Comparative analysis of B2L genes in vaccine and wild strains of orf virus in China
Orf is highly contagious zoonoses which is caused by the orf virus (ORFV). In order to determine the characteristics of B2L genes between vaccine strain and wild strain of ORFV in China. In this study we choose the weak vaccine of ORFV produced in biological products (Shandong Taifeng, China) and th...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Council of Agricultural Research
2021-04-01
|
| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/111248 |
| _version_ | 1828005951560482816 |
|---|---|
| author | YU-SHENG LIN JIN-XIU JIANG |
| author_facet | YU-SHENG LIN JIN-XIU JIANG |
| author_sort | YU-SHENG LIN |
| collection | DOAJ |
| description | Orf is highly contagious zoonoses which is caused by the orf virus (ORFV). In order to determine the characteristics of B2L genes between vaccine strain and wild strain of ORFV in China. In this study we choose the weak vaccine of ORFV produced in biological products (Shandong Taifeng, China) and the wild strain (FJ-PN) isolated and identified in our laboratory to amplify, clone and sequence the B2L genes, and the changes in nucleotide, amino acid and secondary and tertiary protein structure of the B2L proteins were compared and analyzed by bioinformatics software. Comparison results showed that the nucleotide sequence similarity and amino acid sequence similarity of the B2L genes of vaccine strain and wild strain were 98.6% and 98.7% respectively. There was no significant difference between the primary, secondary and tertiary structures of the B2L proteins. The results of this study provide ideas for the study on the function and molecular biological characteristics of related proteins after the ORFV is weakened. |
| first_indexed | 2024-04-10T07:49:49Z |
| format | Article |
| id | doaj.art-5ddcf137f02b4ff29115fd06514eb8f3 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:49:49Z |
| publishDate | 2021-04-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-5ddcf137f02b4ff29115fd06514eb8f32023-02-23T10:20:52ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272021-04-01901010.56093/ijans.v90i10.111248Comparative analysis of B2L genes in vaccine and wild strains of orf virus in ChinaYU-SHENG LIN0JIN-XIU JIANG1Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, PR ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, PR ChinaOrf is highly contagious zoonoses which is caused by the orf virus (ORFV). In order to determine the characteristics of B2L genes between vaccine strain and wild strain of ORFV in China. In this study we choose the weak vaccine of ORFV produced in biological products (Shandong Taifeng, China) and the wild strain (FJ-PN) isolated and identified in our laboratory to amplify, clone and sequence the B2L genes, and the changes in nucleotide, amino acid and secondary and tertiary protein structure of the B2L proteins were compared and analyzed by bioinformatics software. Comparison results showed that the nucleotide sequence similarity and amino acid sequence similarity of the B2L genes of vaccine strain and wild strain were 98.6% and 98.7% respectively. There was no significant difference between the primary, secondary and tertiary structures of the B2L proteins. The results of this study provide ideas for the study on the function and molecular biological characteristics of related proteins after the ORFV is weakened.https://epubs.icar.org.in/index.php/IJAnS/article/view/111248Orf virusB2L geneVaccine strainWild strain |
| spellingShingle | YU-SHENG LIN JIN-XIU JIANG Comparative analysis of B2L genes in vaccine and wild strains of orf virus in China Indian Journal of Animal Sciences Orf virus B2L gene Vaccine strain Wild strain |
| title | Comparative analysis of B2L genes in vaccine and wild strains of orf virus in China |
| title_full | Comparative analysis of B2L genes in vaccine and wild strains of orf virus in China |
| title_fullStr | Comparative analysis of B2L genes in vaccine and wild strains of orf virus in China |
| title_full_unstemmed | Comparative analysis of B2L genes in vaccine and wild strains of orf virus in China |
| title_short | Comparative analysis of B2L genes in vaccine and wild strains of orf virus in China |
| title_sort | comparative analysis of b2l genes in vaccine and wild strains of orf virus in china |
| topic | Orf virus B2L gene Vaccine strain Wild strain |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/111248 |
| work_keys_str_mv | AT yushenglin comparativeanalysisofb2lgenesinvaccineandwildstrainsoforfvirusinchina AT jinxiujiang comparativeanalysisofb2lgenesinvaccineandwildstrainsoforfvirusinchina |