Baoyuan Jiedu decoction alleviating cancer cachexia–Induced muscle atrophy by regulating muscle mitochondrial function in ApcMin/+ mice
Cancer cachexia is a complex syndrome that leads to an ongoing loss of skeletal muscle mass in many malignant tumors. Our previous studies have evaluated the effectiveness of Baoyuan Jiedu decoction (BJD) in alleviating cancer-induced muscle atrophy. However, the mechanisms of BJD regulating muscle...
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.914597/full |
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| author | Beiying Zhang Qianyu Bi Shengqi Huang Siyuan Lv Xin Zong Mengran Wang Xuming Ji Xuming Ji |
| author_facet | Beiying Zhang Qianyu Bi Shengqi Huang Siyuan Lv Xin Zong Mengran Wang Xuming Ji Xuming Ji |
| author_sort | Beiying Zhang |
| collection | DOAJ |
| description | Cancer cachexia is a complex syndrome that leads to an ongoing loss of skeletal muscle mass in many malignant tumors. Our previous studies have evaluated the effectiveness of Baoyuan Jiedu decoction (BJD) in alleviating cancer-induced muscle atrophy. However, the mechanisms of BJD regulating muscle atrophy could not be fully understood. Therefore, we further investigated the mechanisms of BJD mitigating muscle atrophy both in an ApcMin/+ mouse model and the Lewis-conditioned medium–induced C2C12 myotube atrophy model. We confirmed the quality of BJD extracts by HPLC. In an In vivo study, body weight loss and muscle atrophy were alleviated with BJD treatment. GO analysis suggested that ATP metabolism and mitochondria were involved. The results of the electron microscope show that BJD treatment may have a healing effect on mitochondrial structure. Moreover, ATP content and mitochondrial numbers were improved with BJD treatment. Furthermore, both in vivo and in vitro, we demonstrated that the BJD treatment could improve mitochondrial function owing to the increased number of mitochondria, balanced dynamic, and regulation of the electron transport chain according to the protein and mRNA expressions. In addition, oxidative stress caused by mitochondrial dysfunction was ameliorated by BJD treatment in ApcMin/+ mice. Consequently, our study provides proof for BJD treatment alleviating cancer cachexia–induced muscle atrophy by modulating mitochondrial function in ApcMin/+ mice. |
| first_indexed | 2024-12-10T15:46:47Z |
| format | Article |
| id | doaj.art-5dea3e278d2f4820869d324faa3eefa1 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-10T15:46:47Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-5dea3e278d2f4820869d324faa3eefa12022-12-22T01:42:56ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-08-011310.3389/fphar.2022.914597914597Baoyuan Jiedu decoction alleviating cancer cachexia–Induced muscle atrophy by regulating muscle mitochondrial function in ApcMin/+ miceBeiying Zhang0Qianyu Bi1Shengqi Huang2Siyuan Lv3Xin Zong4Mengran Wang5Xuming Ji6Xuming Ji7School of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, ChinaWeifang Nursing Vocational College, Weifang, ChinaDepartment of Pediatrics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, ChinaSchool of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, ChinaAcademy of Chinese Medical Science, Zhejiang Chinese Medical University, Hangzhou, ChinaCancer cachexia is a complex syndrome that leads to an ongoing loss of skeletal muscle mass in many malignant tumors. Our previous studies have evaluated the effectiveness of Baoyuan Jiedu decoction (BJD) in alleviating cancer-induced muscle atrophy. However, the mechanisms of BJD regulating muscle atrophy could not be fully understood. Therefore, we further investigated the mechanisms of BJD mitigating muscle atrophy both in an ApcMin/+ mouse model and the Lewis-conditioned medium–induced C2C12 myotube atrophy model. We confirmed the quality of BJD extracts by HPLC. In an In vivo study, body weight loss and muscle atrophy were alleviated with BJD treatment. GO analysis suggested that ATP metabolism and mitochondria were involved. The results of the electron microscope show that BJD treatment may have a healing effect on mitochondrial structure. Moreover, ATP content and mitochondrial numbers were improved with BJD treatment. Furthermore, both in vivo and in vitro, we demonstrated that the BJD treatment could improve mitochondrial function owing to the increased number of mitochondria, balanced dynamic, and regulation of the electron transport chain according to the protein and mRNA expressions. In addition, oxidative stress caused by mitochondrial dysfunction was ameliorated by BJD treatment in ApcMin/+ mice. Consequently, our study provides proof for BJD treatment alleviating cancer cachexia–induced muscle atrophy by modulating mitochondrial function in ApcMin/+ mice.https://www.frontiersin.org/articles/10.3389/fphar.2022.914597/fullcancer cachexiamuscle atrophyBaoyuan Jiedu decoctionmitochondrial functionAPCMin/+ mice |
| spellingShingle | Beiying Zhang Qianyu Bi Shengqi Huang Siyuan Lv Xin Zong Mengran Wang Xuming Ji Xuming Ji Baoyuan Jiedu decoction alleviating cancer cachexia–Induced muscle atrophy by regulating muscle mitochondrial function in ApcMin/+ mice Frontiers in Pharmacology cancer cachexia muscle atrophy Baoyuan Jiedu decoction mitochondrial function APCMin/+ mice |
| title | Baoyuan Jiedu decoction alleviating cancer cachexia–Induced muscle atrophy by regulating muscle mitochondrial function in ApcMin/+ mice |
| title_full | Baoyuan Jiedu decoction alleviating cancer cachexia–Induced muscle atrophy by regulating muscle mitochondrial function in ApcMin/+ mice |
| title_fullStr | Baoyuan Jiedu decoction alleviating cancer cachexia–Induced muscle atrophy by regulating muscle mitochondrial function in ApcMin/+ mice |
| title_full_unstemmed | Baoyuan Jiedu decoction alleviating cancer cachexia–Induced muscle atrophy by regulating muscle mitochondrial function in ApcMin/+ mice |
| title_short | Baoyuan Jiedu decoction alleviating cancer cachexia–Induced muscle atrophy by regulating muscle mitochondrial function in ApcMin/+ mice |
| title_sort | baoyuan jiedu decoction alleviating cancer cachexia induced muscle atrophy by regulating muscle mitochondrial function in apcmin mice |
| topic | cancer cachexia muscle atrophy Baoyuan Jiedu decoction mitochondrial function APCMin/+ mice |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.914597/full |
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