Single opsin driven white noise ERGs in mice
PurposeElectroretinograms elicited by photopigment isolating white noise stimuli (wnERGs) in mice were measured. The dependency of rod- and cone-opsin-driven wnERGs on mean luminance was studied.MethodsTemporal white noise stimuli (containing all frequencies up to 20 Hz, equal amplitudes, random pha...
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2023.1211329/full |
| _version_ | 1827889606150848512 |
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| author | Nina Stallwitz Nina Stallwitz Anneka Joachimsthaler Anneka Joachimsthaler Jan Kremers |
| author_facet | Nina Stallwitz Nina Stallwitz Anneka Joachimsthaler Anneka Joachimsthaler Jan Kremers |
| author_sort | Nina Stallwitz |
| collection | DOAJ |
| description | PurposeElectroretinograms elicited by photopigment isolating white noise stimuli (wnERGs) in mice were measured. The dependency of rod- and cone-opsin-driven wnERGs on mean luminance was studied.MethodsTemporal white noise stimuli (containing all frequencies up to 20 Hz, equal amplitudes, random phases) that modulated either rhodopsin, S-opsin or L*-opsin, using the double silent substitution technique, were used to record wnERGs in mice expressing a human L*-opsin instead of the native murine M-opsin. Responses were recorded at 4 mean luminances (MLs).Impulse response functions (IRFs) were obtained by cross-correlating the wnERG recordings with the corresponding modulation of the photopigment excitation elicited by the stimulus. So-called modulation transfer functions (MTFs) were obtained by performing a Fourier transform on the IRFs.Potentials of two repeated wnERG recordings at corresponding time points were plotted against each other. The correlation coefficient (r2repr) of the linear regression through these data was used to quantify reproducibility. Another correlation coefficient (r2ML) was used to quantify the correlations of the wnERGs obtained at different MLs with those at the highest (for cone isolating stimuli) or lowest (for rod isolating stimuli) ML.ResultsIRFs showed an initial negative (a-wave like) trough N1 and a subsequent positive (b-wave like) peak P1. No oscillatory potential-like components were observed. At 0.4 and 1.0 log cd/m2 ML robust L*- and S-opsin-driven IRFs were obtained that displayed similar latencies and dependencies on ML. L*-opsin-driven IRFs were 2.5–3 times larger than S-opsin-driven IRFs. Rhodopsin-driven IRFs were observed at −0.8 and − 0.2 log cd/m2 and decreased in amplitude with increasing ML. They displayed an additional pronounced late negativity (N2), which may be a correlate of retinal ganglion cell activity.R2repr and r2ML values increased for cones with increasing ML whereas they decreased for rods. For rhodopsin-driven MTFs at low MLs and L*-opsin-driven MTFs at high MLs amplitudes decreased with increasing frequency, with much faster decreasing amplitudes for rhodopsin. A delay was calculated from MTF phases showing larger delays for rhodopsin- vs. low delays for L*-opsin-driven responses.ConclusionOpsin-isolating wnERGs in mice show characteristics of different retinal cell types and their connected pathways. |
| first_indexed | 2024-03-12T20:58:01Z |
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| id | doaj.art-5dded5fc55ad45979fd2e824d79a5a4b |
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| language | English |
| last_indexed | 2024-03-12T20:58:01Z |
| publishDate | 2023-07-01 |
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| spelling | doaj.art-5dded5fc55ad45979fd2e824d79a5a4b2023-07-31T11:08:46ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2023-07-011710.3389/fnins.2023.12113291211329Single opsin driven white noise ERGs in miceNina Stallwitz0Nina Stallwitz1Anneka Joachimsthaler2Anneka Joachimsthaler3Jan Kremers4Department of Ophthalmology, University Hospital Erlangen, Erlangen, GermanyAnimal Physiology, Department of Biology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, GermanyDepartment of Ophthalmology, University Hospital Erlangen, Erlangen, GermanyAnimal Physiology, Department of Biology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, GermanyDepartment of Ophthalmology, University Hospital Erlangen, Erlangen, GermanyPurposeElectroretinograms elicited by photopigment isolating white noise stimuli (wnERGs) in mice were measured. The dependency of rod- and cone-opsin-driven wnERGs on mean luminance was studied.MethodsTemporal white noise stimuli (containing all frequencies up to 20 Hz, equal amplitudes, random phases) that modulated either rhodopsin, S-opsin or L*-opsin, using the double silent substitution technique, were used to record wnERGs in mice expressing a human L*-opsin instead of the native murine M-opsin. Responses were recorded at 4 mean luminances (MLs).Impulse response functions (IRFs) were obtained by cross-correlating the wnERG recordings with the corresponding modulation of the photopigment excitation elicited by the stimulus. So-called modulation transfer functions (MTFs) were obtained by performing a Fourier transform on the IRFs.Potentials of two repeated wnERG recordings at corresponding time points were plotted against each other. The correlation coefficient (r2repr) of the linear regression through these data was used to quantify reproducibility. Another correlation coefficient (r2ML) was used to quantify the correlations of the wnERGs obtained at different MLs with those at the highest (for cone isolating stimuli) or lowest (for rod isolating stimuli) ML.ResultsIRFs showed an initial negative (a-wave like) trough N1 and a subsequent positive (b-wave like) peak P1. No oscillatory potential-like components were observed. At 0.4 and 1.0 log cd/m2 ML robust L*- and S-opsin-driven IRFs were obtained that displayed similar latencies and dependencies on ML. L*-opsin-driven IRFs were 2.5–3 times larger than S-opsin-driven IRFs. Rhodopsin-driven IRFs were observed at −0.8 and − 0.2 log cd/m2 and decreased in amplitude with increasing ML. They displayed an additional pronounced late negativity (N2), which may be a correlate of retinal ganglion cell activity.R2repr and r2ML values increased for cones with increasing ML whereas they decreased for rods. For rhodopsin-driven MTFs at low MLs and L*-opsin-driven MTFs at high MLs amplitudes decreased with increasing frequency, with much faster decreasing amplitudes for rhodopsin. A delay was calculated from MTF phases showing larger delays for rhodopsin- vs. low delays for L*-opsin-driven responses.ConclusionOpsin-isolating wnERGs in mice show characteristics of different retinal cell types and their connected pathways.https://www.frontiersin.org/articles/10.3389/fnins.2023.1211329/fullelectroretinography (ERG)mouse retinaphotopigmentsilent substitutiontemporal white noise (TWN) |
| spellingShingle | Nina Stallwitz Nina Stallwitz Anneka Joachimsthaler Anneka Joachimsthaler Jan Kremers Single opsin driven white noise ERGs in mice Frontiers in Neuroscience electroretinography (ERG) mouse retina photopigment silent substitution temporal white noise (TWN) |
| title | Single opsin driven white noise ERGs in mice |
| title_full | Single opsin driven white noise ERGs in mice |
| title_fullStr | Single opsin driven white noise ERGs in mice |
| title_full_unstemmed | Single opsin driven white noise ERGs in mice |
| title_short | Single opsin driven white noise ERGs in mice |
| title_sort | single opsin driven white noise ergs in mice |
| topic | electroretinography (ERG) mouse retina photopigment silent substitution temporal white noise (TWN) |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2023.1211329/full |
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