Wound Healing Effects of <i>Aloe muth-muth</i>: In Vitro Investigations Using Immortalized Human Keratinocytes (HaCaT)
The traditional use of <i>Aloe</i> spp. for the purpose of wound healing has a long history and is widespread internationally. Recently, a hybrid aloe plant (<i>Aloe muth-muth</i>) has been cultivated by cross pollination between <i>Aloe vera</i> and <i>Aloe...
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MDPI AG
2020-10-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/9/11/350 |
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| author | Morné Fouché Clarissa Willers Sias Hamman Christiaan Malherbe Jan Steenekamp |
| author_facet | Morné Fouché Clarissa Willers Sias Hamman Christiaan Malherbe Jan Steenekamp |
| author_sort | Morné Fouché |
| collection | DOAJ |
| description | The traditional use of <i>Aloe</i> spp. for the purpose of wound healing has a long history and is widespread internationally. Recently, a hybrid aloe plant (<i>Aloe muth-muth</i>) has been cultivated by cross pollination between <i>Aloe vera</i> and <i>Aloe ferox</i>. The <i>Aloe muth-muth</i> plant has not yet been investigated for medicinal properties and provides an opportunity for potential biological activity, including wound healing. The aim of this study was to investigate the in vitro wound healing effects of both <i>Aloe muth-muth</i> gel and whole leaf material with the use of the immortalized human keratinocyte (HaCaT) cell line. Cell viability was conducted using methyl thiazolyl tetrazolium (MTT) assays. In vitro wound healing was tested on HaCaT cells using an established scratch assay method. The effect of <i>Aloe muth-muth</i> gel material on HaCaT cell migration was also investigated. <i>Aloe muth-muth</i> gel material exhibited statistically significantly (<i>p</i> < 0.05) higher percentage wound closure compared to the control at all three concentrations investigated. These findings confirm that this newly cultivated species, <i>Aloe muth-muth</i>, also possesses wound healing activity corresponding to that reported for the two species it is derived from, namely, <i>Aloe vera</i> and <i>Aloe ferox</i>. Therefore, <i>Aloe muth-muth</i> has the potential to be used in future wound therapeutics. |
| first_indexed | 2024-03-10T15:23:36Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T15:23:36Z |
| publishDate | 2020-10-01 |
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| record_format | Article |
| series | Biology |
| spelling | doaj.art-5deb95b0c34c46598c40de504acc2f2a2023-11-20T18:12:56ZengMDPI AGBiology2079-77372020-10-0191135010.3390/biology9110350Wound Healing Effects of <i>Aloe muth-muth</i>: In Vitro Investigations Using Immortalized Human Keratinocytes (HaCaT)Morné Fouché0Clarissa Willers1Sias Hamman2Christiaan Malherbe3Jan Steenekamp4Centre of Excellence for Pharmaceutical Sciences, North-West University, Private Bag X6001, Potchefstroom 2520, South AfricaCentre of Excellence for Pharmaceutical Sciences, North-West University, Private Bag X6001, Potchefstroom 2520, South AfricaCentre of Excellence for Pharmaceutical Sciences, North-West University, Private Bag X6001, Potchefstroom 2520, South AfricaPlant Bioactives Group, Post-Harvest and Agro-Processing Technologies, Agricultural Research Council (ARC), Infruitec-Nietvoorbij, Stellenbosch 7599, South AfricaCentre of Excellence for Pharmaceutical Sciences, North-West University, Private Bag X6001, Potchefstroom 2520, South AfricaThe traditional use of <i>Aloe</i> spp. for the purpose of wound healing has a long history and is widespread internationally. Recently, a hybrid aloe plant (<i>Aloe muth-muth</i>) has been cultivated by cross pollination between <i>Aloe vera</i> and <i>Aloe ferox</i>. The <i>Aloe muth-muth</i> plant has not yet been investigated for medicinal properties and provides an opportunity for potential biological activity, including wound healing. The aim of this study was to investigate the in vitro wound healing effects of both <i>Aloe muth-muth</i> gel and whole leaf material with the use of the immortalized human keratinocyte (HaCaT) cell line. Cell viability was conducted using methyl thiazolyl tetrazolium (MTT) assays. In vitro wound healing was tested on HaCaT cells using an established scratch assay method. The effect of <i>Aloe muth-muth</i> gel material on HaCaT cell migration was also investigated. <i>Aloe muth-muth</i> gel material exhibited statistically significantly (<i>p</i> < 0.05) higher percentage wound closure compared to the control at all three concentrations investigated. These findings confirm that this newly cultivated species, <i>Aloe muth-muth</i>, also possesses wound healing activity corresponding to that reported for the two species it is derived from, namely, <i>Aloe vera</i> and <i>Aloe ferox</i>. Therefore, <i>Aloe muth-muth</i> has the potential to be used in future wound therapeutics.https://www.mdpi.com/2079-7737/9/11/350<i>Aloe muth-muth</i>cell migrationHaCaT cellsin vitro cytotoxicityscratch assaywound healing |
| spellingShingle | Morné Fouché Clarissa Willers Sias Hamman Christiaan Malherbe Jan Steenekamp Wound Healing Effects of <i>Aloe muth-muth</i>: In Vitro Investigations Using Immortalized Human Keratinocytes (HaCaT) Biology <i>Aloe muth-muth</i> cell migration HaCaT cells in vitro cytotoxicity scratch assay wound healing |
| title | Wound Healing Effects of <i>Aloe muth-muth</i>: In Vitro Investigations Using Immortalized Human Keratinocytes (HaCaT) |
| title_full | Wound Healing Effects of <i>Aloe muth-muth</i>: In Vitro Investigations Using Immortalized Human Keratinocytes (HaCaT) |
| title_fullStr | Wound Healing Effects of <i>Aloe muth-muth</i>: In Vitro Investigations Using Immortalized Human Keratinocytes (HaCaT) |
| title_full_unstemmed | Wound Healing Effects of <i>Aloe muth-muth</i>: In Vitro Investigations Using Immortalized Human Keratinocytes (HaCaT) |
| title_short | Wound Healing Effects of <i>Aloe muth-muth</i>: In Vitro Investigations Using Immortalized Human Keratinocytes (HaCaT) |
| title_sort | wound healing effects of i aloe muth muth i in vitro investigations using immortalized human keratinocytes hacat |
| topic | <i>Aloe muth-muth</i> cell migration HaCaT cells in vitro cytotoxicity scratch assay wound healing |
| url | https://www.mdpi.com/2079-7737/9/11/350 |
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