Intraspecific Hybridisation of Boronia heterophylla F. Muell
Boronia heterophylla is cultivated for cut flower production. Three cultivars dominate production, ‘Red’, ‘Cameo’, and ‘Moonglow’. A variety of colors and an extended flowering period are demanded by local and overseas markets. The aim of this study was to develop procedures for a Boronia heterophyl...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2011-09-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://journal.ipb.ac.id/index.php/hayati/article/view/3816/2616 |
| Summary: | Boronia heterophylla is cultivated for cut flower production. Three cultivars dominate production, ‘Red’, ‘Cameo’, and ‘Moonglow’. A variety of colors and an extended flowering period are demanded by local and overseas markets. The aim of this study was to develop procedures for a Boronia heterophylla breeding program through intraspesific hybridization. This may yield progeny with desirable characteristics, ideally increased vigor, and a range of flower colors and flowering times. Nine pollination combinations were attempted, each self pollination and all reciprocal crosses. Seed set varied from 17 to 95%. Embryo rescue (was employed to produce hybrid plants using half strength Murashige and Skoog (MS) basal media and it was most successful (75%) 5-6 weeks after pollination. All shoots multiplied on media containing MS salts + NAA (0.1 mg/l) + BA (0.4 mg/l). All shoots transferred to medium containing half strength MS salts + NAA (4 mg/l) produced roots. Plantlets were acclimatized to sterile potting mix (in a small chamber within a glass house. This intraspecific hybridization system was very successful and plants were obtained for all pollination treatments except for selfed ‘Moonglow’. Embryo rescue may provide a system for germinating other species with difficult to germinate or dormant seed. |
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| ISSN: | 1978-3019 2086-4094 |