<i>Elettaria cardamomum</i> (L.) Maton Essential Oil: An Interesting Source of Bioactive Specialized Metabolites as Inhibitors of Acetylcholinesterase and Butyrylcholinesterase
<i>Elettaria cardamomum</i> (L.) Maton (Zingiberaceae family) is a plant traditionally used in Ayurvedic and Chinese medicine. In this work, the essential oil of <i>E. cardamomum</i> was found to inhibit the enzymes AChE (62.6% of inhibition, IC<sub>50</sub> 24.9...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/19/3463 |
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| author | Marta Pavarino Arianna Marengo Cecilia Cagliero Carlo Bicchi Patrizia Rubiolo Barbara Sgorbini |
| author_facet | Marta Pavarino Arianna Marengo Cecilia Cagliero Carlo Bicchi Patrizia Rubiolo Barbara Sgorbini |
| author_sort | Marta Pavarino |
| collection | DOAJ |
| description | <i>Elettaria cardamomum</i> (L.) Maton (Zingiberaceae family) is a plant traditionally used in Ayurvedic and Chinese medicine. In this work, the essential oil of <i>E. cardamomum</i> was found to inhibit the enzymes AChE (62.6% of inhibition, IC<sub>50</sub> 24.9 μg/mL) and BChE (55.8% of inhibition, IC<sub>50</sub> 25.9 μg/mL) by performing an in vitro colorimetric assay using the Ellman method. A bio-guided fractionation approach was used to isolate fractions/pure compounds that were tested individually to evaluate their activity. The resulting oxygenated fraction was found to be active against both AChE (percentage inhibition 42.8%) and BChE (percentage inhibition 63.7%), while the hydrocarbon fraction was inactive. The activity was attributed to a pool of oxygenated terpenes (α-terpinyl acetate, 1,8-cineole, linalool, linalyl acetate, and α-terpineol) that synergistically contributed to the overall activity of the essential oil. |
| first_indexed | 2024-03-10T21:37:40Z |
| format | Article |
| id | doaj.art-5de4a94e91b44a358e29404926cffff6 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T21:37:40Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-5de4a94e91b44a358e29404926cffff62023-11-19T14:54:44ZengMDPI AGPlants2223-77472023-10-011219346310.3390/plants12193463<i>Elettaria cardamomum</i> (L.) Maton Essential Oil: An Interesting Source of Bioactive Specialized Metabolites as Inhibitors of Acetylcholinesterase and ButyrylcholinesteraseMarta Pavarino0Arianna Marengo1Cecilia Cagliero2Carlo Bicchi3Patrizia Rubiolo4Barbara Sgorbini5Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via Pietro Giuria 9, I-10125 Turin, ItalyDipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via Pietro Giuria 9, I-10125 Turin, ItalyDipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via Pietro Giuria 9, I-10125 Turin, ItalyDipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via Pietro Giuria 9, I-10125 Turin, ItalyDipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via Pietro Giuria 9, I-10125 Turin, ItalyDipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via Pietro Giuria 9, I-10125 Turin, Italy<i>Elettaria cardamomum</i> (L.) Maton (Zingiberaceae family) is a plant traditionally used in Ayurvedic and Chinese medicine. In this work, the essential oil of <i>E. cardamomum</i> was found to inhibit the enzymes AChE (62.6% of inhibition, IC<sub>50</sub> 24.9 μg/mL) and BChE (55.8% of inhibition, IC<sub>50</sub> 25.9 μg/mL) by performing an in vitro colorimetric assay using the Ellman method. A bio-guided fractionation approach was used to isolate fractions/pure compounds that were tested individually to evaluate their activity. The resulting oxygenated fraction was found to be active against both AChE (percentage inhibition 42.8%) and BChE (percentage inhibition 63.7%), while the hydrocarbon fraction was inactive. The activity was attributed to a pool of oxygenated terpenes (α-terpinyl acetate, 1,8-cineole, linalool, linalyl acetate, and α-terpineol) that synergistically contributed to the overall activity of the essential oil.https://www.mdpi.com/2223-7747/12/19/3463<i>Elettaria cardamomum</i> essential oilacetylcholinesterasebutyrylcholinesterasebio-guided fractionation |
| spellingShingle | Marta Pavarino Arianna Marengo Cecilia Cagliero Carlo Bicchi Patrizia Rubiolo Barbara Sgorbini <i>Elettaria cardamomum</i> (L.) Maton Essential Oil: An Interesting Source of Bioactive Specialized Metabolites as Inhibitors of Acetylcholinesterase and Butyrylcholinesterase Plants <i>Elettaria cardamomum</i> essential oil acetylcholinesterase butyrylcholinesterase bio-guided fractionation |
| title | <i>Elettaria cardamomum</i> (L.) Maton Essential Oil: An Interesting Source of Bioactive Specialized Metabolites as Inhibitors of Acetylcholinesterase and Butyrylcholinesterase |
| title_full | <i>Elettaria cardamomum</i> (L.) Maton Essential Oil: An Interesting Source of Bioactive Specialized Metabolites as Inhibitors of Acetylcholinesterase and Butyrylcholinesterase |
| title_fullStr | <i>Elettaria cardamomum</i> (L.) Maton Essential Oil: An Interesting Source of Bioactive Specialized Metabolites as Inhibitors of Acetylcholinesterase and Butyrylcholinesterase |
| title_full_unstemmed | <i>Elettaria cardamomum</i> (L.) Maton Essential Oil: An Interesting Source of Bioactive Specialized Metabolites as Inhibitors of Acetylcholinesterase and Butyrylcholinesterase |
| title_short | <i>Elettaria cardamomum</i> (L.) Maton Essential Oil: An Interesting Source of Bioactive Specialized Metabolites as Inhibitors of Acetylcholinesterase and Butyrylcholinesterase |
| title_sort | i elettaria cardamomum i l maton essential oil an interesting source of bioactive specialized metabolites as inhibitors of acetylcholinesterase and butyrylcholinesterase |
| topic | <i>Elettaria cardamomum</i> essential oil acetylcholinesterase butyrylcholinesterase bio-guided fractionation |
| url | https://www.mdpi.com/2223-7747/12/19/3463 |
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