Centriolar remodeling underlies basal body maturation during ciliogenesis in Caenorhabditis elegans
The primary cilium is nucleated by the mother centriole-derived basal body (BB) via as yet poorly characterized mechanisms. BBs have been reported to degenerate following ciliogenesis in the C. elegans embryo, although neither BB architecture nor early ciliogenesis steps have been described in this...
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| Format: | Article |
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eLife Sciences Publications Ltd
2017-04-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/25686 |
| _version_ | 1811181455901982720 |
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| author | Inna V Nechipurenko Cristina Berciu Piali Sengupta Daniela Nicastro |
| author_facet | Inna V Nechipurenko Cristina Berciu Piali Sengupta Daniela Nicastro |
| author_sort | Inna V Nechipurenko |
| collection | DOAJ |
| description | The primary cilium is nucleated by the mother centriole-derived basal body (BB) via as yet poorly characterized mechanisms. BBs have been reported to degenerate following ciliogenesis in the C. elegans embryo, although neither BB architecture nor early ciliogenesis steps have been described in this organism. In a previous study (Doroquez et al., 2014), we described the three-dimensional morphologies of sensory neuron cilia in adult C. elegans hermaphrodites at high resolution. Here, we use serial section electron microscopy and tomography of staged C. elegans embryos to demonstrate that BBs remodel to support ciliogenesis in a subset of sensory neurons. We show that centriolar singlet microtubules are converted into BB doublets which subsequently grow asynchronously to template the ciliary axoneme, visualize degeneration of the centriole core, and define the developmental stage at which the transition zone is established. Our work provides a framework for future investigations into the mechanisms underlying BB remodeling. |
| first_indexed | 2024-04-11T09:18:02Z |
| format | Article |
| id | doaj.art-5dd61b6efa3245f88d2d0eab763ed90f |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:18:02Z |
| publishDate | 2017-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5dd61b6efa3245f88d2d0eab763ed90f2022-12-22T04:32:17ZengeLife Sciences Publications LtdeLife2050-084X2017-04-01610.7554/eLife.25686Centriolar remodeling underlies basal body maturation during ciliogenesis in Caenorhabditis elegansInna V Nechipurenko0https://orcid.org/0000-0003-0249-6620Cristina Berciu1Piali Sengupta2https://orcid.org/0000-0001-7468-0035Daniela Nicastro3https://orcid.org/0000-0002-0122-7173Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United StatesDepartment of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, United StatesDepartment of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United StatesDepartment of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, United States; Departments of Cell Biology and Biophysics, University of Texas Southwestern Medical Center, Dallas, United StatesThe primary cilium is nucleated by the mother centriole-derived basal body (BB) via as yet poorly characterized mechanisms. BBs have been reported to degenerate following ciliogenesis in the C. elegans embryo, although neither BB architecture nor early ciliogenesis steps have been described in this organism. In a previous study (Doroquez et al., 2014), we described the three-dimensional morphologies of sensory neuron cilia in adult C. elegans hermaphrodites at high resolution. Here, we use serial section electron microscopy and tomography of staged C. elegans embryos to demonstrate that BBs remodel to support ciliogenesis in a subset of sensory neurons. We show that centriolar singlet microtubules are converted into BB doublets which subsequently grow asynchronously to template the ciliary axoneme, visualize degeneration of the centriole core, and define the developmental stage at which the transition zone is established. Our work provides a framework for future investigations into the mechanisms underlying BB remodeling.https://elifesciences.org/articles/25686ciliabasal bodieselectron microscopy |
| spellingShingle | Inna V Nechipurenko Cristina Berciu Piali Sengupta Daniela Nicastro Centriolar remodeling underlies basal body maturation during ciliogenesis in Caenorhabditis elegans eLife cilia basal bodies electron microscopy |
| title | Centriolar remodeling underlies basal body maturation during ciliogenesis in Caenorhabditis elegans |
| title_full | Centriolar remodeling underlies basal body maturation during ciliogenesis in Caenorhabditis elegans |
| title_fullStr | Centriolar remodeling underlies basal body maturation during ciliogenesis in Caenorhabditis elegans |
| title_full_unstemmed | Centriolar remodeling underlies basal body maturation during ciliogenesis in Caenorhabditis elegans |
| title_short | Centriolar remodeling underlies basal body maturation during ciliogenesis in Caenorhabditis elegans |
| title_sort | centriolar remodeling underlies basal body maturation during ciliogenesis in caenorhabditis elegans |
| topic | cilia basal bodies electron microscopy |
| url | https://elifesciences.org/articles/25686 |
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