorcaperintheadultdrosophilanervoussystem AT nikopopitsch theidentificationofproteinandrnainteractorsofthesplicingfactorcaperintheadultdrosophilanervoussystem AT christophercebmeier theidentificationofproteinandrnainteractorsofthesplicingfactorcaperintheadultdrosophilanervoussystem AT jeremymbono theidentificationofproteinandrnainteractorsofthesplicingfactorcaperintheadultdrosophilanervoussystem AT eugeniacolesnicky theidentificationofproteinandrnainteractorsofthesplicingfactorcaperintheadultdrosophilanervoussystem AT mbrandontitus identificationofproteinandrnainteractorsofthesplicingfactorcaperintheadultdrosophilanervoussystem AT adelinewchang identificationofproteinandrnainteractorsofthesplicingfactorcaperintheadultdrosophilanervoussystem AT nikopopitsch identificationofproteinandrnainteractorsofthesplicingfactorcaperintheadultdrosophilanervoussystem AT christophercebmeier identificationofproteinandrnainteractorsofthesplicingfactorcaperintheadultdrosophilanervoussystem AT jeremymbono identificationofproteinandrnainteractorsofthesplicingfactorcaperintheadultdrosophilanervoussystem AT eugeniacolesnicky identificationofproteinandrnainteractorsofthesplicingfactorcaperintheadultdrosophilanervoussystem |