Association of long non-coding RNAs and ABO blood groups with acute lymphoblastic leukemia in Egyptian children
Acute lymphoblastic leukemia (ALL) is the most prevailing cancer among children. Despite extensive studies, ALL etiology is still an unsolved puzzle. Long non-coding RNAs (lncRNAs) emerged as key mediators in cancer etiology. Several lncRNAs are dysregulated in ALL, leading to oncogenic or tumor-sup...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-06-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054024000106 |
| _version_ | 1797234040208621568 |
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| author | Noha H. Sayed Mahmoud Hammad Safeya A. Abdelrahman Hanan M. Abdelgawad |
| author_facet | Noha H. Sayed Mahmoud Hammad Safeya A. Abdelrahman Hanan M. Abdelgawad |
| author_sort | Noha H. Sayed |
| collection | DOAJ |
| description | Acute lymphoblastic leukemia (ALL) is the most prevailing cancer among children. Despite extensive studies, ALL etiology is still an unsolved puzzle. Long non-coding RNAs (lncRNAs) emerged as key mediators in cancer etiology. Several lncRNAs are dysregulated in ALL, leading to oncogenic or tumor-suppressive activities. Additionally, a relation between ABO blood groups and hematological malignancies was proposed. The current study intended to explore the association of lncRNAs, ANRIL and LINC-PINT, and their downstream targets, CDKN2A and heme oxygenase-1 (HMOX1), with the incidence of ALL and treatment response, and to determine the distribution of blood groups across different childhood ALL phenotypes. Blood samples were taken from 66 ALL patients (at diagnosis and at the end of remission induction phase) and 39 healthy children. Whole blood was used for blood group typing. Expression of ANRIL, LINC-PINT and CDKN2A was analyzed in plasma by qRT-PCR. Serum HMOX1 was measured using ELISA. ANRIL and CDKN2A were upregulated, while LINC-PINT and HMOX1 were downregulated in newly diagnosed patients. All of which showed remarkable diagnostic performance, where HMOX1 was superior. HMOX1 was independent predictor of ALL as well. LINC-PINT and HMOX1 were significantly upregulated after treatment. Notably, ANRIL and LINC-PINT were associated with poor outcome. No significant difference in the distribution of ABO blood groups was observed between patients and controls. In conclusion, our results suggested an association of ANRIL and LINC-PINT with childhood ALL predisposition, at least in part, through altering CDKN2A and HMOX1 production. Furthermore, the impact of remission induction treatment was newly revealed. |
| first_indexed | 2024-04-24T16:25:44Z |
| format | Article |
| id | doaj.art-5ddc162f11ac4cdfa15a2ab5fbf5e2a3 |
| institution | Directory Open Access Journal |
| issn | 2468-0540 |
| language | English |
| last_indexed | 2024-04-24T16:25:44Z |
| publishDate | 2024-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Non-coding RNA Research |
| spelling | doaj.art-5ddc162f11ac4cdfa15a2ab5fbf5e2a32024-03-31T04:37:47ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402024-06-0192307317Association of long non-coding RNAs and ABO blood groups with acute lymphoblastic leukemia in Egyptian childrenNoha H. Sayed0Mahmoud Hammad1Safeya A. Abdelrahman2Hanan M. Abdelgawad3Biochemistry Department, Faculty of Pharmacy, Cairo University, Egypt; Corresponding author.Pediatric Oncology Department, National Cancer Institute, Cairo University, EgyptBiochemistry Department, Faculty of Pharmacy, Cairo University, EgyptBiochemistry Department, Faculty of Pharmacy, Cairo University, EgyptAcute lymphoblastic leukemia (ALL) is the most prevailing cancer among children. Despite extensive studies, ALL etiology is still an unsolved puzzle. Long non-coding RNAs (lncRNAs) emerged as key mediators in cancer etiology. Several lncRNAs are dysregulated in ALL, leading to oncogenic or tumor-suppressive activities. Additionally, a relation between ABO blood groups and hematological malignancies was proposed. The current study intended to explore the association of lncRNAs, ANRIL and LINC-PINT, and their downstream targets, CDKN2A and heme oxygenase-1 (HMOX1), with the incidence of ALL and treatment response, and to determine the distribution of blood groups across different childhood ALL phenotypes. Blood samples were taken from 66 ALL patients (at diagnosis and at the end of remission induction phase) and 39 healthy children. Whole blood was used for blood group typing. Expression of ANRIL, LINC-PINT and CDKN2A was analyzed in plasma by qRT-PCR. Serum HMOX1 was measured using ELISA. ANRIL and CDKN2A were upregulated, while LINC-PINT and HMOX1 were downregulated in newly diagnosed patients. All of which showed remarkable diagnostic performance, where HMOX1 was superior. HMOX1 was independent predictor of ALL as well. LINC-PINT and HMOX1 were significantly upregulated after treatment. Notably, ANRIL and LINC-PINT were associated with poor outcome. No significant difference in the distribution of ABO blood groups was observed between patients and controls. In conclusion, our results suggested an association of ANRIL and LINC-PINT with childhood ALL predisposition, at least in part, through altering CDKN2A and HMOX1 production. Furthermore, the impact of remission induction treatment was newly revealed.http://www.sciencedirect.com/science/article/pii/S2468054024000106ABO blood groupsANRILCDKN2AChildhood ALLHMOX1LINC-PINT |
| spellingShingle | Noha H. Sayed Mahmoud Hammad Safeya A. Abdelrahman Hanan M. Abdelgawad Association of long non-coding RNAs and ABO blood groups with acute lymphoblastic leukemia in Egyptian children Non-coding RNA Research ABO blood groups ANRIL CDKN2A Childhood ALL HMOX1 LINC-PINT |
| title | Association of long non-coding RNAs and ABO blood groups with acute lymphoblastic leukemia in Egyptian children |
| title_full | Association of long non-coding RNAs and ABO blood groups with acute lymphoblastic leukemia in Egyptian children |
| title_fullStr | Association of long non-coding RNAs and ABO blood groups with acute lymphoblastic leukemia in Egyptian children |
| title_full_unstemmed | Association of long non-coding RNAs and ABO blood groups with acute lymphoblastic leukemia in Egyptian children |
| title_short | Association of long non-coding RNAs and ABO blood groups with acute lymphoblastic leukemia in Egyptian children |
| title_sort | association of long non coding rnas and abo blood groups with acute lymphoblastic leukemia in egyptian children |
| topic | ABO blood groups ANRIL CDKN2A Childhood ALL HMOX1 LINC-PINT |
| url | http://www.sciencedirect.com/science/article/pii/S2468054024000106 |
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