Bioremediation of Soil Microcosms from Auto-Mechanic Workshops

Microbial activities are essential for the restoration of soil contaminated with hydrocarbons, their roles includes biotransformation and mineralization of petroleum products into harmless compounds. In this study eighty six soil samples from fifteen auto-mechanic workshops within Ilorin metropolis...

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Main Authors: AT Ajao, AO Oluwajobi, VS Olatayo
Format: Article
Language:English
Published: Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) 2013-05-01
Series:Journal of Applied Sciences and Environmental Management
Online Access:https://www.ajol.info/index.php/jasem/article/view/88688
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author AT Ajao
AO Oluwajobi
VS Olatayo
author_facet AT Ajao
AO Oluwajobi
VS Olatayo
author_sort AT Ajao
collection DOAJ
description Microbial activities are essential for the restoration of soil contaminated with hydrocarbons, their roles includes biotransformation and mineralization of petroleum products into harmless compounds. In this study eighty six soil samples from fifteen auto-mechanic workshops within Ilorin metropolis were collected. These were analyzed by selective enrichment technique, resulted in the isolation of five bacterial species which are Acinetobacter sp, Flavobacterium sp, Pseudomonas sp., Serratia and Bacillus sp. Bioremediation of the soil microcosms was designed for 2months using mixed culture of the isolates. TVC, Protein, Dehydrogenase and lipase activities were used as bioindicators for the bioremediation processes. Highest TVC, Protein and Dehydrogenase activities were recorded in four week as 163.15μgTPFg-1soil,6.3×107and5.53mg/g were obtained respectively at pH of 7.17 and gradually declined while lipase activities and percentage of oil was highest in week six as 65.41% and4.72unit/g. These findings have environmental implication towards developing a bioremediation protocol that could be exploited for cleaning oil polluted soil @JASEM Keywords: Bioremediation, automechanic workshop, Bioindicator, Hydrocarbon, Selective enrichment J. Appl. Sci. Environ. Manage. Sept, 2011, Vol. 15 (3) 473 -477
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spelling doaj.art-5dea51812c92413bb6fe41bbc128ce1a2024-04-22T19:46:56ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992013-05-01153Bioremediation of Soil Microcosms from Auto-Mechanic WorkshopsAT AjaoAO OluwajobiVS OlatayoMicrobial activities are essential for the restoration of soil contaminated with hydrocarbons, their roles includes biotransformation and mineralization of petroleum products into harmless compounds. In this study eighty six soil samples from fifteen auto-mechanic workshops within Ilorin metropolis were collected. These were analyzed by selective enrichment technique, resulted in the isolation of five bacterial species which are Acinetobacter sp, Flavobacterium sp, Pseudomonas sp., Serratia and Bacillus sp. Bioremediation of the soil microcosms was designed for 2months using mixed culture of the isolates. TVC, Protein, Dehydrogenase and lipase activities were used as bioindicators for the bioremediation processes. Highest TVC, Protein and Dehydrogenase activities were recorded in four week as 163.15μgTPFg-1soil,6.3×107and5.53mg/g were obtained respectively at pH of 7.17 and gradually declined while lipase activities and percentage of oil was highest in week six as 65.41% and4.72unit/g. These findings have environmental implication towards developing a bioremediation protocol that could be exploited for cleaning oil polluted soil @JASEM Keywords: Bioremediation, automechanic workshop, Bioindicator, Hydrocarbon, Selective enrichment J. Appl. Sci. Environ. Manage. Sept, 2011, Vol. 15 (3) 473 -477https://www.ajol.info/index.php/jasem/article/view/88688
spellingShingle AT Ajao
AO Oluwajobi
VS Olatayo
Bioremediation of Soil Microcosms from Auto-Mechanic Workshops
Journal of Applied Sciences and Environmental Management
title Bioremediation of Soil Microcosms from Auto-Mechanic Workshops
title_full Bioremediation of Soil Microcosms from Auto-Mechanic Workshops
title_fullStr Bioremediation of Soil Microcosms from Auto-Mechanic Workshops
title_full_unstemmed Bioremediation of Soil Microcosms from Auto-Mechanic Workshops
title_short Bioremediation of Soil Microcosms from Auto-Mechanic Workshops
title_sort bioremediation of soil microcosms from auto mechanic workshops
url https://www.ajol.info/index.php/jasem/article/view/88688
work_keys_str_mv AT atajao bioremediationofsoilmicrocosmsfromautomechanicworkshops
AT aooluwajobi bioremediationofsoilmicrocosmsfromautomechanicworkshops
AT vsolatayo bioremediationofsoilmicrocosmsfromautomechanicworkshops