Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture
<p>Abstract</p> <p>Background</p> <p>Open reading frame 58 (ORF58) of varicella-zoster virus (VZV) lies at the 3'end of the Unique long (U<sub>L</sub>) region and its functional is unknown. In order to clarify whether ORF58 is essential for the growth o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-04-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/5/1/54 |
| _version_ | 1818514341732810752 |
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| author | Nagaike Kazuhiro Matsuura Masaaki Sadaoka Kay Yoshii Hironori Takahashi Michiaki Yamanishi Koichi Mori Yasuko |
| author_facet | Nagaike Kazuhiro Matsuura Masaaki Sadaoka Kay Yoshii Hironori Takahashi Michiaki Yamanishi Koichi Mori Yasuko |
| author_sort | Nagaike Kazuhiro |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Open reading frame 58 (ORF58) of varicella-zoster virus (VZV) lies at the 3'end of the Unique long (U<sub>L</sub>) region and its functional is unknown. In order to clarify whether ORF58 is essential for the growth of VZV, we constructed a deletion mutant of ORF58 (pOka-BACΔ58) from the Oka parental genome cloned into a bacterial artificial chromosome (pOka-BAC).</p> <p>Results</p> <p>The ORF58-deleted virus (rpOkaΔ58) was reconstituted from the pOka-BACΔ58 genome in MRC-5 cells, indicating that the ORF58 gene is non-essential for virus growth. Comparison of the growth rate of rpOkaΔ58 and recombinant wild-type virus by assessing plaque sizes revealed no significant differences between them both in MRC-5 cells and malignant melanoma cells.</p> <p>Conclusion</p> <p>This study shows that the ORF58 gene is dispensable for viral replication and does not affect the virus' ability to form plaques <it>in vitro</it>.</p> |
| first_indexed | 2024-12-11T00:14:21Z |
| format | Article |
| id | doaj.art-5dda23567e3f4ee1a167612a6eaf18f9 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-11T00:14:21Z |
| publishDate | 2008-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-5dda23567e3f4ee1a167612a6eaf18f92022-12-22T01:28:00ZengBMCVirology Journal1743-422X2008-04-01515410.1186/1743-422X-5-54Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell cultureNagaike KazuhiroMatsuura MasaakiSadaoka KayYoshii HironoriTakahashi MichiakiYamanishi KoichiMori Yasuko<p>Abstract</p> <p>Background</p> <p>Open reading frame 58 (ORF58) of varicella-zoster virus (VZV) lies at the 3'end of the Unique long (U<sub>L</sub>) region and its functional is unknown. In order to clarify whether ORF58 is essential for the growth of VZV, we constructed a deletion mutant of ORF58 (pOka-BACΔ58) from the Oka parental genome cloned into a bacterial artificial chromosome (pOka-BAC).</p> <p>Results</p> <p>The ORF58-deleted virus (rpOkaΔ58) was reconstituted from the pOka-BACΔ58 genome in MRC-5 cells, indicating that the ORF58 gene is non-essential for virus growth. Comparison of the growth rate of rpOkaΔ58 and recombinant wild-type virus by assessing plaque sizes revealed no significant differences between them both in MRC-5 cells and malignant melanoma cells.</p> <p>Conclusion</p> <p>This study shows that the ORF58 gene is dispensable for viral replication and does not affect the virus' ability to form plaques <it>in vitro</it>.</p>http://www.virologyj.com/content/5/1/54 |
| spellingShingle | Nagaike Kazuhiro Matsuura Masaaki Sadaoka Kay Yoshii Hironori Takahashi Michiaki Yamanishi Koichi Mori Yasuko Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture Virology Journal |
| title | Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture |
| title_full | Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture |
| title_fullStr | Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture |
| title_full_unstemmed | Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture |
| title_short | Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture |
| title_sort | varicella zoster virus orf 58 gene is dispensable for viral replication in cell culture |
| url | http://www.virologyj.com/content/5/1/54 |
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