In Vitro Screening for Anti-Acetylcholinesterase and Antioxidant Activities of <i>Hottonia palustris</i> L. Extracts and Their Unusual Flavonoids
<i>Hottonia palustris</i> L. is from the genus <i>Hottonia</i> (Primulaceae), and the understanding of its phytochemical and pharmacological properties is limited. In this study, the use of chromatographic techniques led to the isolation of a further eleven compounds, includi...
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MDPI AG
2022-11-01
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| Series: | Molecules |
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| author | Jakub W. Strawa Katarzyna Jakimiuk Zuzanna Kita Michał Tomczyk |
| author_facet | Jakub W. Strawa Katarzyna Jakimiuk Zuzanna Kita Michał Tomczyk |
| author_sort | Jakub W. Strawa |
| collection | DOAJ |
| description | <i>Hottonia palustris</i> L. is from the genus <i>Hottonia</i> (Primulaceae), and the understanding of its phytochemical and pharmacological properties is limited. In this study, the use of chromatographic techniques led to the isolation of a further eleven compounds, including three new flavonoids: 2′,5-dihydroxyflavone 2′-<i>O</i>-<i>β</i>-glucopyranoside, 5,6-dihydroxyflavone 6-<i>O</i>-(6”-<i>O</i>-glucopyranosyl)-<i>β</i>-glucopyranoside (hottonioside A), and 4′,5,7-trihydroxyflavone 7-<i>O</i>-(2”-<i>O</i>-<i>β</i>-glucuronide)-<i>β</i>-glucopyranoside. Their structures were determined using extensive 1D and 2D NMR data and mass spectrometry (HRMS). The qualitative assessment of the chemical composition of the investigated extracts and fractions was performed using the LC-HRMS technique. Furthermore, the antioxidant potential of extracts, fractions, and compounds and their ability to inhibit acetylcholinesterase were also evaluated. Thus, we may conclude that the observed biological effects are the result of the presence of many biologically active compounds, of which dibenzoylmethane is the most active. Therefore, <i>H. palustris</i> is a source of substances with desirable properties in the prevention and treatment of neurodegenerative diseases. |
| first_indexed | 2024-03-09T18:06:53Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:06:53Z |
| publishDate | 2022-11-01 |
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| series | Molecules |
| spelling | doaj.art-5dd37480ce6a4f4eaa9186339800ca762023-11-24T09:25:23ZengMDPI AGMolecules1420-30492022-11-012722803410.3390/molecules27228034In Vitro Screening for Anti-Acetylcholinesterase and Antioxidant Activities of <i>Hottonia palustris</i> L. Extracts and Their Unusual FlavonoidsJakub W. Strawa0Katarzyna Jakimiuk1Zuzanna Kita2Michał Tomczyk3Department of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, PolandDepartment of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, PolandStudents’ Scientific Association, Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Bialystok, ul. Mickiewicza 2a, 15-230 Białystok, PolandDepartment of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland<i>Hottonia palustris</i> L. is from the genus <i>Hottonia</i> (Primulaceae), and the understanding of its phytochemical and pharmacological properties is limited. In this study, the use of chromatographic techniques led to the isolation of a further eleven compounds, including three new flavonoids: 2′,5-dihydroxyflavone 2′-<i>O</i>-<i>β</i>-glucopyranoside, 5,6-dihydroxyflavone 6-<i>O</i>-(6”-<i>O</i>-glucopyranosyl)-<i>β</i>-glucopyranoside (hottonioside A), and 4′,5,7-trihydroxyflavone 7-<i>O</i>-(2”-<i>O</i>-<i>β</i>-glucuronide)-<i>β</i>-glucopyranoside. Their structures were determined using extensive 1D and 2D NMR data and mass spectrometry (HRMS). The qualitative assessment of the chemical composition of the investigated extracts and fractions was performed using the LC-HRMS technique. Furthermore, the antioxidant potential of extracts, fractions, and compounds and their ability to inhibit acetylcholinesterase were also evaluated. Thus, we may conclude that the observed biological effects are the result of the presence of many biologically active compounds, of which dibenzoylmethane is the most active. Therefore, <i>H. palustris</i> is a source of substances with desirable properties in the prevention and treatment of neurodegenerative diseases.https://www.mdpi.com/1420-3049/27/22/8034<i>Hottonia palustris</i>PrimulaceaeflavonoidsLC-HRMSantioxidant activityacetylcholinesterase inhibition |
| spellingShingle | Jakub W. Strawa Katarzyna Jakimiuk Zuzanna Kita Michał Tomczyk In Vitro Screening for Anti-Acetylcholinesterase and Antioxidant Activities of <i>Hottonia palustris</i> L. Extracts and Their Unusual Flavonoids Molecules <i>Hottonia palustris</i> Primulaceae flavonoids LC-HRMS antioxidant activity acetylcholinesterase inhibition |
| title | In Vitro Screening for Anti-Acetylcholinesterase and Antioxidant Activities of <i>Hottonia palustris</i> L. Extracts and Their Unusual Flavonoids |
| title_full | In Vitro Screening for Anti-Acetylcholinesterase and Antioxidant Activities of <i>Hottonia palustris</i> L. Extracts and Their Unusual Flavonoids |
| title_fullStr | In Vitro Screening for Anti-Acetylcholinesterase and Antioxidant Activities of <i>Hottonia palustris</i> L. Extracts and Their Unusual Flavonoids |
| title_full_unstemmed | In Vitro Screening for Anti-Acetylcholinesterase and Antioxidant Activities of <i>Hottonia palustris</i> L. Extracts and Their Unusual Flavonoids |
| title_short | In Vitro Screening for Anti-Acetylcholinesterase and Antioxidant Activities of <i>Hottonia palustris</i> L. Extracts and Their Unusual Flavonoids |
| title_sort | in vitro screening for anti acetylcholinesterase and antioxidant activities of i hottonia palustris i l extracts and their unusual flavonoids |
| topic | <i>Hottonia palustris</i> Primulaceae flavonoids LC-HRMS antioxidant activity acetylcholinesterase inhibition |
| url | https://www.mdpi.com/1420-3049/27/22/8034 |
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